New Model of Ventral Spinal Cord Lesion Induced by Balloon Compression in Rats
Despite the variety of experimental models of spinal cord injury (SCI) currently used, the model of the ventral compression cord injury, which is commonly seen in humans, is very limited. Ventral balloon compression injury reflects the common anatomical mechanism of a human lesion and has the advant...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-11-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/8/11/477 |
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| author | Petr Krupa Katerina Stepankova Jessica CF. Kwok James W. Fawcett Veronika Cimermanova Pavla Jendelova Lucia Machova Urdzikova |
| author_facet | Petr Krupa Katerina Stepankova Jessica CF. Kwok James W. Fawcett Veronika Cimermanova Pavla Jendelova Lucia Machova Urdzikova |
| author_sort | Petr Krupa |
| collection | DOAJ |
| description | Despite the variety of experimental models of spinal cord injury (SCI) currently used, the model of the ventral compression cord injury, which is commonly seen in humans, is very limited. Ventral balloon compression injury reflects the common anatomical mechanism of a human lesion and has the advantage of grading the injury severity by controlling the inflated volume of the balloon. In this study, ventral compression of the SCI was performed by the anterior epidural placement of the balloon of a 2F Fogarty’s catheter, via laminectomy, at the level of T10. The balloon was rapidly inflated with 10 or 15 μL of saline and rested in situ for 5 min. The severity of the lesion was assessed by behavioral and immunohistochemical tests. Compression with the volume of 15 μL resulted in severe motor and sensory deficits represented by the complete inability to move across a horizontal ladder, a final Basso, Beattie and Bresnahan (BBB) score of 7.4 and a decreased withdrawal time in the plantar test (11.6 s). Histology and immunohistochemistry revealed a significant loss of white and gray matter with a loss of motoneuron, and an increased size of astrogliosis. An inflation volume of 10 μL resulted in a mild transient deficit. There are no other balloon compression models of ventral spinal cord injury. This study provided and validated a novel, easily replicable model of the ventral compression SCI, introduced by an inflated balloon of Fogarty´s catheter. For a severe incomplete deficit, an inflated volume should be maintained at 15 μL. |
| first_indexed | 2024-03-09T06:04:43Z |
| format | Article |
| id | doaj.art-c6d15584824b4f818076c7e2758b2525 |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-09T06:04:43Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-c6d15584824b4f818076c7e2758b25252023-12-03T12:05:24ZengMDPI AGBiomedicines2227-90592020-11-0181147710.3390/biomedicines8110477New Model of Ventral Spinal Cord Lesion Induced by Balloon Compression in RatsPetr Krupa0Katerina Stepankova1Jessica CF. Kwok2James W. Fawcett3Veronika Cimermanova4Pavla Jendelova5Lucia Machova Urdzikova6Institute of Experimental Medicine, Czech Academy of Sciences, Vídeňská, 1083 Prague, Czech RepublicInstitute of Experimental Medicine, Czech Academy of Sciences, Vídeňská, 1083 Prague, Czech RepublicInstitute of Experimental Medicine, Czech Academy of Sciences, Vídeňská, 1083 Prague, Czech RepublicInstitute of Experimental Medicine, Czech Academy of Sciences, Vídeňská, 1083 Prague, Czech RepublicInstitute of Experimental Medicine, Czech Academy of Sciences, Vídeňská, 1083 Prague, Czech RepublicInstitute of Experimental Medicine, Czech Academy of Sciences, Vídeňská, 1083 Prague, Czech RepublicInstitute of Experimental Medicine, Czech Academy of Sciences, Vídeňská, 1083 Prague, Czech RepublicDespite the variety of experimental models of spinal cord injury (SCI) currently used, the model of the ventral compression cord injury, which is commonly seen in humans, is very limited. Ventral balloon compression injury reflects the common anatomical mechanism of a human lesion and has the advantage of grading the injury severity by controlling the inflated volume of the balloon. In this study, ventral compression of the SCI was performed by the anterior epidural placement of the balloon of a 2F Fogarty’s catheter, via laminectomy, at the level of T10. The balloon was rapidly inflated with 10 or 15 μL of saline and rested in situ for 5 min. The severity of the lesion was assessed by behavioral and immunohistochemical tests. Compression with the volume of 15 μL resulted in severe motor and sensory deficits represented by the complete inability to move across a horizontal ladder, a final Basso, Beattie and Bresnahan (BBB) score of 7.4 and a decreased withdrawal time in the plantar test (11.6 s). Histology and immunohistochemistry revealed a significant loss of white and gray matter with a loss of motoneuron, and an increased size of astrogliosis. An inflation volume of 10 μL resulted in a mild transient deficit. There are no other balloon compression models of ventral spinal cord injury. This study provided and validated a novel, easily replicable model of the ventral compression SCI, introduced by an inflated balloon of Fogarty´s catheter. For a severe incomplete deficit, an inflated volume should be maintained at 15 μL.https://www.mdpi.com/2227-9059/8/11/477ventral spinal cord injuryischemic compression injuryspinal tissue lossastrogliosismotoneurons |
| spellingShingle | Petr Krupa Katerina Stepankova Jessica CF. Kwok James W. Fawcett Veronika Cimermanova Pavla Jendelova Lucia Machova Urdzikova New Model of Ventral Spinal Cord Lesion Induced by Balloon Compression in Rats Biomedicines ventral spinal cord injury ischemic compression injury spinal tissue loss astrogliosis motoneurons |
| title | New Model of Ventral Spinal Cord Lesion Induced by Balloon Compression in Rats |
| title_full | New Model of Ventral Spinal Cord Lesion Induced by Balloon Compression in Rats |
| title_fullStr | New Model of Ventral Spinal Cord Lesion Induced by Balloon Compression in Rats |
| title_full_unstemmed | New Model of Ventral Spinal Cord Lesion Induced by Balloon Compression in Rats |
| title_short | New Model of Ventral Spinal Cord Lesion Induced by Balloon Compression in Rats |
| title_sort | new model of ventral spinal cord lesion induced by balloon compression in rats |
| topic | ventral spinal cord injury ischemic compression injury spinal tissue loss astrogliosis motoneurons |
| url | https://www.mdpi.com/2227-9059/8/11/477 |
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