Identification of Pathogenic Candida Species: PCR-Fragment Size Polymorphism (PCR-FSP) Method
"nBackground: The clinical importance of yeast infections has increased in recent decades. There are 10-15 pathogenic Candida species. The current morphological and physiological methods for identification of Candida species are generally not easy to interpret and may be expensive or time-c...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | fas |
| Published: |
Tehran University of Medical Sciences
2008-12-01
|
| Series: | Tehran University Medical Journal |
| Subjects: | |
| Online Access: | http://journals.tums.ac.ir/PdfMed.aspx?pdf_med=/upload_files/pdf/12328.pdf&manuscript_id=12328 |
| _version_ | 1819094817820377088 |
|---|---|
| author | Mirhendi SH Adin H Shidfar MR Kordbacheh P Hashemi SJ Moazeni M Hosseinpur L Rezaie Matehkolaie A |
| author_facet | Mirhendi SH Adin H Shidfar MR Kordbacheh P Hashemi SJ Moazeni M Hosseinpur L Rezaie Matehkolaie A |
| author_sort | Mirhendi SH |
| collection | DOAJ |
| description | "nBackground: The clinical importance of yeast infections has increased in recent decades. There are 10-15 pathogenic Candida species. The current morphological and physiological methods for identification of Candida species are generally not easy to interpret and may be expensive or time-consuming. In the present study, we introduce and use a new approach for the identification and differentiation of medically important yeast species of Candida. In this method, size polymorphism of the internal transcribed spacer regions, ITS1 and ITS2, of the ribosomal DNA in various Candida species is used as the basis of species recognition. "nMethods: The genomic DNA of 31 standard strains and 60 clinical isolates was extracted and PCR-amplified using two primer pairs (ITS1-ITS2 and ITS3-ITS4) separately. Both PCR products were mixed and analyzed after standard agarose gel electrophoresis. The species of the tested yeasts were identified by the electrophoretic patterns of the mixed PCR products of each sample, comparing the data obtained from the sequence analyses of ITS1 and ITS2 molecules. "nResults: By this method, with the exception of C. albicans and C. dubliniensis, we were able to clearly differentiate nearly all common pathogenic Candida species, including C. albicans, C. glabrata, C. gulliermondii, C. parapsilosis, C. tropicalis,      C. krusei, C. kefyr, C. lusinaniae and C. rugosa. All standard and clinical strains were identified correctly, without expensive methods such as sequencing and capillary electrophoresis. "nConclusion: It seems that the PCR-FSP method introduced in this study is the easiest molecular approach for the identification of a wide range of pathogenic Candida species and is applicable for diagnostic and epidemiological purposes in reference laboratories. |
| first_indexed | 2024-12-21T23:33:26Z |
| format | Article |
| id | doaj.art-c6bf0d3bf4e645d4ad206f4ffc86d3f9 |
| institution | Directory Open Access Journal |
| issn | 1683-1764 1735-7322 |
| language | fas |
| last_indexed | 2024-12-21T23:33:26Z |
| publishDate | 2008-12-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Tehran University Medical Journal |
| spelling | doaj.art-c6bf0d3bf4e645d4ad206f4ffc86d3f92022-12-21T18:46:26ZfasTehran University of Medical SciencesTehran University Medical Journal1683-17641735-73222008-12-01669639645Identification of Pathogenic Candida Species: PCR-Fragment Size Polymorphism (PCR-FSP) MethodMirhendi SHAdin HShidfar MRKordbacheh PHashemi SJMoazeni MHosseinpur LRezaie Matehkolaie A"nBackground: The clinical importance of yeast infections has increased in recent decades. There are 10-15 pathogenic Candida species. The current morphological and physiological methods for identification of Candida species are generally not easy to interpret and may be expensive or time-consuming. In the present study, we introduce and use a new approach for the identification and differentiation of medically important yeast species of Candida. In this method, size polymorphism of the internal transcribed spacer regions, ITS1 and ITS2, of the ribosomal DNA in various Candida species is used as the basis of species recognition. "nMethods: The genomic DNA of 31 standard strains and 60 clinical isolates was extracted and PCR-amplified using two primer pairs (ITS1-ITS2 and ITS3-ITS4) separately. Both PCR products were mixed and analyzed after standard agarose gel electrophoresis. The species of the tested yeasts were identified by the electrophoretic patterns of the mixed PCR products of each sample, comparing the data obtained from the sequence analyses of ITS1 and ITS2 molecules. "nResults: By this method, with the exception of C. albicans and C. dubliniensis, we were able to clearly differentiate nearly all common pathogenic Candida species, including C. albicans, C. glabrata, C. gulliermondii, C. parapsilosis, C. tropicalis,      C. krusei, C. kefyr, C. lusinaniae and C. rugosa. All standard and clinical strains were identified correctly, without expensive methods such as sequencing and capillary electrophoresis. "nConclusion: It seems that the PCR-FSP method introduced in this study is the easiest molecular approach for the identification of a wide range of pathogenic Candida species and is applicable for diagnostic and epidemiological purposes in reference laboratories.http://journals.tums.ac.ir/PdfMed.aspx?pdf_med=/upload_files/pdf/12328.pdf&manuscript_id=12328PCR-FSPcandidaidentificationITS |
| spellingShingle | Mirhendi SH Adin H Shidfar MR Kordbacheh P Hashemi SJ Moazeni M Hosseinpur L Rezaie Matehkolaie A Identification of Pathogenic Candida Species: PCR-Fragment Size Polymorphism (PCR-FSP) Method Tehran University Medical Journal PCR-FSP candida identification ITS |
| title | Identification of Pathogenic Candida Species: PCR-Fragment Size Polymorphism (PCR-FSP) Method |
| title_full | Identification of Pathogenic Candida Species: PCR-Fragment Size Polymorphism (PCR-FSP) Method |
| title_fullStr | Identification of Pathogenic Candida Species: PCR-Fragment Size Polymorphism (PCR-FSP) Method |
| title_full_unstemmed | Identification of Pathogenic Candida Species: PCR-Fragment Size Polymorphism (PCR-FSP) Method |
| title_short | Identification of Pathogenic Candida Species: PCR-Fragment Size Polymorphism (PCR-FSP) Method |
| title_sort | identification of pathogenic candida species pcr fragment size polymorphism pcr fsp method |
| topic | PCR-FSP candida identification ITS |
| url | http://journals.tums.ac.ir/PdfMed.aspx?pdf_med=/upload_files/pdf/12328.pdf&manuscript_id=12328 |
| work_keys_str_mv | AT mirhendish identificationofpathogeniccandidaspeciespcrfragmentsizepolymorphismpcrfspmethod AT adinh identificationofpathogeniccandidaspeciespcrfragmentsizepolymorphismpcrfspmethod AT shidfarmr identificationofpathogeniccandidaspeciespcrfragmentsizepolymorphismpcrfspmethod AT kordbachehp identificationofpathogeniccandidaspeciespcrfragmentsizepolymorphismpcrfspmethod AT hashemisj identificationofpathogeniccandidaspeciespcrfragmentsizepolymorphismpcrfspmethod AT moazenim identificationofpathogeniccandidaspeciespcrfragmentsizepolymorphismpcrfspmethod AT hosseinpurl identificationofpathogeniccandidaspeciespcrfragmentsizepolymorphismpcrfspmethod AT rezaiematehkolaiea identificationofpathogeniccandidaspeciespcrfragmentsizepolymorphismpcrfspmethod |