Magnetic resonance measurements of cellular and sub-cellular membrane structures in live and fixed neural tissue
We develop magnetic resonance (MR) methods for real-time measurement of tissue microstructure and membrane permeability of live and fixed excised neonatal mouse spinal cords. Diffusion and exchange MR measurements are performed using the strong static gradient produced by a single-sided permanent ma...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2019-12-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/51101 |
| _version_ | 1811201352012922880 |
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| author | Nathan H Williamson Rea Ravin Dan Benjamini Hellmut Merkle Melanie Falgairolle Michael James O'Donovan Dvir Blivis Dave Ide Teddy X Cai Nima S Ghorashi Ruiliang Bai Peter J Basser |
| author_facet | Nathan H Williamson Rea Ravin Dan Benjamini Hellmut Merkle Melanie Falgairolle Michael James O'Donovan Dvir Blivis Dave Ide Teddy X Cai Nima S Ghorashi Ruiliang Bai Peter J Basser |
| author_sort | Nathan H Williamson |
| collection | DOAJ |
| description | We develop magnetic resonance (MR) methods for real-time measurement of tissue microstructure and membrane permeability of live and fixed excised neonatal mouse spinal cords. Diffusion and exchange MR measurements are performed using the strong static gradient produced by a single-sided permanent magnet. Using tissue delipidation methods, we show that water diffusion is restricted solely by lipid membranes. Most of the diffusion signal can be assigned to water in tissue which is far from membranes. The remaining 25% can be assigned to water restricted on length scales of roughly a micron or less, near or within membrane structures at the cellular, organelle, and vesicle levels. Diffusion exchange spectroscopy measures water exchanging between membrane structures and free environments at 100 s-1. |
| first_indexed | 2024-04-12T02:20:25Z |
| format | Article |
| id | doaj.art-c6dd056a9879411ea44d82d52f44469a |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T02:20:25Z |
| publishDate | 2019-12-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-c6dd056a9879411ea44d82d52f44469a2022-12-22T03:52:08ZengeLife Sciences Publications LtdeLife2050-084X2019-12-01810.7554/eLife.51101Magnetic resonance measurements of cellular and sub-cellular membrane structures in live and fixed neural tissueNathan H Williamson0https://orcid.org/0000-0003-0221-9121Rea Ravin1Dan Benjamini2Hellmut Merkle3Melanie Falgairolle4https://orcid.org/0000-0001-5243-4714Michael James O'Donovan5https://orcid.org/0000-0003-2487-7547Dvir Blivis6https://orcid.org/0000-0001-6203-7325Dave Ide7Teddy X Cai8Nima S Ghorashi9Ruiliang Bai10Peter J Basser11https://orcid.org/0000-0003-4795-6088Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, United StatesEunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, United States; Celoptics, Rockville, United StatesEunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, United States; Center for Neuroscience and Regenerative Medicine, Henry Jackson Foundation, Bethesda, United StatesNational Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, United StatesNational Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, United StatesNational Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, United StatesNational Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, United StatesNational Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, United States; National Institute of Mental Health, National Institutes of Health, Bethesda, United StatesEunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, United StatesCardiovascular Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, United StatesEunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, United States; Interdisciplinary Institute of Neuroscience and Technology, School of Medicine, Zhejiang University, Hangzhou, ChinaEunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, United StatesWe develop magnetic resonance (MR) methods for real-time measurement of tissue microstructure and membrane permeability of live and fixed excised neonatal mouse spinal cords. Diffusion and exchange MR measurements are performed using the strong static gradient produced by a single-sided permanent magnet. Using tissue delipidation methods, we show that water diffusion is restricted solely by lipid membranes. Most of the diffusion signal can be assigned to water in tissue which is far from membranes. The remaining 25% can be assigned to water restricted on length scales of roughly a micron or less, near or within membrane structures at the cellular, organelle, and vesicle levels. Diffusion exchange spectroscopy measures water exchanging between membrane structures and free environments at 100 s-1.https://elifesciences.org/articles/51101membrane structurenuclear magnetic resonancewater diffusionmembrane permeabilityspinal cordporous media |
| spellingShingle | Nathan H Williamson Rea Ravin Dan Benjamini Hellmut Merkle Melanie Falgairolle Michael James O'Donovan Dvir Blivis Dave Ide Teddy X Cai Nima S Ghorashi Ruiliang Bai Peter J Basser Magnetic resonance measurements of cellular and sub-cellular membrane structures in live and fixed neural tissue eLife membrane structure nuclear magnetic resonance water diffusion membrane permeability spinal cord porous media |
| title | Magnetic resonance measurements of cellular and sub-cellular membrane structures in live and fixed neural tissue |
| title_full | Magnetic resonance measurements of cellular and sub-cellular membrane structures in live and fixed neural tissue |
| title_fullStr | Magnetic resonance measurements of cellular and sub-cellular membrane structures in live and fixed neural tissue |
| title_full_unstemmed | Magnetic resonance measurements of cellular and sub-cellular membrane structures in live and fixed neural tissue |
| title_short | Magnetic resonance measurements of cellular and sub-cellular membrane structures in live and fixed neural tissue |
| title_sort | magnetic resonance measurements of cellular and sub cellular membrane structures in live and fixed neural tissue |
| topic | membrane structure nuclear magnetic resonance water diffusion membrane permeability spinal cord porous media |
| url | https://elifesciences.org/articles/51101 |
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