Effect of dietary supplements in American bullfrogs reared in low and high stocking densities
The aim of this study was to evaluate the effect of the probiotic Bacillus subtillis and beta-glucan from the fungus Agaricus blazei on survival, growth and immunological capacity in bullfrogs (Lithobates catesbeianus) cultured in low and high stocking densities. Animals weighing 24.3 ± 2.38 g were...
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| Format: | Article |
| Language: | English |
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Elsevier
2017-11-01
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| Series: | Aquaculture Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513417300091 |
| _version_ | 1818311359443501056 |
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| author | Jorgina Juliana Gradisse Freitas Priscila Viau Claudio Alvarenga Oliviera Patricia Coelho Teixeira Leonardo Tachibana Danielle de Carla Dias Marcio Hipolito Isabella Cristina C. Bordon Sthefany Rosa Alfaia Claudia Maris Ferreira |
| author_facet | Jorgina Juliana Gradisse Freitas Priscila Viau Claudio Alvarenga Oliviera Patricia Coelho Teixeira Leonardo Tachibana Danielle de Carla Dias Marcio Hipolito Isabella Cristina C. Bordon Sthefany Rosa Alfaia Claudia Maris Ferreira |
| author_sort | Jorgina Juliana Gradisse Freitas |
| collection | DOAJ |
| description | The aim of this study was to evaluate the effect of the probiotic Bacillus subtillis and beta-glucan from the fungus Agaricus blazei on survival, growth and immunological capacity in bullfrogs (Lithobates catesbeianus) cultured in low and high stocking densities. Animals weighing 24.3 ± 2.38 g were randomly distributed into four treatments with four simultaneous replicates: D100: 100 frogs/m2 (control); D236: 236 frogs/m2; D236 + Prob.: 236 frogs/m2 supplemented with probiotic; and D236 + BG: 236 frogs/m2 supplemented with beta-glucan. The parameters evaluated were weight gain, survival, plasma corticosterone (CORT), phagocytic capacity (PC) and phagocytic index (PI), at 24 h and 15 and 30 days. There is significant interaction between treatments and time for CORT levels. At 30 days, these values were very close for the D100 (control) and D236 + BG groups. Meanwhile, no statistical differences were observed between treatments for PC and PI. These results indicate that beta-glucan reduced the effects of stress caused by high density in bullfrogs, but the probiotic did not reduce these effects. Both compounds are not efficient at increasing survival rates, weight gain and neither immune response of animals. Thus, the use of commercial food additives may not have the favorable impact desired by the farmer. Their use in aquaculture should be further studied in experiments involving a longer trial period and taking into account the cost of their use. |
| first_indexed | 2024-12-13T08:00:42Z |
| format | Article |
| id | doaj.art-c6d82577bd3446c1b6656301f3d1f4f8 |
| institution | Directory Open Access Journal |
| issn | 2352-5134 |
| language | English |
| last_indexed | 2024-12-13T08:00:42Z |
| publishDate | 2017-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj.art-c6d82577bd3446c1b6656301f3d1f4f82022-12-21T23:54:27ZengElsevierAquaculture Reports2352-51342017-11-018C454810.1016/j.aqrep.2017.09.003Effect of dietary supplements in American bullfrogs reared in low and high stocking densitiesJorgina Juliana Gradisse Freitas0Priscila Viau1Claudio Alvarenga Oliviera2Patricia Coelho Teixeira3Leonardo Tachibana4Danielle de Carla Dias5Marcio Hipolito6Isabella Cristina C. Bordon7Sthefany Rosa Alfaia8Claudia Maris Ferreira9Fisheries Institute, APTA-SAA, Av. Francisco Matarazzo Avenue, 455, São Paulo, SP, CEP 05001-970, BrazilDepartment of Animal Reproduction at University of São Paulo’s School of Veterinary Medicine and Animal Science (FMVZ-USP), Av. Prof. Dr. Orlando Marques de Paiva, 87, CEP 05508-270, BrazilDepartment of Animal Reproduction at University of São Paulo’s School of Veterinary Medicine and Animal Science (FMVZ-USP), Av. Prof. Dr. Orlando Marques de Paiva, 87, CEP 05508-270, BrazilFisheries Institute, APTA-SAA, Av. Francisco Matarazzo Avenue, 455, São Paulo, SP, CEP 05001-970, BrazilFisheries Institute, APTA-SAA, Av. Francisco Matarazzo Avenue, 455, São Paulo, SP, CEP 05001-970, BrazilFisheries Institute, APTA-SAA, Av. Francisco Matarazzo Avenue, 455, São Paulo, SP, CEP 05001-970, BrazilBiological Institute, APTA-SAA, Av. Conselheiro Rodrigues Alves, 1252, São Paulo, SP, CEP 04014-002, BrazilUniv. Estadual Paulista (UNESP), Instituto de Biociências, Campus do Litoral Paulista, Núcleo de Estudos em Poluição e Ecotoxicologia Aquática (NEPEA), Praça Infante Dom Henrique, s/n, São Vicente, SP, CEP 11330-900, BrazilFisheries Institute, APTA-SAA, Av. Francisco Matarazzo Avenue, 455, São Paulo, SP, CEP 05001-970, BrazilFisheries Institute, APTA-SAA, Av. Francisco Matarazzo Avenue, 455, São Paulo, SP, CEP 05001-970, BrazilThe aim of this study was to evaluate the effect of the probiotic Bacillus subtillis and beta-glucan from the fungus Agaricus blazei on survival, growth and immunological capacity in bullfrogs (Lithobates catesbeianus) cultured in low and high stocking densities. Animals weighing 24.3 ± 2.38 g were randomly distributed into four treatments with four simultaneous replicates: D100: 100 frogs/m2 (control); D236: 236 frogs/m2; D236 + Prob.: 236 frogs/m2 supplemented with probiotic; and D236 + BG: 236 frogs/m2 supplemented with beta-glucan. The parameters evaluated were weight gain, survival, plasma corticosterone (CORT), phagocytic capacity (PC) and phagocytic index (PI), at 24 h and 15 and 30 days. There is significant interaction between treatments and time for CORT levels. At 30 days, these values were very close for the D100 (control) and D236 + BG groups. Meanwhile, no statistical differences were observed between treatments for PC and PI. These results indicate that beta-glucan reduced the effects of stress caused by high density in bullfrogs, but the probiotic did not reduce these effects. Both compounds are not efficient at increasing survival rates, weight gain and neither immune response of animals. Thus, the use of commercial food additives may not have the favorable impact desired by the farmer. Their use in aquaculture should be further studied in experiments involving a longer trial period and taking into account the cost of their use.http://www.sciencedirect.com/science/article/pii/S2352513417300091CorticosteroneLithobates catesbeianusImmune responseProbioticBeta-glucan |
| spellingShingle | Jorgina Juliana Gradisse Freitas Priscila Viau Claudio Alvarenga Oliviera Patricia Coelho Teixeira Leonardo Tachibana Danielle de Carla Dias Marcio Hipolito Isabella Cristina C. Bordon Sthefany Rosa Alfaia Claudia Maris Ferreira Effect of dietary supplements in American bullfrogs reared in low and high stocking densities Aquaculture Reports Corticosterone Lithobates catesbeianus Immune response Probiotic Beta-glucan |
| title | Effect of dietary supplements in American bullfrogs reared in low and high stocking densities |
| title_full | Effect of dietary supplements in American bullfrogs reared in low and high stocking densities |
| title_fullStr | Effect of dietary supplements in American bullfrogs reared in low and high stocking densities |
| title_full_unstemmed | Effect of dietary supplements in American bullfrogs reared in low and high stocking densities |
| title_short | Effect of dietary supplements in American bullfrogs reared in low and high stocking densities |
| title_sort | effect of dietary supplements in american bullfrogs reared in low and high stocking densities |
| topic | Corticosterone Lithobates catesbeianus Immune response Probiotic Beta-glucan |
| url | http://www.sciencedirect.com/science/article/pii/S2352513417300091 |
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