Determination of flavonoids by LC/MS and anti-inflammatory activity in Moringa oleifera
Moringa oleifera is an important multipurpose tropical tree under-recognized for its nutritional and medicinal properties. Leaves of moringa collected from sub-Sahara Africa were analyzed for phenolic components by HPLC–UV–MS. Twelve flavonoids were identified, including quercetin and kaempferol glu...
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| Format: | Article |
| Language: | English |
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Elsevier
2013-10-01
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| Series: | Journal of Functional Foods |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464613002090 |
| _version_ | 1818406158210170880 |
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| author | Julia P. Coppin Yanping Xu Hong Chen Min-Hsiung Pan Chi-Tang Ho Rodolfo Juliani James E. Simon Qingli Wu |
| author_facet | Julia P. Coppin Yanping Xu Hong Chen Min-Hsiung Pan Chi-Tang Ho Rodolfo Juliani James E. Simon Qingli Wu |
| author_sort | Julia P. Coppin |
| collection | DOAJ |
| description | Moringa oleifera is an important multipurpose tropical tree under-recognized for its nutritional and medicinal properties. Leaves of moringa collected from sub-Sahara Africa were analyzed for phenolic components by HPLC–UV–MS. Twelve flavonoids were identified, including quercetin and kaempferol glucosides and glucoside malonates as major constituents. To facilitate quantitative analysis, acid hydrolysis during extraction of moringa samples was employed to convert the conjugates into their respective flavonoid aglycones, allowing accurate quantitation of total flavonoids as aglycones. Validation of the method demonstrated recoveries ranging from 92.6% to 107.5% for the two analytes. Using this method, total flavonoids from moringa collected from Ghana, Senegal and Zambia were determined and their content ranged from 0.18% to 1.64% (g/dry weight) across the respective environment or country of production and by variety. Moringa was found to exhibit anti-inflammatory activity of constituents-rich varieties. As flavonoid malonates are among the major constituents in the original plant material, a study examining thermal stability of flavonoid malonyl derivatives showed that these compounds were not thermally stable and easily cleaved to yield their respective flavonoid glycosides. |
| first_indexed | 2024-12-14T09:07:29Z |
| format | Article |
| id | doaj.art-c6ddded463b04bdeac2e66b214635993 |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-12-14T09:07:29Z |
| publishDate | 2013-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-c6ddded463b04bdeac2e66b2146359932022-12-21T23:08:40ZengElsevierJournal of Functional Foods1756-46462013-10-015418921899Determination of flavonoids by LC/MS and anti-inflammatory activity in Moringa oleiferaJulia P. Coppin0Yanping Xu1Hong Chen2Min-Hsiung Pan3Chi-Tang Ho4Rodolfo Juliani5James E. Simon6Qingli Wu7New Use Agriculture and Natural Plant Products Program, Department of Plant Biology and Pathology, Rutgers University, 59 Dudley Road, New Brunswick, NJ 08901, USANew Use Agriculture and Natural Plant Products Program, Department of Plant Biology and Pathology, Rutgers University, 59 Dudley Road, New Brunswick, NJ 08901, USANew Use Agriculture and Natural Plant Products Program, Department of Plant Biology and Pathology, Rutgers University, 59 Dudley Road, New Brunswick, NJ 08901, USADepartment of Seafood Science, National Kaohsiung Marine University, Kaohsiung 811, TaiwanDepartment of Food Science, Rutgers University, 65 Dudley Road, New Brunswick, NJ 08901, USANew Use Agriculture and Natural Plant Products Program, Department of Plant Biology and Pathology, Rutgers University, 59 Dudley Road, New Brunswick, NJ 08901, USANew Use Agriculture and Natural Plant Products Program, Department of Plant Biology and Pathology, Rutgers University, 59 Dudley Road, New Brunswick, NJ 08901, USANew Use Agriculture and Natural Plant Products Program, Department of Plant Biology and Pathology, Rutgers University, 59 Dudley Road, New Brunswick, NJ 08901, USA; Corresponding author. Tel.: +1 848 932 6238; fax: +1 732 932 9441.Moringa oleifera is an important multipurpose tropical tree under-recognized for its nutritional and medicinal properties. Leaves of moringa collected from sub-Sahara Africa were analyzed for phenolic components by HPLC–UV–MS. Twelve flavonoids were identified, including quercetin and kaempferol glucosides and glucoside malonates as major constituents. To facilitate quantitative analysis, acid hydrolysis during extraction of moringa samples was employed to convert the conjugates into their respective flavonoid aglycones, allowing accurate quantitation of total flavonoids as aglycones. Validation of the method demonstrated recoveries ranging from 92.6% to 107.5% for the two analytes. Using this method, total flavonoids from moringa collected from Ghana, Senegal and Zambia were determined and their content ranged from 0.18% to 1.64% (g/dry weight) across the respective environment or country of production and by variety. Moringa was found to exhibit anti-inflammatory activity of constituents-rich varieties. As flavonoid malonates are among the major constituents in the original plant material, a study examining thermal stability of flavonoid malonyl derivatives showed that these compounds were not thermally stable and easily cleaved to yield their respective flavonoid glycosides.http://www.sciencedirect.com/science/article/pii/S1756464613002090Moringa oleiferaLC/UV/MSDFlavonoidAnti-inflammationAcid hydrolysisThermal stability |
| spellingShingle | Julia P. Coppin Yanping Xu Hong Chen Min-Hsiung Pan Chi-Tang Ho Rodolfo Juliani James E. Simon Qingli Wu Determination of flavonoids by LC/MS and anti-inflammatory activity in Moringa oleifera Journal of Functional Foods Moringa oleifera LC/UV/MSD Flavonoid Anti-inflammation Acid hydrolysis Thermal stability |
| title | Determination of flavonoids by LC/MS and anti-inflammatory activity in Moringa oleifera |
| title_full | Determination of flavonoids by LC/MS and anti-inflammatory activity in Moringa oleifera |
| title_fullStr | Determination of flavonoids by LC/MS and anti-inflammatory activity in Moringa oleifera |
| title_full_unstemmed | Determination of flavonoids by LC/MS and anti-inflammatory activity in Moringa oleifera |
| title_short | Determination of flavonoids by LC/MS and anti-inflammatory activity in Moringa oleifera |
| title_sort | determination of flavonoids by lc ms and anti inflammatory activity in moringa oleifera |
| topic | Moringa oleifera LC/UV/MSD Flavonoid Anti-inflammation Acid hydrolysis Thermal stability |
| url | http://www.sciencedirect.com/science/article/pii/S1756464613002090 |
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