Modeling exercise using optogenetically contractible Drosophila larvae
Abstract The pathophysiological effects of a number of metabolic and age-related disorders can be prevented to some extent by exercise and increased physical activity. However, the molecular mechanisms that contribute to the beneficial effects of muscle activity remain poorly explored. Availability...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-08-01
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| Series: | BMC Genomics |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12864-022-08845-6 |
| _version_ | 1828351914805297152 |
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| author | Arpan C. Ghosh Yanhui Hu Sudhir Gopal Tattikota Yifang Liu Aram Comjean Norbert Perrimon |
| author_facet | Arpan C. Ghosh Yanhui Hu Sudhir Gopal Tattikota Yifang Liu Aram Comjean Norbert Perrimon |
| author_sort | Arpan C. Ghosh |
| collection | DOAJ |
| description | Abstract The pathophysiological effects of a number of metabolic and age-related disorders can be prevented to some extent by exercise and increased physical activity. However, the molecular mechanisms that contribute to the beneficial effects of muscle activity remain poorly explored. Availability of a fast, inexpensive, and genetically tractable model system for muscle activity and exercise will allow the rapid identification and characterization of molecular mechanisms that mediate the beneficial effects of exercise. Here, we report the development and characterization of an optogenetically-inducible muscle contraction (OMC) model in Drosophila larvae that we used to study acute exercise-like physiological responses. To characterize muscle-specific transcriptional responses to acute exercise, we performed bulk mRNA-sequencing, revealing striking similarities between acute exercise-induced genes in flies and those previously identified in humans. Our larval muscle contraction model opens a path for rapid identification and characterization of exercise-induced factors. |
| first_indexed | 2024-04-14T01:46:08Z |
| format | Article |
| id | doaj.art-c6c63ea4d4004b3a9e944b34036b4c48 |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-04-14T01:46:08Z |
| publishDate | 2022-08-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-c6c63ea4d4004b3a9e944b34036b4c482022-12-22T02:19:31ZengBMCBMC Genomics1471-21642022-08-0123111610.1186/s12864-022-08845-6Modeling exercise using optogenetically contractible Drosophila larvaeArpan C. Ghosh0Yanhui Hu1Sudhir Gopal Tattikota2Yifang Liu3Aram Comjean4Norbert Perrimon5Department of Genetics, Blavatnik Institute, Harvard Medical SchoolDepartment of Genetics, Blavatnik Institute, Harvard Medical SchoolDepartment of Genetics, Blavatnik Institute, Harvard Medical SchoolDepartment of Genetics, Blavatnik Institute, Harvard Medical SchoolDepartment of Genetics, Blavatnik Institute, Harvard Medical SchoolDepartment of Genetics, Blavatnik Institute, Harvard Medical SchoolAbstract The pathophysiological effects of a number of metabolic and age-related disorders can be prevented to some extent by exercise and increased physical activity. However, the molecular mechanisms that contribute to the beneficial effects of muscle activity remain poorly explored. Availability of a fast, inexpensive, and genetically tractable model system for muscle activity and exercise will allow the rapid identification and characterization of molecular mechanisms that mediate the beneficial effects of exercise. Here, we report the development and characterization of an optogenetically-inducible muscle contraction (OMC) model in Drosophila larvae that we used to study acute exercise-like physiological responses. To characterize muscle-specific transcriptional responses to acute exercise, we performed bulk mRNA-sequencing, revealing striking similarities between acute exercise-induced genes in flies and those previously identified in humans. Our larval muscle contraction model opens a path for rapid identification and characterization of exercise-induced factors.https://doi.org/10.1186/s12864-022-08845-6DrosophilaExerciseOptogeneticsMuscleRNA-seqMyokines |
| spellingShingle | Arpan C. Ghosh Yanhui Hu Sudhir Gopal Tattikota Yifang Liu Aram Comjean Norbert Perrimon Modeling exercise using optogenetically contractible Drosophila larvae BMC Genomics Drosophila Exercise Optogenetics Muscle RNA-seq Myokines |
| title | Modeling exercise using optogenetically contractible Drosophila larvae |
| title_full | Modeling exercise using optogenetically contractible Drosophila larvae |
| title_fullStr | Modeling exercise using optogenetically contractible Drosophila larvae |
| title_full_unstemmed | Modeling exercise using optogenetically contractible Drosophila larvae |
| title_short | Modeling exercise using optogenetically contractible Drosophila larvae |
| title_sort | modeling exercise using optogenetically contractible drosophila larvae |
| topic | Drosophila Exercise Optogenetics Muscle RNA-seq Myokines |
| url | https://doi.org/10.1186/s12864-022-08845-6 |
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