Alterations to the gut microbiome impair bone tissue strength in aged mice
Whole bone strength and resistance to fracture are determined by a combination of bone quantity and bone quality – key factors in determining risk for osteoporosis and age-related fractures. Recent preclinical studies have shown that alterations to the gut microbiome can influence bone quantity as w...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-06-01
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| Series: | Bone Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S235218722100320X |
| _version_ | 1818650922821091328 |
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| author | Macy Castaneda Kelsey M. Smith Jacob C. Nixon Christopher J. Hernandez Sheldon Rowan |
| author_facet | Macy Castaneda Kelsey M. Smith Jacob C. Nixon Christopher J. Hernandez Sheldon Rowan |
| author_sort | Macy Castaneda |
| collection | DOAJ |
| description | Whole bone strength and resistance to fracture are determined by a combination of bone quantity and bone quality – key factors in determining risk for osteoporosis and age-related fractures. Recent preclinical studies have shown that alterations to the gut microbiome can influence bone quantity as well as bone tissue quality. Prior work on the gut microbiome and bone has been limited to young animals, and it is unknown if the gut microbiome can alter bone tissue strength in aged animals. Here we ask if alterations to the constituents of the gut microbiome influence bone strength in older mice (12–24 months of age). Male C57BL/6J mice raised on a standard chow diet until 12 months of age were assigned to one of three diets: high glycemic, low glycemic, or low glycemic diet containing antibiotics (ampicillin and neomycin) to modify the constituents of the gut microbiome. The group fed the low glycemic diet containing antibiotics showed reductions in whole bone strength that could not be explained by geometry, indicating reduced bone tissue strength (p < 0.007). The high glycemic diet group had larger bone cross-sectional area and moment of inertia and a corresponding greater bone strength as compared to the low glycemic groups, however tissue strength did not noticeably differ from that of the low glycemic group. These findings demonstrate that modifying the gut microbiome in aged mice can alter bone tissue quality. |
| first_indexed | 2024-12-17T01:57:55Z |
| format | Article |
| id | doaj.art-c6cc216833c641e19c6f6e06f6d6f3fe |
| institution | Directory Open Access Journal |
| issn | 2352-1872 |
| language | English |
| last_indexed | 2024-12-17T01:57:55Z |
| publishDate | 2021-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Bone Reports |
| spelling | doaj.art-c6cc216833c641e19c6f6e06f6d6f3fe2022-12-21T22:07:55ZengElsevierBone Reports2352-18722021-06-0114101065Alterations to the gut microbiome impair bone tissue strength in aged miceMacy Castaneda0Kelsey M. Smith1Jacob C. Nixon2Christopher J. Hernandez3Sheldon Rowan4Sibley School of Mechanical and Aerospace Engineering, Cornell University, United States of AmericaJM-USDA Human Nutrition Research Center on Aging, Tufts University, Boston, MA 02111, United States of America; Friedman School of Nutrition and Science Policy, Tufts University, Boston, MA 02111, United States of AmericaSibley School of Mechanical and Aerospace Engineering, Cornell University, United States of AmericaSibley School of Mechanical and Aerospace Engineering, Cornell University, United States of America; Hospital for Special Surgery, New York, NY, United States of America; Correspondence to: C. J Hernandez, 124 Hoy Road, 355 Upson Hall, Ithaca, NY 14850, United States of America.JM-USDA Human Nutrition Research Center on Aging, Tufts University, Boston, MA 02111, United States of America; Friedman School of Nutrition and Science Policy, Tufts University, Boston, MA 02111, United States of America; Correspondence to: S. Rowan, 711 Washington St., Boston, MA 02111, United States of America.Whole bone strength and resistance to fracture are determined by a combination of bone quantity and bone quality – key factors in determining risk for osteoporosis and age-related fractures. Recent preclinical studies have shown that alterations to the gut microbiome can influence bone quantity as well as bone tissue quality. Prior work on the gut microbiome and bone has been limited to young animals, and it is unknown if the gut microbiome can alter bone tissue strength in aged animals. Here we ask if alterations to the constituents of the gut microbiome influence bone strength in older mice (12–24 months of age). Male C57BL/6J mice raised on a standard chow diet until 12 months of age were assigned to one of three diets: high glycemic, low glycemic, or low glycemic diet containing antibiotics (ampicillin and neomycin) to modify the constituents of the gut microbiome. The group fed the low glycemic diet containing antibiotics showed reductions in whole bone strength that could not be explained by geometry, indicating reduced bone tissue strength (p < 0.007). The high glycemic diet group had larger bone cross-sectional area and moment of inertia and a corresponding greater bone strength as compared to the low glycemic groups, however tissue strength did not noticeably differ from that of the low glycemic group. These findings demonstrate that modifying the gut microbiome in aged mice can alter bone tissue quality.http://www.sciencedirect.com/science/article/pii/S235218722100320XAgingBone strengthBone tissue propertiesMicrobiomeBiomechanics |
| spellingShingle | Macy Castaneda Kelsey M. Smith Jacob C. Nixon Christopher J. Hernandez Sheldon Rowan Alterations to the gut microbiome impair bone tissue strength in aged mice Bone Reports Aging Bone strength Bone tissue properties Microbiome Biomechanics |
| title | Alterations to the gut microbiome impair bone tissue strength in aged mice |
| title_full | Alterations to the gut microbiome impair bone tissue strength in aged mice |
| title_fullStr | Alterations to the gut microbiome impair bone tissue strength in aged mice |
| title_full_unstemmed | Alterations to the gut microbiome impair bone tissue strength in aged mice |
| title_short | Alterations to the gut microbiome impair bone tissue strength in aged mice |
| title_sort | alterations to the gut microbiome impair bone tissue strength in aged mice |
| topic | Aging Bone strength Bone tissue properties Microbiome Biomechanics |
| url | http://www.sciencedirect.com/science/article/pii/S235218722100320X |
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