Behavior of Cell on Micro Ridge Pattern After Continuous Stimulation of Tangential Force
The behavior of each cell on the micro ridge pattern after the continuous stimulation by a tangential force has been studied <em>in vitro</em>. The stripe-pattern (0.7 μm height, 3 μm width, and 3 μm interval) was made on the surface of the scaffold plate to control the orientation of ea...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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International Institute of Informatics and Cybernetics
2021-06-01
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| Series: | Journal of Systemics, Cybernetics and Informatics |
| Subjects: | |
| Online Access: | http://www.iiisci.org/Journal/PDV/sci/pdfs/ZA088US21.pdf
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| _version_ | 1817999970474655744 |
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| author | Shigehiro Hashimoto Yu Morita Hiroki Yonezawa Yuji Endo |
| author_facet | Shigehiro Hashimoto Yu Morita Hiroki Yonezawa Yuji Endo |
| author_sort | Shigehiro Hashimoto |
| collection | DOAJ |
| description | The behavior of each cell on the micro ridge pattern after the continuous stimulation by a tangential force has been studied <em>in vitro</em>. The stripe-pattern (0.7 μm height, 3 μm width, and 3 μm interval) was made on the surface of the scaffold plate to control the orientation of each cell during the force field stimulation. Variation was made on the angle between the longitudinal direction of the ridge and the direction of the tangential force: 0 degrees, 45 degrees, and 90 degrees. Myoblast (C2C12: mouse myoblast cell line) was used in the experiment. To apply the tangential force field to the cells, the scaffold plate was set in the tube in a conventional centrifugal machine placed in an incubator. After centrifugation for 5 hours, the behavior of each cell at the time-lapse microscope images was traced for 10 hours. The experimental results show that cells on the ridge-lines oriented perpendicular to the tangential force field have the higher activity (migration, and deformation) after stimulation. |
| first_indexed | 2024-04-14T03:16:17Z |
| format | Article |
| id | doaj.art-c6b917beec724830a3892811743b482a |
| institution | Directory Open Access Journal |
| issn | 1690-4524 |
| language | English |
| last_indexed | 2024-04-14T03:16:17Z |
| publishDate | 2021-06-01 |
| publisher | International Institute of Informatics and Cybernetics |
| record_format | Article |
| series | Journal of Systemics, Cybernetics and Informatics |
| spelling | doaj.art-c6b917beec724830a3892811743b482a2022-12-22T02:15:28ZengInternational Institute of Informatics and CyberneticsJournal of Systemics, Cybernetics and Informatics1690-45242021-06-0119317Behavior of Cell on Micro Ridge Pattern After Continuous Stimulation of Tangential ForceShigehiro HashimotoYu MoritaHiroki YonezawaYuji EndoThe behavior of each cell on the micro ridge pattern after the continuous stimulation by a tangential force has been studied <em>in vitro</em>. The stripe-pattern (0.7 μm height, 3 μm width, and 3 μm interval) was made on the surface of the scaffold plate to control the orientation of each cell during the force field stimulation. Variation was made on the angle between the longitudinal direction of the ridge and the direction of the tangential force: 0 degrees, 45 degrees, and 90 degrees. Myoblast (C2C12: mouse myoblast cell line) was used in the experiment. To apply the tangential force field to the cells, the scaffold plate was set in the tube in a conventional centrifugal machine placed in an incubator. After centrifugation for 5 hours, the behavior of each cell at the time-lapse microscope images was traced for 10 hours. The experimental results show that cells on the ridge-lines oriented perpendicular to the tangential force field have the higher activity (migration, and deformation) after stimulation.http://www.iiisci.org/Journal/PDV/sci/pdfs/ZA088US21.pdf biomedical engineeringcell culturecell culturedivision and c2c12tangential force fieldtangential force fielddivision and c2c12 |
| spellingShingle | Shigehiro Hashimoto Yu Morita Hiroki Yonezawa Yuji Endo Behavior of Cell on Micro Ridge Pattern After Continuous Stimulation of Tangential Force Journal of Systemics, Cybernetics and Informatics biomedical engineering cell culture cell culture division and c2c12 tangential force field tangential force field division and c2c12 |
| title | Behavior of Cell on Micro Ridge Pattern After Continuous Stimulation of Tangential Force |
| title_full | Behavior of Cell on Micro Ridge Pattern After Continuous Stimulation of Tangential Force |
| title_fullStr | Behavior of Cell on Micro Ridge Pattern After Continuous Stimulation of Tangential Force |
| title_full_unstemmed | Behavior of Cell on Micro Ridge Pattern After Continuous Stimulation of Tangential Force |
| title_short | Behavior of Cell on Micro Ridge Pattern After Continuous Stimulation of Tangential Force |
| title_sort | behavior of cell on micro ridge pattern after continuous stimulation of tangential force |
| topic | biomedical engineering cell culture cell culture division and c2c12 tangential force field tangential force field division and c2c12 |
| url | http://www.iiisci.org/Journal/PDV/sci/pdfs/ZA088US21.pdf
|
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