Process Optimization and Antioxidant Analysis of Enzymatic Preparation of Hemerocallis citrina Juice
The antioxidant activity of day lily juice before and after enzymatic digestion was investigated by the optimization of the enzymatic digestion process using day lily as raw material. In this study, the enzymatic hydrolysis experiments with different ratios of cellulase and pectinase were carried ou...
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| Format: | Article |
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The editorial department of Science and Technology of Food Industry
2023-06-01
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| Series: | Shipin gongye ke-ji |
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| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022080058 |
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| author | Wentao GAO Yibin JIA Yanbin WEN Gongjing CHAI Jingming LI |
| author_facet | Wentao GAO Yibin JIA Yanbin WEN Gongjing CHAI Jingming LI |
| author_sort | Wentao GAO |
| collection | DOAJ |
| description | The antioxidant activity of day lily juice before and after enzymatic digestion was investigated by the optimization of the enzymatic digestion process using day lily as raw material. In this study, the enzymatic hydrolysis experiments with different ratios of cellulase and pectinase were carried out, and the best enzymatic digestion process of day lily juice was determined by response surface optimization using the juice yield of day lily as an index. The polyphenols in the samples were analyzed qualitatively and quantitatively by high performance liquid chromatography-triple quadrupole mass spectrometry, and the antioxidant activity of the samples in vitro was also evaluated. The optimal enzymolysis conditions were obtained as follows：Enzymatic digestion temperature at 50 ℃ and time of 2 h, the total enzyme addition of 0.4% per 100 g of day lily juice (1:1 ratio of day lily to water), and cellulase:pectinase=1:3 , and the juice yield was 78.54%. The total phenol content increased by 1.3 times, the DPPH free radical scavenging rate increased by 9.02%, and the ABTS+ free radical scavenging rate increased by 6.15%. The correlation coefficient between total phenol and DPPH free radical scavenging capacity was 0.929, and the correlation coefficient between total phenol and ABTS+ free radical scavenging capacity was 0.968, indicating that the antioxidant capacity in vitro of day lily juice was closely related to the change of total phenol content. |
| first_indexed | 2024-03-12T13:34:43Z |
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| institution | Directory Open Access Journal |
| issn | 1002-0306 |
| language | zho |
| last_indexed | 2024-03-12T13:34:43Z |
| publishDate | 2023-06-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
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| series | Shipin gongye ke-ji |
| spelling | doaj.art-c6d4594a7282465aa91e5f3c7cbf12202023-08-24T06:00:59ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062023-06-01441216417110.13386/j.issn1002-0306.20220800582022080058-12Process Optimization and Antioxidant Analysis of Enzymatic Preparation of Hemerocallis citrina JuiceWentao GAO0Yibin JIA1Yanbin WEN2Gongjing CHAI3Jingming LI4School of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, ChinaSchool of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, ChinaDatong Municipal Bureau of Agriculture, Datong 037006, ChinaTianjin Dongli District Agricultural and Rural Development Service Center, Tianjin 300300, ChinaSchool of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, ChinaThe antioxidant activity of day lily juice before and after enzymatic digestion was investigated by the optimization of the enzymatic digestion process using day lily as raw material. In this study, the enzymatic hydrolysis experiments with different ratios of cellulase and pectinase were carried out, and the best enzymatic digestion process of day lily juice was determined by response surface optimization using the juice yield of day lily as an index. The polyphenols in the samples were analyzed qualitatively and quantitatively by high performance liquid chromatography-triple quadrupole mass spectrometry, and the antioxidant activity of the samples in vitro was also evaluated. The optimal enzymolysis conditions were obtained as follows：Enzymatic digestion temperature at 50 ℃ and time of 2 h, the total enzyme addition of 0.4% per 100 g of day lily juice (1:1 ratio of day lily to water), and cellulase:pectinase=1:3 , and the juice yield was 78.54%. The total phenol content increased by 1.3 times, the DPPH free radical scavenging rate increased by 9.02%, and the ABTS+ free radical scavenging rate increased by 6.15%. The correlation coefficient between total phenol and DPPH free radical scavenging capacity was 0.929, and the correlation coefficient between total phenol and ABTS+ free radical scavenging capacity was 0.968, indicating that the antioxidant capacity in vitro of day lily juice was closely related to the change of total phenol content.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022080058day lilyenzymatic hydrolysisjuicinghigh performance liquid chromatography-mass spectrometrypolyphenolsoxidation resistance |
| spellingShingle | Wentao GAO Yibin JIA Yanbin WEN Gongjing CHAI Jingming LI Process Optimization and Antioxidant Analysis of Enzymatic Preparation of Hemerocallis citrina Juice Shipin gongye ke-ji day lily enzymatic hydrolysis juicing high performance liquid chromatography-mass spectrometry polyphenols oxidation resistance |
| title | Process Optimization and Antioxidant Analysis of Enzymatic Preparation of Hemerocallis citrina Juice |
| title_full | Process Optimization and Antioxidant Analysis of Enzymatic Preparation of Hemerocallis citrina Juice |
| title_fullStr | Process Optimization and Antioxidant Analysis of Enzymatic Preparation of Hemerocallis citrina Juice |
| title_full_unstemmed | Process Optimization and Antioxidant Analysis of Enzymatic Preparation of Hemerocallis citrina Juice |
| title_short | Process Optimization and Antioxidant Analysis of Enzymatic Preparation of Hemerocallis citrina Juice |
| title_sort | process optimization and antioxidant analysis of enzymatic preparation of hemerocallis citrina juice |
| topic | day lily enzymatic hydrolysis juicing high performance liquid chromatography-mass spectrometry polyphenols oxidation resistance |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022080058 |
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