Effect of Cold Atmospheric Plasma Jet Associated to Polyene Antifungals on <i>Candida albicans</i> Biofilms
The increasing incidence of antifungal resistance represents a great challenge in the medical area and, for this reason, new therapeutic alternatives for the treatment of fungal infections are urgently required. Cold atmospheric plasma (CAP) has been proposed as a promising alternative technique for...
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| Format: | Article |
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MDPI AG
2021-09-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/26/19/5815 |
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| author | Lady Daiane Pereira Leite Maria Alcionéia Carvalho de Oliveira Mariana Raquel da Cruz Vegian Aline da Graça Sampaio Thalita Mayumi Castaldelli Nishime Konstantin Georgiev Kostov Cristiane Yumi Koga-Ito |
| author_facet | Lady Daiane Pereira Leite Maria Alcionéia Carvalho de Oliveira Mariana Raquel da Cruz Vegian Aline da Graça Sampaio Thalita Mayumi Castaldelli Nishime Konstantin Georgiev Kostov Cristiane Yumi Koga-Ito |
| author_sort | Lady Daiane Pereira Leite |
| collection | DOAJ |
| description | The increasing incidence of antifungal resistance represents a great challenge in the medical area and, for this reason, new therapeutic alternatives for the treatment of fungal infections are urgently required. Cold atmospheric plasma (CAP) has been proposed as a promising alternative technique for the treatment of superficial candidiasis, with inhibitory effect both in vitro and in vivo. However, little is known on the association of CAP with conventional antifungals. The aim of this study was to evaluate the effects of the association between CAP and conventional polyene antifungals on <i>Candida albicans</i> biofilms. <i>C. albicans</i> SC 5314 and a clinical isolate were used to grow 24 or 48 h biofilms, under standardized conditions. After that, the biofilms were exposed to nystatin, amphotericin B and CAP, separately or in combination. Different concentrations of the antifungals and sequences of treatment were evaluated to establish the most effective protocol. Biofilms viability after the treatments was compared to negative control. Data were compared by One-way ANOVA and post hoc Tukey (5%). The results demonstrate that 5 min exposure to CAP showed more effective antifungal effect on biofilms when compared to nystatin and amphotericin B. Additionally, it was detected that CAP showed similar (but smaller in magnitude) effects when applied in association with nystatin and amphotericin B at 40 µg/mL and 60 µg/mL. Therefore, it can be concluded that the application of CAP alone was more effective against <i>C. albicans</i> biofilms than in combination with conventional polyene antifungal agents. |
| first_indexed | 2024-03-10T06:55:23Z |
| format | Article |
| id | doaj.art-c6d31d2e8e554f36bf4b21d09e502376 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T06:55:23Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-c6d31d2e8e554f36bf4b21d09e5023762023-11-22T16:33:04ZengMDPI AGMolecules1420-30492021-09-012619581510.3390/molecules26195815Effect of Cold Atmospheric Plasma Jet Associated to Polyene Antifungals on <i>Candida albicans</i> BiofilmsLady Daiane Pereira Leite0Maria Alcionéia Carvalho de Oliveira1Mariana Raquel da Cruz Vegian2Aline da Graça Sampaio3Thalita Mayumi Castaldelli Nishime4Konstantin Georgiev Kostov5Cristiane Yumi Koga-Ito6Science Applied to Oral Health, Graduate Program of Institute of Science and Technology, São Paulo State University (Unesp), São José dos Campos 12245-000, BrazilOral Biopathology, Graduate Program of Institute of Science and Technology, São Paulo State University (Unesp), São José dos Campos 12245-000, BrazilScience Applied to Oral Health, Graduate Program of Institute of Science and Technology, São Paulo State University (Unesp), São José dos Campos 12245-000, BrazilScience Applied to Oral Health, Graduate Program of Institute of Science and Technology, São Paulo State University (Unesp), São José dos Campos 12245-000, BrazilLeibniz Institute for Plasma Science and Technology, 17489 Greifswald, GermanyDepartment of Physics, Guaratinguetá Faculty of Engineering, São Paulo State University (Unesp), Guaratinguetá 12516-410, BrazilScience Applied to Oral Health, Graduate Program of Institute of Science and Technology, São Paulo State University (Unesp), São José dos Campos 12245-000, BrazilThe increasing incidence of antifungal resistance represents a great challenge in the medical area and, for this reason, new therapeutic alternatives for the treatment of fungal infections are urgently required. Cold atmospheric plasma (CAP) has been proposed as a promising alternative technique for the treatment of superficial candidiasis, with inhibitory effect both in vitro and in vivo. However, little is known on the association of CAP with conventional antifungals. The aim of this study was to evaluate the effects of the association between CAP and conventional polyene antifungals on <i>Candida albicans</i> biofilms. <i>C. albicans</i> SC 5314 and a clinical isolate were used to grow 24 or 48 h biofilms, under standardized conditions. After that, the biofilms were exposed to nystatin, amphotericin B and CAP, separately or in combination. Different concentrations of the antifungals and sequences of treatment were evaluated to establish the most effective protocol. Biofilms viability after the treatments was compared to negative control. Data were compared by One-way ANOVA and post hoc Tukey (5%). The results demonstrate that 5 min exposure to CAP showed more effective antifungal effect on biofilms when compared to nystatin and amphotericin B. Additionally, it was detected that CAP showed similar (but smaller in magnitude) effects when applied in association with nystatin and amphotericin B at 40 µg/mL and 60 µg/mL. Therefore, it can be concluded that the application of CAP alone was more effective against <i>C. albicans</i> biofilms than in combination with conventional polyene antifungal agents.https://www.mdpi.com/1420-3049/26/19/5815<i>Candida albicans</i>antifungalcold plasma |
| spellingShingle | Lady Daiane Pereira Leite Maria Alcionéia Carvalho de Oliveira Mariana Raquel da Cruz Vegian Aline da Graça Sampaio Thalita Mayumi Castaldelli Nishime Konstantin Georgiev Kostov Cristiane Yumi Koga-Ito Effect of Cold Atmospheric Plasma Jet Associated to Polyene Antifungals on <i>Candida albicans</i> Biofilms Molecules <i>Candida albicans</i> antifungal cold plasma |
| title | Effect of Cold Atmospheric Plasma Jet Associated to Polyene Antifungals on <i>Candida albicans</i> Biofilms |
| title_full | Effect of Cold Atmospheric Plasma Jet Associated to Polyene Antifungals on <i>Candida albicans</i> Biofilms |
| title_fullStr | Effect of Cold Atmospheric Plasma Jet Associated to Polyene Antifungals on <i>Candida albicans</i> Biofilms |
| title_full_unstemmed | Effect of Cold Atmospheric Plasma Jet Associated to Polyene Antifungals on <i>Candida albicans</i> Biofilms |
| title_short | Effect of Cold Atmospheric Plasma Jet Associated to Polyene Antifungals on <i>Candida albicans</i> Biofilms |
| title_sort | effect of cold atmospheric plasma jet associated to polyene antifungals on i candida albicans i biofilms |
| topic | <i>Candida albicans</i> antifungal cold plasma |
| url | https://www.mdpi.com/1420-3049/26/19/5815 |
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