Evaluation of staining techniques for the observation of growth bands in tropical elasmobranch vertebrae
The aim of this study was to assess the suitability of different vertebrae staining techniques for the visualization and counting of growth bands in tropical species of batoids (Narcine leoparda, Urotrygon aspidura, Hypanus longus, Potamotrygon magdalenae) and sharks (Alopias pelagicus, Carcharhinus...
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| Format: | Article |
| Language: | English |
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Consejo Superior de Investigaciones Científicas
2020-12-01
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| Series: | Scientia Marina |
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| Online Access: | http://scientiamarina.revistas.csic.es/index.php/scientiamarina/article/view/1876 |
| _version_ | 1819297226735747072 |
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| author | José G. Pérez-Rojas Katherine Torres-Palacios Amalia Uribe Andrés F. Navia Paola A. Mejía-Falla |
| author_facet | José G. Pérez-Rojas Katherine Torres-Palacios Amalia Uribe Andrés F. Navia Paola A. Mejía-Falla |
| author_sort | José G. Pérez-Rojas |
| collection | DOAJ |
| description | The aim of this study was to assess the suitability of different vertebrae staining techniques for the visualization and counting of growth bands in tropical species of batoids (Narcine leoparda, Urotrygon aspidura, Hypanus longus, Potamotrygon magdalenae) and sharks (Alopias pelagicus, Carcharhinus falciformis, Sphyrna lewini, Sphyrna corona and Mustelus lunulatus). Different cutting thicknesses and staining protocols were tested, analysing the precision and bias of each combination to identify the most accurate technique for estimating age. Vertebral sections of 0.4 mm were more suitable for batoids, except for Narcine leoparda; for this species and for all the shark species assessed, sections of 0.5 mm are recommended. Different combinations of stain and exposure time were required to achieve the best visualizations of vertebral growth band pair for the shark and ray species. Intraspecific variation occurred among vertebrae size of batoids. Our results confirm the importance of defining a suitable species-specific protocol for sectioning and staining hard structures before carrying out an age and growth study to improve the reliability of the age estimates. |
| first_indexed | 2024-12-24T05:10:39Z |
| format | Article |
| id | doaj.art-c6ba72b28c6048ffbda8afc0a706f6ea |
| institution | Directory Open Access Journal |
| issn | 0214-8358 1886-8134 |
| language | English |
| last_indexed | 2024-12-24T05:10:39Z |
| publishDate | 2020-12-01 |
| publisher | Consejo Superior de Investigaciones Científicas |
| record_format | Article |
| series | Scientia Marina |
| spelling | doaj.art-c6ba72b28c6048ffbda8afc0a706f6ea2022-12-21T17:13:42ZengConsejo Superior de Investigaciones CientíficasScientia Marina0214-83581886-81342020-12-0184434335410.3989/scimar.05045.03A1834Evaluation of staining techniques for the observation of growth bands in tropical elasmobranch vertebraeJosé G. Pérez-Rojas0https://orcid.org/0000-0002-5515-968XKatherine Torres-Palacios1https://orcid.org/0000-0002-4595-3679Amalia Uribe2https://orcid.org/0000-0002-2378-5512Andrés F. Navia3https://orcid.org/0000-0002-6758-7729Paola A. Mejía-Falla4https://orcid.org/0000-0003-2220-6969Fundación colombiana para la investigación y conservación de tiburones y rayas, SQUALUSFundación colombiana para la investigación y conservación de tiburones y rayas, SQUALUS - Centro Interdisciplinario de Ciencias Marinas, Instituto Politécnico NacionalGrupo Biología CES, Programa Biología, Universidad CESFundación colombiana para la investigación y conservación de tiburones y rayas, SQUALUS - Grupo de Investigación en Ecología, Universidad del ValleFundación colombiana para la investigación y conservación de tiburones y rayas, SQUALUS - Grupo de Investigación en Ecología, Universidad del ValleThe aim of this study was to assess the suitability of different vertebrae staining techniques for the visualization and counting of growth bands in tropical species of batoids (Narcine leoparda, Urotrygon aspidura, Hypanus longus, Potamotrygon magdalenae) and sharks (Alopias pelagicus, Carcharhinus falciformis, Sphyrna lewini, Sphyrna corona and Mustelus lunulatus). Different cutting thicknesses and staining protocols were tested, analysing the precision and bias of each combination to identify the most accurate technique for estimating age. Vertebral sections of 0.4 mm were more suitable for batoids, except for Narcine leoparda; for this species and for all the shark species assessed, sections of 0.5 mm are recommended. Different combinations of stain and exposure time were required to achieve the best visualizations of vertebral growth band pair for the shark and ray species. Intraspecific variation occurred among vertebrae size of batoids. Our results confirm the importance of defining a suitable species-specific protocol for sectioning and staining hard structures before carrying out an age and growth study to improve the reliability of the age estimates.http://scientiamarina.revistas.csic.es/index.php/scientiamarina/article/view/1876agegrowthsharksbatoidsfreshwater stingrayprecisionbias |
| spellingShingle | José G. Pérez-Rojas Katherine Torres-Palacios Amalia Uribe Andrés F. Navia Paola A. Mejía-Falla Evaluation of staining techniques for the observation of growth bands in tropical elasmobranch vertebrae Scientia Marina age growth sharks batoids freshwater stingray precision bias |
| title | Evaluation of staining techniques for the observation of growth bands in tropical elasmobranch vertebrae |
| title_full | Evaluation of staining techniques for the observation of growth bands in tropical elasmobranch vertebrae |
| title_fullStr | Evaluation of staining techniques for the observation of growth bands in tropical elasmobranch vertebrae |
| title_full_unstemmed | Evaluation of staining techniques for the observation of growth bands in tropical elasmobranch vertebrae |
| title_short | Evaluation of staining techniques for the observation of growth bands in tropical elasmobranch vertebrae |
| title_sort | evaluation of staining techniques for the observation of growth bands in tropical elasmobranch vertebrae |
| topic | age growth sharks batoids freshwater stingray precision bias |
| url | http://scientiamarina.revistas.csic.es/index.php/scientiamarina/article/view/1876 |
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