Determining the SARS-CoV-2 Anti-Spike Cutoff Level Denoting Neutralizing Activity Using Two Commercial Kits
Background: The viral neutralization assay is the gold standard to estimate the level of immunity against SARS-CoV-2. This study analyzes the correlation between the quantitative Anti-SARS-CoV-2 QuantiVac ELISA (IgG) and the NeutraLISA neutralization assay. Methods: 650 serum samples were tested for...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-11-01
|
| Series: | Vaccines |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-393X/10/11/1952 |
| _version_ | 1797463844906336256 |
|---|---|
| author | Engy Mohamed El-Ghitany Mona H. Hashish Azza Galal Farghaly Eman A. Omran |
| author_facet | Engy Mohamed El-Ghitany Mona H. Hashish Azza Galal Farghaly Eman A. Omran |
| author_sort | Engy Mohamed El-Ghitany |
| collection | DOAJ |
| description | Background: The viral neutralization assay is the gold standard to estimate the level of immunity against SARS-CoV-2. This study analyzes the correlation between the quantitative Anti-SARS-CoV-2 QuantiVac ELISA (IgG) and the NeutraLISA neutralization assay. Methods: 650 serum samples were tested for both SARS-CoV-2 anti-spike (anti-S) immunoglobulin G (IgG) and neutralizing antibodies (nAbs) using kits by EUROIMMUN, Germany. Results: There was a significant correlation between levels of anti-S and nAbs (Spearman’s rho = 0.913). Among the positive samples for anti-S, 77.0% (n = 345) were positive for nAbs. There was a substantial agreement between anti-S and nAbs (Cohen’s kappa coefficient = 0.658; agreement of 83.38%). Considering NeutraLISA as a gold standard, anti-S had a sensitivity of 98.57%, specificity of 65.66%, NPV of 97.5%, and PPV of 77.0%. When the anti-S titer was greater than 18.1 RU/mL (57.9 BAU/mL), nAbs were positive, with a sensitivity of 90.0% and specificity of 91%. Conclusions: A titer of SARS-CoV-2 anti-S IgG can be correlated with levels of nAbs. |
| first_indexed | 2024-03-09T17:56:32Z |
| format | Article |
| id | doaj.art-c6dbd0bb6ba94c5987fc3008b802aa82 |
| institution | Directory Open Access Journal |
| issn | 2076-393X |
| language | English |
| last_indexed | 2024-03-09T17:56:32Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Vaccines |
| spelling | doaj.art-c6dbd0bb6ba94c5987fc3008b802aa822023-11-24T10:17:11ZengMDPI AGVaccines2076-393X2022-11-011011195210.3390/vaccines10111952Determining the SARS-CoV-2 Anti-Spike Cutoff Level Denoting Neutralizing Activity Using Two Commercial KitsEngy Mohamed El-Ghitany0Mona H. Hashish1Azza Galal Farghaly2Eman A. Omran3Department of Tropical Health, High Institute of Public Health, Alexandria University, Alexandria 21526, EgyptDepartment of Microbiology, High Institute of Public Health, Alexandria University, Alexandria 21526, EgyptDepartment of Tropical Health, High Institute of Public Health, Alexandria University, Alexandria 21526, EgyptDepartment of Microbiology, High Institute of Public Health, Alexandria University, Alexandria 21526, EgyptBackground: The viral neutralization assay is the gold standard to estimate the level of immunity against SARS-CoV-2. This study analyzes the correlation between the quantitative Anti-SARS-CoV-2 QuantiVac ELISA (IgG) and the NeutraLISA neutralization assay. Methods: 650 serum samples were tested for both SARS-CoV-2 anti-spike (anti-S) immunoglobulin G (IgG) and neutralizing antibodies (nAbs) using kits by EUROIMMUN, Germany. Results: There was a significant correlation between levels of anti-S and nAbs (Spearman’s rho = 0.913). Among the positive samples for anti-S, 77.0% (n = 345) were positive for nAbs. There was a substantial agreement between anti-S and nAbs (Cohen’s kappa coefficient = 0.658; agreement of 83.38%). Considering NeutraLISA as a gold standard, anti-S had a sensitivity of 98.57%, specificity of 65.66%, NPV of 97.5%, and PPV of 77.0%. When the anti-S titer was greater than 18.1 RU/mL (57.9 BAU/mL), nAbs were positive, with a sensitivity of 90.0% and specificity of 91%. Conclusions: A titer of SARS-CoV-2 anti-S IgG can be correlated with levels of nAbs.https://www.mdpi.com/2076-393X/10/11/1952COVID-19neutralizing antibodiesspike antibodiesreceiver operating characteristic (ROC) curvecutoff point |
| spellingShingle | Engy Mohamed El-Ghitany Mona H. Hashish Azza Galal Farghaly Eman A. Omran Determining the SARS-CoV-2 Anti-Spike Cutoff Level Denoting Neutralizing Activity Using Two Commercial Kits Vaccines COVID-19 neutralizing antibodies spike antibodies receiver operating characteristic (ROC) curve cutoff point |
| title | Determining the SARS-CoV-2 Anti-Spike Cutoff Level Denoting Neutralizing Activity Using Two Commercial Kits |
| title_full | Determining the SARS-CoV-2 Anti-Spike Cutoff Level Denoting Neutralizing Activity Using Two Commercial Kits |
| title_fullStr | Determining the SARS-CoV-2 Anti-Spike Cutoff Level Denoting Neutralizing Activity Using Two Commercial Kits |
| title_full_unstemmed | Determining the SARS-CoV-2 Anti-Spike Cutoff Level Denoting Neutralizing Activity Using Two Commercial Kits |
| title_short | Determining the SARS-CoV-2 Anti-Spike Cutoff Level Denoting Neutralizing Activity Using Two Commercial Kits |
| title_sort | determining the sars cov 2 anti spike cutoff level denoting neutralizing activity using two commercial kits |
| topic | COVID-19 neutralizing antibodies spike antibodies receiver operating characteristic (ROC) curve cutoff point |
| url | https://www.mdpi.com/2076-393X/10/11/1952 |
| work_keys_str_mv | AT engymohamedelghitany determiningthesarscov2antispikecutoffleveldenotingneutralizingactivityusingtwocommercialkits AT monahhashish determiningthesarscov2antispikecutoffleveldenotingneutralizingactivityusingtwocommercialkits AT azzagalalfarghaly determiningthesarscov2antispikecutoffleveldenotingneutralizingactivityusingtwocommercialkits AT emanaomran determiningthesarscov2antispikecutoffleveldenotingneutralizingactivityusingtwocommercialkits |