Novel transgenic pigs with enhanced growth and reduced environmental impact
In pig production, inefficient feed digestion causes excessive nutrients such as phosphorus and nitrogen to be released to the environment. To address the issue of environmental emissions, we established transgenic pigs harboring a single-copy quad-cistronic transgene and simultaneously expressing t...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2018-05-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/34286 |
| _version_ | 1811253030704644096 |
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| author | Xianwei Zhang Zicong Li Huaqiang Yang Dewu Liu Gengyuan Cai Guoling Li Jianxin Mo Dehua Wang Cuili Zhong Haoqiang Wang Yue Sun Junsong Shi Enqin Zheng Fanming Meng Mao Zhang Xiaoyan He Rong Zhou Jian Zhang Miaorong Huang Ran Zhang Ning Li Mingzhe Fan Jinzeng Yang Zhenfang Wu |
| author_facet | Xianwei Zhang Zicong Li Huaqiang Yang Dewu Liu Gengyuan Cai Guoling Li Jianxin Mo Dehua Wang Cuili Zhong Haoqiang Wang Yue Sun Junsong Shi Enqin Zheng Fanming Meng Mao Zhang Xiaoyan He Rong Zhou Jian Zhang Miaorong Huang Ran Zhang Ning Li Mingzhe Fan Jinzeng Yang Zhenfang Wu |
| author_sort | Xianwei Zhang |
| collection | DOAJ |
| description | In pig production, inefficient feed digestion causes excessive nutrients such as phosphorus and nitrogen to be released to the environment. To address the issue of environmental emissions, we established transgenic pigs harboring a single-copy quad-cistronic transgene and simultaneously expressing three microbial enzymes, β-glucanase, xylanase, and phytase in the salivary glands. All the transgenic enzymes were successfully expressed, and the digestion of non-starch polysaccharides (NSPs) and phytate in the feedstuff was enhanced. Fecal nitrogen and phosphorus outputs in the transgenic pigs were reduced by 23.2–45.8%, and growth rate improved by 23.0% (gilts) and 24.4% (boars) compared with that of age-matched wild-type littermates under the same dietary treatment. The transgenic pigs showed an 11.5–14.5% improvement in feed conversion rate compared with the wild-type pigs. These findings indicate that the transgenic pigs are promising resources for improving feed efficiency and reducing environmental impact. |
| first_indexed | 2024-04-12T16:44:35Z |
| format | Article |
| id | doaj.art-c6d4ddbac66a4bf6821d2d76c49a419f |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T16:44:35Z |
| publishDate | 2018-05-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-c6d4ddbac66a4bf6821d2d76c49a419f2022-12-22T03:24:39ZengeLife Sciences Publications LtdeLife2050-084X2018-05-01710.7554/eLife.34286Novel transgenic pigs with enhanced growth and reduced environmental impactXianwei Zhang0https://orcid.org/0000-0001-6205-3050Zicong Li1https://orcid.org/0000-0002-6997-4669Huaqiang Yang2https://orcid.org/0000-0002-4287-0026Dewu Liu3Gengyuan Cai4Guoling Li5Jianxin Mo6Dehua Wang7Cuili Zhong8Haoqiang Wang9Yue Sun10Junsong Shi11Enqin Zheng12Fanming Meng13Mao Zhang14Xiaoyan He15Rong Zhou16Jian Zhang17Miaorong Huang18Ran Zhang19Ning Li20Mingzhe Fan21Jinzeng Yang22Zhenfang Wu23https://orcid.org/0000-0002-5586-6771College of Animal Science, South China Agricultural University, Guangzhou, China; National Engineering Research Center for Breeding Swine Industry, Guangdong Wens Foodstuff Group Co., Ltd, Yunfu, ChinaCollege of Animal Science, South China Agricultural University, Guangzhou, ChinaCollege of Animal Science, South China Agricultural University, Guangzhou, China; National Engineering Research Center for Breeding Swine Industry, Guangdong Wens Foodstuff Group Co., Ltd, Yunfu, ChinaCollege of Animal Science, South China Agricultural University, Guangzhou, ChinaCollege of Animal Science, South China Agricultural University, Guangzhou, China; National Engineering Research Center for Breeding Swine Industry, Guangdong Wens Foodstuff Group Co., Ltd, Yunfu, ChinaCollege of Animal Science, South China Agricultural University, Guangzhou, ChinaCollege of Animal Science, South China Agricultural University, Guangzhou, ChinaCollege of Animal Science, South China Agricultural University, Guangzhou, ChinaCollege of Animal Science, South China Agricultural University, Guangzhou, ChinaCollege of Animal Science, South China Agricultural University, Guangzhou, ChinaCollege of Animal Science, South China Agricultural University, Guangzhou, ChinaNational Engineering Research Center for Breeding Swine Industry, Guangdong Wens Foodstuff Group Co., Ltd, Yunfu, ChinaCollege of Animal Science, South China Agricultural University, Guangzhou, ChinaCollege of Animal Science, South China Agricultural University, Guangzhou, ChinaCollege of Animal Science, South China Agricultural University, Guangzhou, ChinaCollege of Animal Science, South China Agricultural University, Guangzhou, China; National Engineering Research Center for Breeding Swine Industry, Guangdong Wens Foodstuff Group Co., Ltd, Yunfu, ChinaNational Engineering Research Center for Breeding Swine Industry, Guangdong Wens Foodstuff Group Co., Ltd, Yunfu, ChinaNational Engineering Research Center for Breeding Swine Industry, Guangdong Wens Foodstuff Group Co., Ltd, Yunfu, ChinaCollege of Animal Science, South China Agricultural University, Guangzhou, ChinaCollege of Biological Science, China Agricultural University, Beijing, ChinaCollege of Biological Science, China Agricultural University, Beijing, ChinaDepartment of Animal Biosciences, University of Guelph, Guelph, CanadaDepartment of Human Nutrition, Food and Animal Sciences, University of Hawaii at Manoa, Honolulu, United StatesCollege of Animal Science, South China Agricultural University, Guangzhou, China; National Engineering Research Center for Breeding Swine Industry, Guangdong Wens Foodstuff Group Co., Ltd, Yunfu, ChinaIn pig production, inefficient feed digestion causes excessive nutrients such as phosphorus and nitrogen to be released to the environment. To address the issue of environmental emissions, we established transgenic pigs harboring a single-copy quad-cistronic transgene and simultaneously expressing three microbial enzymes, β-glucanase, xylanase, and phytase in the salivary glands. All the transgenic enzymes were successfully expressed, and the digestion of non-starch polysaccharides (NSPs) and phytate in the feedstuff was enhanced. Fecal nitrogen and phosphorus outputs in the transgenic pigs were reduced by 23.2–45.8%, and growth rate improved by 23.0% (gilts) and 24.4% (boars) compared with that of age-matched wild-type littermates under the same dietary treatment. The transgenic pigs showed an 11.5–14.5% improvement in feed conversion rate compared with the wild-type pigs. These findings indicate that the transgenic pigs are promising resources for improving feed efficiency and reducing environmental impact.https://elifesciences.org/articles/34286Transgenic pigsSalivary glandsNitrogenPhosphorusNonstarch polysaccharides |
| spellingShingle | Xianwei Zhang Zicong Li Huaqiang Yang Dewu Liu Gengyuan Cai Guoling Li Jianxin Mo Dehua Wang Cuili Zhong Haoqiang Wang Yue Sun Junsong Shi Enqin Zheng Fanming Meng Mao Zhang Xiaoyan He Rong Zhou Jian Zhang Miaorong Huang Ran Zhang Ning Li Mingzhe Fan Jinzeng Yang Zhenfang Wu Novel transgenic pigs with enhanced growth and reduced environmental impact eLife Transgenic pigs Salivary glands Nitrogen Phosphorus Nonstarch polysaccharides |
| title | Novel transgenic pigs with enhanced growth and reduced environmental impact |
| title_full | Novel transgenic pigs with enhanced growth and reduced environmental impact |
| title_fullStr | Novel transgenic pigs with enhanced growth and reduced environmental impact |
| title_full_unstemmed | Novel transgenic pigs with enhanced growth and reduced environmental impact |
| title_short | Novel transgenic pigs with enhanced growth and reduced environmental impact |
| title_sort | novel transgenic pigs with enhanced growth and reduced environmental impact |
| topic | Transgenic pigs Salivary glands Nitrogen Phosphorus Nonstarch polysaccharides |
| url | https://elifesciences.org/articles/34286 |
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