Application of Rhizopus microsporus and Aspergillus oryzae to enhance the defense capacity of eggplant seedlings against Meloidogyne incognita
Several phytopathogens attack eggplant, causing crop damage. One of the most destructive plant diseases, Root-Knot Nematode (RKN), causes significant damage to eggplant seedlings. Finding safe and effective biological alternatives to prevent eggplant root nematode disease, which significantly limit...
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| Format: | Article |
| Language: | English |
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AcademicPres
2023-09-01
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| Series: | Notulae Botanicae Horti Agrobotanici Cluj-Napoca |
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| Online Access: | https://www.notulaebotanicae.ro/index.php/nbha/article/view/13300 |
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| author | Mohamed S. ATTIA Mohamed H. SHARAF Amr H. HASHEM Amira Y. MAHFOUZ Ghadir E. DAIGHAM Abdulaziz A. AL-ASKAR Hamada ABDELGAWAD Mahmoud S. OMAR Ali E. THABET Mahmoud M. ABDALMOHSEN Yousef R. ELADLY Amer M. ABDELAZIZ |
| author_facet | Mohamed S. ATTIA Mohamed H. SHARAF Amr H. HASHEM Amira Y. MAHFOUZ Ghadir E. DAIGHAM Abdulaziz A. AL-ASKAR Hamada ABDELGAWAD Mahmoud S. OMAR Ali E. THABET Mahmoud M. ABDALMOHSEN Yousef R. ELADLY Amer M. ABDELAZIZ |
| author_sort | Mohamed S. ATTIA |
| collection | DOAJ |
| description |
Several phytopathogens attack eggplant, causing crop damage. One of the most destructive plant diseases, Root-Knot Nematode (RKN), causes significant damage to eggplant seedlings. Finding safe and effective biological alternatives to prevent eggplant root nematode disease, which significantly limits plant productivity, is the innovative aspect of this study. Six isolates of plant growth-promoting fungus (PGPF) were tested in the current work for improving biochemical defense and physio-biochemical performance in eggplant seedlings under the Meloidogyne incognita challenge. PGPF isolates were tested in vitro for some biochemical traits such as Siderophores and HCN production. Besides, the antagonistic efficacy of PGPF filtrates against M. incognita was tested in vitro. The best isolates capable of producing HCN were F5 and F3 respectively. Also, F5 followed by F3 exhibited the maximum mortality proportions of 74.20% and 60.35% mortality in nematode juveniles after 96 hours respectively. Moreover, F5 has the highest level of antioxidant activity, with IC50 145 µg/mL followed by F3 with IC50 350 µg/mL. thus, we identified F5 and F3 completely as Rhizopus microsporus (OQ291571.1 and Aspergillus oryzae OQ291572.1. Implementing R. microsporus and A. oryzae collectively in vivo study was the most successful therapy, limiting nematode recordings as 95.23%, 86.98%, 80.35%, 80%, and 68.78% reduction in females, galls, developmental stage, egg masses, second juveniles, respectively, in diseased seedlings. It could be suggested that the use of ethyl acetate extracts (EAE) of A. oryzae and R. microsporus might be commercially applied as a stimulator of eggplant and or anti-nematodes against M. incognita.
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| first_indexed | 2024-03-11T21:04:56Z |
| format | Article |
| id | doaj.art-c6bec28dce7b47009597090e70264f72 |
| institution | Directory Open Access Journal |
| issn | 0255-965X 1842-4309 |
| language | English |
| last_indexed | 2024-03-11T21:04:56Z |
| publishDate | 2023-09-01 |
| publisher | AcademicPres |
| record_format | Article |
| series | Notulae Botanicae Horti Agrobotanici Cluj-Napoca |
| spelling | doaj.art-c6bec28dce7b47009597090e70264f722023-09-29T14:36:28ZengAcademicPresNotulae Botanicae Horti Agrobotanici Cluj-Napoca0255-965X1842-43092023-09-0151310.15835/nbha51313300Application of Rhizopus microsporus and Aspergillus oryzae to enhance the defense capacity of eggplant seedlings against Meloidogyne incognitaMohamed S. ATTIA0Mohamed H. SHARAF1Amr H. HASHEM2Amira Y. MAHFOUZ3Ghadir E. DAIGHAM4Abdulaziz A. AL-ASKAR5Hamada ABDELGAWAD6Mahmoud S. OMAR7Ali E. THABET8Mahmoud M. ABDALMOHSEN9Yousef R. ELADLY10Amer M. ABDELAZIZ11Al-Azhar University, Faculty of Science, Botany and Microbiology Department, CairoAl-Azhar University, Faculty of Science, Botany and Microbiology Department, CairoAl-Azhar University, Faculty of Science, Botany and Microbiology Department, CairoAl-Azhar University, Faculty of Science, Botany and Microbiology Department (Girls Branch) CairoAl-Azhar University, Faculty of Science, Botany and Microbiology Department (Girls Branch) CairoKing Saud University, Faculty of Science, Department of Botany and Microbiology, RiyadhIntegrated Molecular Plant Physiology Research, Department of Biology, University of Antwerp, 2020 AntwerpAl-Azhar University, Faculty of Science, Botany and Microbiology Department, CairoAl-Azhar University, Faculty of Science, Botany and Microbiology Department, CairoAl-Azhar University, Faculty of Science, Botany and Microbiology Department, CairoAl-Azhar University, Faculty of Science, Botany and Microbiology Department, CairoAl-Azhar University, Faculty of Science, Botany and Microbiology Department, Cairo Several phytopathogens attack eggplant, causing crop damage. One of the most destructive plant diseases, Root-Knot Nematode (RKN), causes significant damage to eggplant seedlings. Finding safe and effective biological alternatives to prevent eggplant root nematode disease, which significantly limits plant productivity, is the innovative aspect of this study. Six isolates of plant growth-promoting fungus (PGPF) were tested in the current work for improving biochemical defense and physio-biochemical performance in eggplant seedlings under the Meloidogyne incognita challenge. PGPF isolates were tested in vitro for some biochemical traits such as Siderophores and HCN production. Besides, the antagonistic efficacy of PGPF filtrates against M. incognita was tested in vitro. The best isolates capable of producing HCN were F5 and F3 respectively. Also, F5 followed by F3 exhibited the maximum mortality proportions of 74.20% and 60.35% mortality in nematode juveniles after 96 hours respectively. Moreover, F5 has the highest level of antioxidant activity, with IC50 145 µg/mL followed by F3 with IC50 350 µg/mL. thus, we identified F5 and F3 completely as Rhizopus microsporus (OQ291571.1 and Aspergillus oryzae OQ291572.1. Implementing R. microsporus and A. oryzae collectively in vivo study was the most successful therapy, limiting nematode recordings as 95.23%, 86.98%, 80.35%, 80%, and 68.78% reduction in females, galls, developmental stage, egg masses, second juveniles, respectively, in diseased seedlings. It could be suggested that the use of ethyl acetate extracts (EAE) of A. oryzae and R. microsporus might be commercially applied as a stimulator of eggplant and or anti-nematodes against M. incognita. https://www.notulaebotanicae.ro/index.php/nbha/article/view/13300Aspergillus oryzaebiochemical defenseeggplantfunginematicidalRhizopus microspores |
| spellingShingle | Mohamed S. ATTIA Mohamed H. SHARAF Amr H. HASHEM Amira Y. MAHFOUZ Ghadir E. DAIGHAM Abdulaziz A. AL-ASKAR Hamada ABDELGAWAD Mahmoud S. OMAR Ali E. THABET Mahmoud M. ABDALMOHSEN Yousef R. ELADLY Amer M. ABDELAZIZ Application of Rhizopus microsporus and Aspergillus oryzae to enhance the defense capacity of eggplant seedlings against Meloidogyne incognita Notulae Botanicae Horti Agrobotanici Cluj-Napoca Aspergillus oryzae biochemical defense eggplant fungi nematicidal Rhizopus microspores |
| title | Application of Rhizopus microsporus and Aspergillus oryzae to enhance the defense capacity of eggplant seedlings against Meloidogyne incognita |
| title_full | Application of Rhizopus microsporus and Aspergillus oryzae to enhance the defense capacity of eggplant seedlings against Meloidogyne incognita |
| title_fullStr | Application of Rhizopus microsporus and Aspergillus oryzae to enhance the defense capacity of eggplant seedlings against Meloidogyne incognita |
| title_full_unstemmed | Application of Rhizopus microsporus and Aspergillus oryzae to enhance the defense capacity of eggplant seedlings against Meloidogyne incognita |
| title_short | Application of Rhizopus microsporus and Aspergillus oryzae to enhance the defense capacity of eggplant seedlings against Meloidogyne incognita |
| title_sort | application of rhizopus microsporus and aspergillus oryzae to enhance the defense capacity of eggplant seedlings against meloidogyne incognita |
| topic | Aspergillus oryzae biochemical defense eggplant fungi nematicidal Rhizopus microspores |
| url | https://www.notulaebotanicae.ro/index.php/nbha/article/view/13300 |
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