Puerarin Reduces Oxidative Damage and Photoaging Caused by UVA Radiation in Human Fibroblasts by Regulating Nrf2 and MAPK Signaling Pathways
Fibroblasts account for more than 95% of dermal cells maintaining dermal structure and function. However, UVA penetrates the dermis and causes oxidative stress that damages the dermis and accelerates skin aging. Puerarin, the main active ingredient of <i>Puerariae lobata</i>, has been de...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-11-01
|
| Series: | Nutrients |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2072-6643/14/22/4724 |
| _version_ | 1797464314257342464 |
|---|---|
| author | Qiuting Mo Shuping Li Shiquan You Dongdong Wang Jiachan Zhang Meng Li Changtao Wang |
| author_facet | Qiuting Mo Shuping Li Shiquan You Dongdong Wang Jiachan Zhang Meng Li Changtao Wang |
| author_sort | Qiuting Mo |
| collection | DOAJ |
| description | Fibroblasts account for more than 95% of dermal cells maintaining dermal structure and function. However, UVA penetrates the dermis and causes oxidative stress that damages the dermis and accelerates skin aging. Puerarin, the main active ingredient of <i>Puerariae lobata</i>, has been demonstrated to withstand oxidative stress caused by a variety of factors. However, there are limited findings on whether puerarin protects fibroblasts from UVA-induced oxidative stress damage. The effects of puerarin on human skin fibroblasts (HSF) under UVA-induced oxidative stress were investigated in this study. It is found that puerarin upregulates antioxidant enzymes’ mRNA expression level and their content through modulating the KEAP1-Nrf2/ARE signaling pathway, thus improving cell antioxidant capacity and successfully eliminating UVA-induced reactive oxygen species (ROS) and lipid oxidation product malondialdehyde (MDA). Additionally, puerarin blocks the overexpression of human extracellular signal-regulated kinase (ERK), human c-Jun amino-terminal kinase (JNK), and P38, which downregulates matrix metalloproteinase 1 (MMP-1) expression and increases type I collagen (COL-1) expression. Moreover, preliminary research on mouse skin suggests that puerarin can hydrate, moisturize, and increase the antioxidant capacity of skin tissue. These findings suggest that puerarin can protect the skin against photoaging. |
| first_indexed | 2024-03-09T18:06:21Z |
| format | Article |
| id | doaj.art-c6c0dcd8b430455d98a833ba6bc0e2bd |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-09T18:06:21Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-c6c0dcd8b430455d98a833ba6bc0e2bd2023-11-24T09:30:06ZengMDPI AGNutrients2072-66432022-11-011422472410.3390/nu14224724Puerarin Reduces Oxidative Damage and Photoaging Caused by UVA Radiation in Human Fibroblasts by Regulating Nrf2 and MAPK Signaling PathwaysQiuting Mo0Shuping Li1Shiquan You2Dongdong Wang3Jiachan Zhang4Meng Li5Changtao Wang6Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University, Fucheng Road, Beijing 100048, ChinaBeijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University, Fucheng Road, Beijing 100048, ChinaBeijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University, Fucheng Road, Beijing 100048, ChinaBeijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University, Fucheng Road, Beijing 100048, ChinaBeijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University, Fucheng Road, Beijing 100048, ChinaBeijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University, Fucheng Road, Beijing 100048, ChinaBeijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University, Fucheng Road, Beijing 100048, ChinaFibroblasts account for more than 95% of dermal cells maintaining dermal structure and function. However, UVA penetrates the dermis and causes oxidative stress that damages the dermis and accelerates skin aging. Puerarin, the main active ingredient of <i>Puerariae lobata</i>, has been demonstrated to withstand oxidative stress caused by a variety of factors. However, there are limited findings on whether puerarin protects fibroblasts from UVA-induced oxidative stress damage. The effects of puerarin on human skin fibroblasts (HSF) under UVA-induced oxidative stress were investigated in this study. It is found that puerarin upregulates antioxidant enzymes’ mRNA expression level and their content through modulating the KEAP1-Nrf2/ARE signaling pathway, thus improving cell antioxidant capacity and successfully eliminating UVA-induced reactive oxygen species (ROS) and lipid oxidation product malondialdehyde (MDA). Additionally, puerarin blocks the overexpression of human extracellular signal-regulated kinase (ERK), human c-Jun amino-terminal kinase (JNK), and P38, which downregulates matrix metalloproteinase 1 (MMP-1) expression and increases type I collagen (COL-1) expression. Moreover, preliminary research on mouse skin suggests that puerarin can hydrate, moisturize, and increase the antioxidant capacity of skin tissue. These findings suggest that puerarin can protect the skin against photoaging.https://www.mdpi.com/2072-6643/14/22/4724puerarinoxidative stressantioxidantanti-photoaging |
| spellingShingle | Qiuting Mo Shuping Li Shiquan You Dongdong Wang Jiachan Zhang Meng Li Changtao Wang Puerarin Reduces Oxidative Damage and Photoaging Caused by UVA Radiation in Human Fibroblasts by Regulating Nrf2 and MAPK Signaling Pathways Nutrients puerarin oxidative stress antioxidant anti-photoaging |
| title | Puerarin Reduces Oxidative Damage and Photoaging Caused by UVA Radiation in Human Fibroblasts by Regulating Nrf2 and MAPK Signaling Pathways |
| title_full | Puerarin Reduces Oxidative Damage and Photoaging Caused by UVA Radiation in Human Fibroblasts by Regulating Nrf2 and MAPK Signaling Pathways |
| title_fullStr | Puerarin Reduces Oxidative Damage and Photoaging Caused by UVA Radiation in Human Fibroblasts by Regulating Nrf2 and MAPK Signaling Pathways |
| title_full_unstemmed | Puerarin Reduces Oxidative Damage and Photoaging Caused by UVA Radiation in Human Fibroblasts by Regulating Nrf2 and MAPK Signaling Pathways |
| title_short | Puerarin Reduces Oxidative Damage and Photoaging Caused by UVA Radiation in Human Fibroblasts by Regulating Nrf2 and MAPK Signaling Pathways |
| title_sort | puerarin reduces oxidative damage and photoaging caused by uva radiation in human fibroblasts by regulating nrf2 and mapk signaling pathways |
| topic | puerarin oxidative stress antioxidant anti-photoaging |
| url | https://www.mdpi.com/2072-6643/14/22/4724 |
| work_keys_str_mv | AT qiutingmo puerarinreducesoxidativedamageandphotoagingcausedbyuvaradiationinhumanfibroblastsbyregulatingnrf2andmapksignalingpathways AT shupingli puerarinreducesoxidativedamageandphotoagingcausedbyuvaradiationinhumanfibroblastsbyregulatingnrf2andmapksignalingpathways AT shiquanyou puerarinreducesoxidativedamageandphotoagingcausedbyuvaradiationinhumanfibroblastsbyregulatingnrf2andmapksignalingpathways AT dongdongwang puerarinreducesoxidativedamageandphotoagingcausedbyuvaradiationinhumanfibroblastsbyregulatingnrf2andmapksignalingpathways AT jiachanzhang puerarinreducesoxidativedamageandphotoagingcausedbyuvaradiationinhumanfibroblastsbyregulatingnrf2andmapksignalingpathways AT mengli puerarinreducesoxidativedamageandphotoagingcausedbyuvaradiationinhumanfibroblastsbyregulatingnrf2andmapksignalingpathways AT changtaowang puerarinreducesoxidativedamageandphotoagingcausedbyuvaradiationinhumanfibroblastsbyregulatingnrf2andmapksignalingpathways |