Propofol Suppresses Gastric Cancer Progression by Regulating circPDSS1/miR-1324/SOX4 Axis
Leyi Liu,1 Ting Dong,2 Jun Sheng3 1Department of Anesthesiology, Wuhu Hospital of Traditional Chinese Medicine, Wuhu, 241000, People’s Republic of China; 2Yijishan Hospital, First Affiliated Hospital of Wannan Medical College, Wuhu, 241001, People’s Republic of China; 3Department of Oncology, Wuhu H...
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Dove Medical Press
2021-08-01
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| Series: | Cancer Management and Research |
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| Online Access: | https://www.dovepress.com/propofol-suppresses-gastric-cancer-progression-by-regulating-circpdss1-peer-reviewed-fulltext-article-CMAR |
| _version_ | 1819035479534731264 |
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| author | Liu L Dong T Sheng J |
| author_facet | Liu L Dong T Sheng J |
| author_sort | Liu L |
| collection | DOAJ |
| description | Leyi Liu,1 Ting Dong,2 Jun Sheng3 1Department of Anesthesiology, Wuhu Hospital of Traditional Chinese Medicine, Wuhu, 241000, People’s Republic of China; 2Yijishan Hospital, First Affiliated Hospital of Wannan Medical College, Wuhu, 241001, People’s Republic of China; 3Department of Oncology, Wuhu Hospital of Traditional Chinese Medicine, Wuhu, 241000, People’s Republic of ChinaCorrespondence: Jun ShengDepartment of Oncology, Wuhu Hospital of Traditional Chinese Medicine, No. 430, Jiuhua South Road, Yijiang District, Wuhu, 241000, People’s Republic of ChinaEmail sj6785@126.comBackground: Propofol is a common intravenous anesthetic that exerts an antitumor role in human cancers. Circular RNAs (circRNAs) play crucial roles in the progression of various cancers. However, the relationship between propofol and circRNA decaprenyl diphosphate synthase subunit 1 (circPDSS1) in gastric cancer (GC) remains unclear.Methods: Cell proliferation was evaluated by Cell Counting Kit-8 (CCK-8), colony formation, and 5-ethynyl-2ʹ-deoxyuridine (EdU) assays. Cell migration and invasion were assessed by transwell assay. Cell apoptosis was determined by flow cytometry. All protein levels were detected by Western blot assay. The expression levels of circPDSS1, microRNA-1324 (miR-1324), and SRY-box transcription factor 4 (SOX4) mRNA were determined by quantitative real-time PCR (qRT-PCR). The interaction between miR-1324 and circPDSS1 or SOX4 was confirmed by dual-luciferase reporter and RNA pull-down assays. The mice xenograft model was established to investigate the role of propofol and circPDSS1 in vivo.Results: Propofol inhibited cell proliferation, migration and invasion and induced apoptosis in GC cells, which could be reversed by upregulating circPDSS1. MiR-1324 was a target of circPDSS1, and circPDSS1 promoted cell proliferation, migration and invasion and reduced apoptosis in propofol-treated cells by sponging miR-1324. Moreover, SOX4 was a direct target of miR-1324, and miR-1324 exerted anticancer role by targeting SOX4 in propofol-treated cells. CircPDSS1 acted as a sponge of miR-1324 to regulate SOX4 expression. Additionally, circPDSS1 overexpression weakened the anticancer role of propofol in vivo.Conclusion: Propofol exerted anticancer role in GC through regulating circPDSS1/miR-1324/SOX4 axis, indicating that propofol might be an effective therapeutic medicine for GC treatment.Keywords: gastric cancer, propofol, circPDSS1, miR-1324, SOX4 |
| first_indexed | 2024-12-21T07:50:17Z |
| format | Article |
| id | doaj.art-c6d47b5debaf45059108fcbbdf77e08d |
| institution | Directory Open Access Journal |
| issn | 1179-1322 |
| language | English |
| last_indexed | 2024-12-21T07:50:17Z |
| publishDate | 2021-08-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Cancer Management and Research |
| spelling | doaj.art-c6d47b5debaf45059108fcbbdf77e08d2022-12-21T19:11:05ZengDove Medical PressCancer Management and Research1179-13222021-08-01Volume 136031604367501Propofol Suppresses Gastric Cancer Progression by Regulating circPDSS1/miR-1324/SOX4 AxisLiu LDong TSheng JLeyi Liu,1 Ting Dong,2 Jun Sheng3 1Department of Anesthesiology, Wuhu Hospital of Traditional Chinese Medicine, Wuhu, 241000, People’s Republic of China; 2Yijishan Hospital, First Affiliated Hospital of Wannan Medical College, Wuhu, 241001, People’s Republic of China; 3Department of Oncology, Wuhu Hospital of Traditional Chinese Medicine, Wuhu, 241000, People’s Republic of ChinaCorrespondence: Jun ShengDepartment of Oncology, Wuhu Hospital of Traditional Chinese Medicine, No. 430, Jiuhua South Road, Yijiang District, Wuhu, 241000, People’s Republic of ChinaEmail sj6785@126.comBackground: Propofol is a common intravenous anesthetic that exerts an antitumor role in human cancers. Circular RNAs (circRNAs) play crucial roles in the progression of various cancers. However, the relationship between propofol and circRNA decaprenyl diphosphate synthase subunit 1 (circPDSS1) in gastric cancer (GC) remains unclear.Methods: Cell proliferation was evaluated by Cell Counting Kit-8 (CCK-8), colony formation, and 5-ethynyl-2ʹ-deoxyuridine (EdU) assays. Cell migration and invasion were assessed by transwell assay. Cell apoptosis was determined by flow cytometry. All protein levels were detected by Western blot assay. The expression levels of circPDSS1, microRNA-1324 (miR-1324), and SRY-box transcription factor 4 (SOX4) mRNA were determined by quantitative real-time PCR (qRT-PCR). The interaction between miR-1324 and circPDSS1 or SOX4 was confirmed by dual-luciferase reporter and RNA pull-down assays. The mice xenograft model was established to investigate the role of propofol and circPDSS1 in vivo.Results: Propofol inhibited cell proliferation, migration and invasion and induced apoptosis in GC cells, which could be reversed by upregulating circPDSS1. MiR-1324 was a target of circPDSS1, and circPDSS1 promoted cell proliferation, migration and invasion and reduced apoptosis in propofol-treated cells by sponging miR-1324. Moreover, SOX4 was a direct target of miR-1324, and miR-1324 exerted anticancer role by targeting SOX4 in propofol-treated cells. CircPDSS1 acted as a sponge of miR-1324 to regulate SOX4 expression. Additionally, circPDSS1 overexpression weakened the anticancer role of propofol in vivo.Conclusion: Propofol exerted anticancer role in GC through regulating circPDSS1/miR-1324/SOX4 axis, indicating that propofol might be an effective therapeutic medicine for GC treatment.Keywords: gastric cancer, propofol, circPDSS1, miR-1324, SOX4https://www.dovepress.com/propofol-suppresses-gastric-cancer-progression-by-regulating-circpdss1-peer-reviewed-fulltext-article-CMARgastric cancerpropofolcircpdss1mir-1324sox4 |
| spellingShingle | Liu L Dong T Sheng J Propofol Suppresses Gastric Cancer Progression by Regulating circPDSS1/miR-1324/SOX4 Axis Cancer Management and Research gastric cancer propofol circpdss1 mir-1324 sox4 |
| title | Propofol Suppresses Gastric Cancer Progression by Regulating circPDSS1/miR-1324/SOX4 Axis |
| title_full | Propofol Suppresses Gastric Cancer Progression by Regulating circPDSS1/miR-1324/SOX4 Axis |
| title_fullStr | Propofol Suppresses Gastric Cancer Progression by Regulating circPDSS1/miR-1324/SOX4 Axis |
| title_full_unstemmed | Propofol Suppresses Gastric Cancer Progression by Regulating circPDSS1/miR-1324/SOX4 Axis |
| title_short | Propofol Suppresses Gastric Cancer Progression by Regulating circPDSS1/miR-1324/SOX4 Axis |
| title_sort | propofol suppresses gastric cancer progression by regulating circpdss1 mir 1324 sox4 axis |
| topic | gastric cancer propofol circpdss1 mir-1324 sox4 |
| url | https://www.dovepress.com/propofol-suppresses-gastric-cancer-progression-by-regulating-circpdss1-peer-reviewed-fulltext-article-CMAR |
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