MALDI-TOF MS Analysis of Cellodextrins and Xylo-oligosaccharides Produced by Hindgut Homogenates of Reticulitermes santonensis
Hindgut homogenates of the termite Reticulitermes santonensis were incubated with carboxymethyl cellulose (CMC), crystalline celluloses or xylan substrates. Hydrolysates were analyzed with matrix-assisted laser desorption/ionization coupled to time-of-flight mass spectrometry (MALDI-TOF MS). The met...
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| Format: | Article |
| Language: | English |
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MDPI AG
2014-04-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/19/4/4578 |
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| author | Catherine Brasseur Julien Bauwens Cédric Tarayre Christel Mattéotti Philippe Thonart Jacqueline Destain Frédéric Francis Eric Haubruge Daniel Portetelle Micheline Vandenbol Jean-François Focant Edwin De Pauw |
| author_facet | Catherine Brasseur Julien Bauwens Cédric Tarayre Christel Mattéotti Philippe Thonart Jacqueline Destain Frédéric Francis Eric Haubruge Daniel Portetelle Micheline Vandenbol Jean-François Focant Edwin De Pauw |
| author_sort | Catherine Brasseur |
| collection | DOAJ |
| description | Hindgut homogenates of the termite Reticulitermes santonensis were incubated with carboxymethyl cellulose (CMC), crystalline celluloses or xylan substrates. Hydrolysates were analyzed with matrix-assisted laser desorption/ionization coupled to time-of-flight mass spectrometry (MALDI-TOF MS). The method was first set up using acid hydrolysis analysis to characterize non-enzymatic profiles. Commercial enzymes of Trichoderma reesei or T. longibrachiatum were also tested to validate the enzymatic hydrolysis analysis. For CMC hydrolysis, data processing and visual display were optimized to obtain comprehensive profiles and allow rapid comparison and evaluation of enzymatic selectivity, according to the number of substituents of each hydrolysis product. Oligosaccharides with degrees of polymerization (DPs) ranging from three to 12 were measured from CMC and the enzymatic selectivity was demonstrated. Neutral and acidic xylo-oligosaccharides with DPs ranging from three to 11 were measured from xylan substrate. These results are of interest for lignocellulose biomass valorization and demonstrated the potential of termites and their symbiotic microbiota as a source of interesting enzymes for oligosaccharides production. |
| first_indexed | 2024-12-21T18:06:06Z |
| format | Article |
| id | doaj.art-c6ca6777f2c14c418dbe36cc7431d117 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-21T18:06:06Z |
| publishDate | 2014-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-c6ca6777f2c14c418dbe36cc7431d1172022-12-21T18:54:56ZengMDPI AGMolecules1420-30492014-04-011944578459410.3390/molecules19044578molecules19044578MALDI-TOF MS Analysis of Cellodextrins and Xylo-oligosaccharides Produced by Hindgut Homogenates of Reticulitermes santonensisCatherine Brasseur0Julien Bauwens1Cédric Tarayre2Christel Mattéotti3Philippe Thonart4Jacqueline Destain5Frédéric Francis6Eric Haubruge7Daniel Portetelle8Micheline Vandenbol9Jean-François Focant10Edwin De Pauw11Mass Spectrometry Laboratory, Department of Chemistry, University of Liège, Allée du Six Aout, B6c, Sart-Tilman, B-4000 Liège, BelgiumDepartment of Functional and Evolutionary Entomology, Gembloux Agro-Bio Tech, University of Liège, 2 Passage des Déportés, B-5030 Gembloux, BelgiumDepartment of Bio-Industries, Gembloux Agro-Bio Tech, University of Liège, 2 Passage des Déportés, B-5030 Gembloux, BelgiumDepartment of Microbiology and Genomics, Gembloux Agro-Bio Tech, University of Liège, 6 Avenue Maréchal Juin, B-5030 Gembloux, BelgiumDepartment of Bio-Industries, Gembloux Agro-Bio Tech, University of Liège, 2 Passage des Déportés, B-5030 Gembloux, BelgiumDepartment of Bio-Industries, Gembloux Agro-Bio Tech, University of Liège, 2 Passage des Déportés, B-5030 Gembloux, BelgiumDepartment of Functional and Evolutionary Entomology, Gembloux Agro-Bio Tech, University of Liège, 2 Passage des Déportés, B-5030 Gembloux, BelgiumDepartment of Functional and Evolutionary Entomology, Gembloux Agro-Bio Tech, University of Liège, 2 Passage des Déportés, B-5030 Gembloux, BelgiumDepartment of Microbiology and Genomics, Gembloux Agro-Bio Tech, University of Liège, 6 Avenue Maréchal Juin, B-5030 Gembloux, BelgiumDepartment of Microbiology and Genomics, Gembloux Agro-Bio Tech, University of Liège, 6 Avenue Maréchal Juin, B-5030 Gembloux, BelgiumOrganic and Biological Analytical Chemistry, Department of Chemistry, University of Liège, Allée du Six Aout, B6c, Sart-Tilman, B-4000 Liège, BelgiumMass Spectrometry Laboratory, Department of Chemistry, University of Liège, Allée du Six Aout, B6c, Sart-Tilman, B-4000 Liège, BelgiumHindgut homogenates of the termite Reticulitermes santonensis were incubated with carboxymethyl cellulose (CMC), crystalline celluloses or xylan substrates. Hydrolysates were analyzed with matrix-assisted laser desorption/ionization coupled to time-of-flight mass spectrometry (MALDI-TOF MS). The method was first set up using acid hydrolysis analysis to characterize non-enzymatic profiles. Commercial enzymes of Trichoderma reesei or T. longibrachiatum were also tested to validate the enzymatic hydrolysis analysis. For CMC hydrolysis, data processing and visual display were optimized to obtain comprehensive profiles and allow rapid comparison and evaluation of enzymatic selectivity, according to the number of substituents of each hydrolysis product. Oligosaccharides with degrees of polymerization (DPs) ranging from three to 12 were measured from CMC and the enzymatic selectivity was demonstrated. Neutral and acidic xylo-oligosaccharides with DPs ranging from three to 11 were measured from xylan substrate. These results are of interest for lignocellulose biomass valorization and demonstrated the potential of termites and their symbiotic microbiota as a source of interesting enzymes for oligosaccharides production.http://www.mdpi.com/1420-3049/19/4/4578xylo-oligosaccharidescellodextrinstermitesMALDI-TOF MSTrichoderma reesei |
| spellingShingle | Catherine Brasseur Julien Bauwens Cédric Tarayre Christel Mattéotti Philippe Thonart Jacqueline Destain Frédéric Francis Eric Haubruge Daniel Portetelle Micheline Vandenbol Jean-François Focant Edwin De Pauw MALDI-TOF MS Analysis of Cellodextrins and Xylo-oligosaccharides Produced by Hindgut Homogenates of Reticulitermes santonensis Molecules xylo-oligosaccharides cellodextrins termites MALDI-TOF MS Trichoderma reesei |
| title | MALDI-TOF MS Analysis of Cellodextrins and Xylo-oligosaccharides Produced by Hindgut Homogenates of Reticulitermes santonensis |
| title_full | MALDI-TOF MS Analysis of Cellodextrins and Xylo-oligosaccharides Produced by Hindgut Homogenates of Reticulitermes santonensis |
| title_fullStr | MALDI-TOF MS Analysis of Cellodextrins and Xylo-oligosaccharides Produced by Hindgut Homogenates of Reticulitermes santonensis |
| title_full_unstemmed | MALDI-TOF MS Analysis of Cellodextrins and Xylo-oligosaccharides Produced by Hindgut Homogenates of Reticulitermes santonensis |
| title_short | MALDI-TOF MS Analysis of Cellodextrins and Xylo-oligosaccharides Produced by Hindgut Homogenates of Reticulitermes santonensis |
| title_sort | maldi tof ms analysis of cellodextrins and xylo oligosaccharides produced by hindgut homogenates of reticulitermes santonensis |
| topic | xylo-oligosaccharides cellodextrins termites MALDI-TOF MS Trichoderma reesei |
| url | http://www.mdpi.com/1420-3049/19/4/4578 |
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