Color-Flu Fluorescent Reporter Influenza A Viruses Allow for In Vivo Studies of Innate Immune Function in Zebrafish
Influenza virus infection can cause severe respiratory disease and is estimated to cause millions of illnesses annually. Studies on the contribution of the innate immune response to influenza A virus (IAV) to viral pathogenesis may yield new antiviral strategies. Zebrafish larvae are useful models f...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-01-01
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| Series: | Viruses |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1999-4915/16/1/155 |
| _version_ | 1797342405636128768 |
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| author | Brandy-Lee Soos Alec Ballinger Mykayla Weinstein Haley Foreman Julianna Grampone Samuel Weafer Connor Aylesworth Benjamin L. King |
| author_facet | Brandy-Lee Soos Alec Ballinger Mykayla Weinstein Haley Foreman Julianna Grampone Samuel Weafer Connor Aylesworth Benjamin L. King |
| author_sort | Brandy-Lee Soos |
| collection | DOAJ |
| description | Influenza virus infection can cause severe respiratory disease and is estimated to cause millions of illnesses annually. Studies on the contribution of the innate immune response to influenza A virus (IAV) to viral pathogenesis may yield new antiviral strategies. Zebrafish larvae are useful models for studying the innate immune response to pathogens, including IAV, in vivo. Here, we demonstrate how Color-flu, four fluorescent IAV strains originally developed for mice, can be used to study the host response to infection by simultaneously monitoring infected cells, neutrophils, and macrophages in vivo. Using this model, we show how the angiotensin-converting enzyme inhibitor, ramipril, and mitophagy inhibitor, MDIVI-1, improved survival, decreased viral burden, and improved the respiratory burst response to IAV infection. The Color-flu zebrafish larvae model of IAV infection is complementary to other models where the dynamics of infection and the response of innate immune cells can be visualized in a transparent host in vivo. |
| first_indexed | 2024-03-08T10:32:49Z |
| format | Article |
| id | doaj.art-c6c4f384b88349ae90a2eb559a63e048 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-08T10:32:49Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-c6c4f384b88349ae90a2eb559a63e0482024-01-26T18:48:39ZengMDPI AGViruses1999-49152024-01-0116115510.3390/v16010155Color-Flu Fluorescent Reporter Influenza A Viruses Allow for In Vivo Studies of Innate Immune Function in ZebrafishBrandy-Lee Soos0Alec Ballinger1Mykayla Weinstein2Haley Foreman3Julianna Grampone4Samuel Weafer5Connor Aylesworth6Benjamin L. King7Department of Molecular and Biomedical Sciences, University of Maine, Orono, ME 04469, USADepartment of Molecular and Biomedical Sciences, University of Maine, Orono, ME 04469, USADepartment of Molecular and Biomedical Sciences, University of Maine, Orono, ME 04469, USADepartment of Molecular and Biomedical Sciences, University of Maine, Orono, ME 04469, USADepartment of Molecular and Biomedical Sciences, University of Maine, Orono, ME 04469, USADepartment of Molecular and Biomedical Sciences, University of Maine, Orono, ME 04469, USADepartment of Molecular and Biomedical Sciences, University of Maine, Orono, ME 04469, USADepartment of Molecular and Biomedical Sciences, University of Maine, Orono, ME 04469, USAInfluenza virus infection can cause severe respiratory disease and is estimated to cause millions of illnesses annually. Studies on the contribution of the innate immune response to influenza A virus (IAV) to viral pathogenesis may yield new antiviral strategies. Zebrafish larvae are useful models for studying the innate immune response to pathogens, including IAV, in vivo. Here, we demonstrate how Color-flu, four fluorescent IAV strains originally developed for mice, can be used to study the host response to infection by simultaneously monitoring infected cells, neutrophils, and macrophages in vivo. Using this model, we show how the angiotensin-converting enzyme inhibitor, ramipril, and mitophagy inhibitor, MDIVI-1, improved survival, decreased viral burden, and improved the respiratory burst response to IAV infection. The Color-flu zebrafish larvae model of IAV infection is complementary to other models where the dynamics of infection and the response of innate immune cells can be visualized in a transparent host in vivo.https://www.mdpi.com/1999-4915/16/1/155influenza virusinnate immune responsehost–pathogen interactionszebrafish |
| spellingShingle | Brandy-Lee Soos Alec Ballinger Mykayla Weinstein Haley Foreman Julianna Grampone Samuel Weafer Connor Aylesworth Benjamin L. King Color-Flu Fluorescent Reporter Influenza A Viruses Allow for In Vivo Studies of Innate Immune Function in Zebrafish Viruses influenza virus innate immune response host–pathogen interactions zebrafish |
| title | Color-Flu Fluorescent Reporter Influenza A Viruses Allow for In Vivo Studies of Innate Immune Function in Zebrafish |
| title_full | Color-Flu Fluorescent Reporter Influenza A Viruses Allow for In Vivo Studies of Innate Immune Function in Zebrafish |
| title_fullStr | Color-Flu Fluorescent Reporter Influenza A Viruses Allow for In Vivo Studies of Innate Immune Function in Zebrafish |
| title_full_unstemmed | Color-Flu Fluorescent Reporter Influenza A Viruses Allow for In Vivo Studies of Innate Immune Function in Zebrafish |
| title_short | Color-Flu Fluorescent Reporter Influenza A Viruses Allow for In Vivo Studies of Innate Immune Function in Zebrafish |
| title_sort | color flu fluorescent reporter influenza a viruses allow for in vivo studies of innate immune function in zebrafish |
| topic | influenza virus innate immune response host–pathogen interactions zebrafish |
| url | https://www.mdpi.com/1999-4915/16/1/155 |
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