Drug Resistance Determinants in Clinical Isolates of <i>Enterococcus faecalis</i> in Bangladesh: Identification of Oxazolidinone Resistance Gene <i>optrA</i> in ST59 and ST902 Lineages
<i>Enterococcus faecalis</i> is one of the major causes of urinary tract infection, showing acquired resistance to various classes of antimicrobials. The objective of this study was to determine the prevalence of drug resistance and its genetic determinants for <i>E. faecalis</i...
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| Format: | Article |
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MDPI AG
2020-08-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/8/8/1240 |
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| author | Sangjukta Roy Meiji Soe Aung Shyamal Kumar Paul Salma Ahmed Nazia Haque Emily Rahman Khan Tridip Kanti Barman Arup Islam Sahida Abedin Chand Sultana Anindita Paul Muhammad Akram Hossain Noriko Urushibara Mitsuyo Kawaguchiya Ayako Sumi Nobumichi Kobayashi |
| author_facet | Sangjukta Roy Meiji Soe Aung Shyamal Kumar Paul Salma Ahmed Nazia Haque Emily Rahman Khan Tridip Kanti Barman Arup Islam Sahida Abedin Chand Sultana Anindita Paul Muhammad Akram Hossain Noriko Urushibara Mitsuyo Kawaguchiya Ayako Sumi Nobumichi Kobayashi |
| author_sort | Sangjukta Roy |
| collection | DOAJ |
| description | <i>Enterococcus faecalis</i> is one of the major causes of urinary tract infection, showing acquired resistance to various classes of antimicrobials. The objective of this study was to determine the prevalence of drug resistance and its genetic determinants for <i>E. faecalis</i> clinical isolates in north-central Bangladesh. Among a total of 210 <i>E. faecalis</i> isolates, isolated from urine, the resistance rates to erythromycin, levofloxacin, and gentamicin (high level) were 85.2, 45.7, and 11.4%, respectively, while no isolates were resistant to ampicillin, vancomycin and teicoplanin. The most prevalent resistance gene was <i>erm(B)</i> (97%), and any of the four genes encoding aminoglycoside modifying enzyme (AME) were detected in 99 isolates (47%). The AME gene <i>aac(6′)-Ie-aph(2”)-Ia</i> was detected in 46 isolates (21.9%) and was diverse in terms of IS<i>256</i>-flanking patterns, which were associated with resistance level to gentamicin. Tetracycline resistance was ascribable to <i>tet(M)</i> (61%) and <i>tet(L)</i> (38%), and mutations in the quinolone resistance-determining region of both GyrA and ParC were identified in 44% of isolates. Five isolates (2.4%) exhibited non-susceptibility to linezolide (MIC, 4 μg/mL), and harbored the oxazolidinone resistance gene <i>optrA</i>, which was located in a novel genetic cluster containing the phenicol exporter gene <i>fexA</i>. The <i>optrA</i>-positive isolates belonged to ST59, ST902, and ST917 (CC59), while common lineages of other multiple drug-resistant isolates were ST6, ST28, CC16, and CC116. The present study first revealed the prevalence of drug resistance determinants of <i>E. faecalis</i> and their genetic profiles in Bangladesh. |
| first_indexed | 2024-03-10T17:24:44Z |
| format | Article |
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| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T17:24:44Z |
| publishDate | 2020-08-01 |
| publisher | MDPI AG |
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| series | Microorganisms |
| spelling | doaj.art-c6cef28c2f2d4b8cb0c872797d3cd4aa2023-11-20T10:13:42ZengMDPI AGMicroorganisms2076-26072020-08-0188124010.3390/microorganisms8081240Drug Resistance Determinants in Clinical Isolates of <i>Enterococcus faecalis</i> in Bangladesh: Identification of Oxazolidinone Resistance Gene <i>optrA</i> in ST59 and ST902 LineagesSangjukta Roy0Meiji Soe Aung1Shyamal Kumar Paul2Salma Ahmed3Nazia Haque4Emily Rahman Khan5Tridip Kanti Barman6Arup Islam7Sahida Abedin8Chand Sultana9Anindita Paul10Muhammad Akram Hossain11Noriko Urushibara12Mitsuyo Kawaguchiya13Ayako Sumi14Nobumichi Kobayashi15Department of Microbiology, Mymensingh Medical College, Mymensingh 2200, BangladeshDepartment of Hygiene, Sapporo Medical University School of Medicine, Sapporo 060-8556, JapanNetrokona Medical College, Netrokona 2400, BangladeshDepartment of Microbiology, Mymensingh Medical College, Mymensingh 2200, BangladeshDepartment of Microbiology, Mymensingh Medical College, Mymensingh 2200, BangladeshDepartment of Microbiology, Mymensingh Medical College, Mymensingh 2200, BangladeshMedicine Unit, Mymensingh Medical College Hospital, Mymensingh 2200, BangladeshDepartment of Microbiology, Mymensingh Medical College, Mymensingh 2200, BangladeshDepartment of Microbiology, Mymensingh Medical College, Mymensingh 2200, BangladeshDepartment of Microbiology, Mymensingh Medical College, Mymensingh 2200, BangladeshDepartment of Microbiology, Mymensingh Medical College, Mymensingh 2200, BangladeshImperial Hospital Ltd. Chittagong, Chittagong 4202, BangladeshDepartment of Hygiene, Sapporo Medical University School of Medicine, Sapporo 060-8556, JapanDepartment of Hygiene, Sapporo Medical University School of Medicine, Sapporo 060-8556, JapanDepartment of Hygiene, Sapporo Medical University School of Medicine, Sapporo 060-8556, JapanDepartment of Hygiene, Sapporo Medical University School of Medicine, Sapporo 060-8556, Japan<i>Enterococcus faecalis</i> is one of the major causes of urinary tract infection, showing acquired resistance to various classes of antimicrobials. The objective of this study was to determine the prevalence of drug resistance and its genetic determinants for <i>E. faecalis</i> clinical isolates in north-central Bangladesh. Among a total of 210 <i>E. faecalis</i> isolates, isolated from urine, the resistance rates to erythromycin, levofloxacin, and gentamicin (high level) were 85.2, 45.7, and 11.4%, respectively, while no isolates were resistant to ampicillin, vancomycin and teicoplanin. The most prevalent resistance gene was <i>erm(B)</i> (97%), and any of the four genes encoding aminoglycoside modifying enzyme (AME) were detected in 99 isolates (47%). The AME gene <i>aac(6′)-Ie-aph(2”)-Ia</i> was detected in 46 isolates (21.9%) and was diverse in terms of IS<i>256</i>-flanking patterns, which were associated with resistance level to gentamicin. Tetracycline resistance was ascribable to <i>tet(M)</i> (61%) and <i>tet(L)</i> (38%), and mutations in the quinolone resistance-determining region of both GyrA and ParC were identified in 44% of isolates. Five isolates (2.4%) exhibited non-susceptibility to linezolide (MIC, 4 μg/mL), and harbored the oxazolidinone resistance gene <i>optrA</i>, which was located in a novel genetic cluster containing the phenicol exporter gene <i>fexA</i>. The <i>optrA</i>-positive isolates belonged to ST59, ST902, and ST917 (CC59), while common lineages of other multiple drug-resistant isolates were ST6, ST28, CC16, and CC116. The present study first revealed the prevalence of drug resistance determinants of <i>E. faecalis</i> and their genetic profiles in Bangladesh.https://www.mdpi.com/2076-2607/8/8/1240<i>Enterococcus faecalis</i>molecular epidemiologydrug resistance<i>optrA</i>ST |
| spellingShingle | Sangjukta Roy Meiji Soe Aung Shyamal Kumar Paul Salma Ahmed Nazia Haque Emily Rahman Khan Tridip Kanti Barman Arup Islam Sahida Abedin Chand Sultana Anindita Paul Muhammad Akram Hossain Noriko Urushibara Mitsuyo Kawaguchiya Ayako Sumi Nobumichi Kobayashi Drug Resistance Determinants in Clinical Isolates of <i>Enterococcus faecalis</i> in Bangladesh: Identification of Oxazolidinone Resistance Gene <i>optrA</i> in ST59 and ST902 Lineages Microorganisms <i>Enterococcus faecalis</i> molecular epidemiology drug resistance <i>optrA</i> ST |
| title | Drug Resistance Determinants in Clinical Isolates of <i>Enterococcus faecalis</i> in Bangladesh: Identification of Oxazolidinone Resistance Gene <i>optrA</i> in ST59 and ST902 Lineages |
| title_full | Drug Resistance Determinants in Clinical Isolates of <i>Enterococcus faecalis</i> in Bangladesh: Identification of Oxazolidinone Resistance Gene <i>optrA</i> in ST59 and ST902 Lineages |
| title_fullStr | Drug Resistance Determinants in Clinical Isolates of <i>Enterococcus faecalis</i> in Bangladesh: Identification of Oxazolidinone Resistance Gene <i>optrA</i> in ST59 and ST902 Lineages |
| title_full_unstemmed | Drug Resistance Determinants in Clinical Isolates of <i>Enterococcus faecalis</i> in Bangladesh: Identification of Oxazolidinone Resistance Gene <i>optrA</i> in ST59 and ST902 Lineages |
| title_short | Drug Resistance Determinants in Clinical Isolates of <i>Enterococcus faecalis</i> in Bangladesh: Identification of Oxazolidinone Resistance Gene <i>optrA</i> in ST59 and ST902 Lineages |
| title_sort | drug resistance determinants in clinical isolates of i enterococcus faecalis i in bangladesh identification of oxazolidinone resistance gene i optra i in st59 and st902 lineages |
| topic | <i>Enterococcus faecalis</i> molecular epidemiology drug resistance <i>optrA</i> ST |
| url | https://www.mdpi.com/2076-2607/8/8/1240 |
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