Green Ultrasound Assisted Extraction of <i>trans</i> Rosmarinic Acid from <i>Plectranthus scutellarioides</i> (L.) R.Br. Leaves
Painted nettle (<i>Plectranthus scutellarioides</i> (L.) R.Br.) is an ornamental plant belonging to <i>Lamiaceae</i> family, native of Asia. Its leaves constitute one of the richest sources of <i>trans</i>-rosmarinic acid, a well-known antioxidant and antimicrobia...
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MDPI AG
2019-02-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/8/3/50 |
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| author | Duangjai Tungmunnithum Laurine Garros Samantha Drouet Sullivan Renouard Eric Lainé Christophe Hano |
| author_facet | Duangjai Tungmunnithum Laurine Garros Samantha Drouet Sullivan Renouard Eric Lainé Christophe Hano |
| author_sort | Duangjai Tungmunnithum |
| collection | DOAJ |
| description | Painted nettle (<i>Plectranthus scutellarioides</i> (L.) R.Br.) is an ornamental plant belonging to <i>Lamiaceae</i> family, native of Asia. Its leaves constitute one of the richest sources of <i>trans</i>-rosmarinic acid, a well-known antioxidant and antimicrobial phenolic compound. These biological activities attract interest from the cosmetic industry and the demand for the development of green sustainable extraction processes. Here, we report on the optimization and validation of an ultrasound-assisted extraction (USAE) method using ethanol as solvent. Following preliminary single factor experiments, the identified limiting extraction parameters (i.e., ultrasound frequency, extraction duration, and ethanol concentration) were further optimized using a full factorial design approach. The method was then validated following the recommendations of the association of analytical communities (AOAC) to ensure the precision and accuracy of the method used to quantify <i>trans</i>-rosmarinic acid. Highest <i>trans</i>-rosmarinic acid content was obtained using pure ethanol as extraction solvent following a 45-minute extraction in an ultrasound bath operating at an ultrasound frequency of 30 kHz. The antioxidant (in vitro radical scavenging activity) and antimicrobial (directed toward <i>Staphylococcus aureus</i> ACTT6538) activities were significantly correlated with the <i>trans</i>-rosmarinic acid concentration of the extract evidencing that these key biological activities were retained following the extraction using this validated method. Under these conditions, 110.8 mg/g DW of <i>trans</i>-rosmarinic acid were extracted from lyophilized <i>P. scutellarioides</i> leaves as starting material evidencing the great potential of this renewable material for cosmetic applications. Comparison to other classical extraction methods evidenced a clear benefit of the present USAE method both in terms of yield and extraction duration. |
| first_indexed | 2024-04-11T16:28:31Z |
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| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-04-11T16:28:31Z |
| publishDate | 2019-02-01 |
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| record_format | Article |
| series | Plants |
| spelling | doaj.art-c6d3e7ac76964d5c8005dfe05ba6b6ef2022-12-22T04:14:07ZengMDPI AGPlants2223-77472019-02-01835010.3390/plants8030050plants8030050Green Ultrasound Assisted Extraction of <i>trans</i> Rosmarinic Acid from <i>Plectranthus scutellarioides</i> (L.) R.Br. LeavesDuangjai Tungmunnithum0Laurine Garros1Samantha Drouet2Sullivan Renouard3Eric Lainé4Christophe Hano5Laboratoire de Biologie des Ligneux et des Grandes Cultures, INRA USC1328, Orleans University, 45067 Orléans Cedex 2, FranceLaboratoire de Biologie des Ligneux et des Grandes Cultures, INRA USC1328, Orleans University, 45067 Orléans Cedex 2, FranceLaboratoire de Biologie des Ligneux et des Grandes Cultures, INRA USC1328, Orleans University, 45067 Orléans Cedex 2, FranceInstitut de Chimie et de Biologie des Membranes et des Nano-objets, CNRS UMR 5248, Bordeaux University, 33600 Pessac, FranceLaboratoire de Biologie des Ligneux et des Grandes Cultures, INRA USC1328, Orleans University, 45067 Orléans Cedex 2, FranceLaboratoire de Biologie des Ligneux et des Grandes Cultures, INRA USC1328, Orleans University, 45067 Orléans Cedex 2, FrancePainted nettle (<i>Plectranthus scutellarioides</i> (L.) R.Br.) is an ornamental plant belonging to <i>Lamiaceae</i> family, native of Asia. Its leaves constitute one of the richest sources of <i>trans</i>-rosmarinic acid, a well-known antioxidant and antimicrobial phenolic compound. These biological activities attract interest from the cosmetic industry and the demand for the development of green sustainable extraction processes. Here, we report on the optimization and validation of an ultrasound-assisted extraction (USAE) method using ethanol as solvent. Following preliminary single factor experiments, the identified limiting extraction parameters (i.e., ultrasound frequency, extraction duration, and ethanol concentration) were further optimized using a full factorial design approach. The method was then validated following the recommendations of the association of analytical communities (AOAC) to ensure the precision and accuracy of the method used to quantify <i>trans</i>-rosmarinic acid. Highest <i>trans</i>-rosmarinic acid content was obtained using pure ethanol as extraction solvent following a 45-minute extraction in an ultrasound bath operating at an ultrasound frequency of 30 kHz. The antioxidant (in vitro radical scavenging activity) and antimicrobial (directed toward <i>Staphylococcus aureus</i> ACTT6538) activities were significantly correlated with the <i>trans</i>-rosmarinic acid concentration of the extract evidencing that these key biological activities were retained following the extraction using this validated method. Under these conditions, 110.8 mg/g DW of <i>trans</i>-rosmarinic acid were extracted from lyophilized <i>P. scutellarioides</i> leaves as starting material evidencing the great potential of this renewable material for cosmetic applications. Comparison to other classical extraction methods evidenced a clear benefit of the present USAE method both in terms of yield and extraction duration.https://www.mdpi.com/2223-7747/8/3/50<i>Plectranthus scutellarioides</i><i>trans</i>-rosmarinic acid<i>Lamiaceae</i>green extractionultrasoundantioxidantantimicrobial |
| spellingShingle | Duangjai Tungmunnithum Laurine Garros Samantha Drouet Sullivan Renouard Eric Lainé Christophe Hano Green Ultrasound Assisted Extraction of <i>trans</i> Rosmarinic Acid from <i>Plectranthus scutellarioides</i> (L.) R.Br. Leaves Plants <i>Plectranthus scutellarioides</i> <i>trans</i>-rosmarinic acid <i>Lamiaceae</i> green extraction ultrasound antioxidant antimicrobial |
| title | Green Ultrasound Assisted Extraction of <i>trans</i> Rosmarinic Acid from <i>Plectranthus scutellarioides</i> (L.) R.Br. Leaves |
| title_full | Green Ultrasound Assisted Extraction of <i>trans</i> Rosmarinic Acid from <i>Plectranthus scutellarioides</i> (L.) R.Br. Leaves |
| title_fullStr | Green Ultrasound Assisted Extraction of <i>trans</i> Rosmarinic Acid from <i>Plectranthus scutellarioides</i> (L.) R.Br. Leaves |
| title_full_unstemmed | Green Ultrasound Assisted Extraction of <i>trans</i> Rosmarinic Acid from <i>Plectranthus scutellarioides</i> (L.) R.Br. Leaves |
| title_short | Green Ultrasound Assisted Extraction of <i>trans</i> Rosmarinic Acid from <i>Plectranthus scutellarioides</i> (L.) R.Br. Leaves |
| title_sort | green ultrasound assisted extraction of i trans i rosmarinic acid from i plectranthus scutellarioides i l r br leaves |
| topic | <i>Plectranthus scutellarioides</i> <i>trans</i>-rosmarinic acid <i>Lamiaceae</i> green extraction ultrasound antioxidant antimicrobial |
| url | https://www.mdpi.com/2223-7747/8/3/50 |
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