Lack of Association of C677T Methylenetetrahydrofolate Reductase Polymorphism with Breast Cancer Risk in Mali
Methylenetetrahydrofolate reductase (MTHFR) plays a major role in the metabolism of folates and homocysteine, which in turn can affect gene expression and ultimately promote the development of breast cancer. Thus, mutations in the MTHFR gene could influence homocysteine, methionine, and S-adenosylme...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Hindawi - Cambridge University Press
2023-01-01
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| Series: | Genetics Research |
| Online Access: | http://dx.doi.org/10.1155/2023/4683831 |
| _version_ | 1811175581456269312 |
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| author | Brehima Diakite Yaya Kassogue Mamoudou Maiga Guimogo Dolo Oumar Kassogue Jane L. Holl Brian Joyce Jun Wang Kadidiatou Cisse Fousseyni Diarra Mamadou L. Keita Cheick B. Traore Bakarou Kamate Sidi B. Sissoko Bourama Coulibaly Adama S. Sissoko Drissa Traore Fatoumata M. Sidibe Sekou Bah Ibrahim Teguete Madani Ly Sellama Nadifi Hind Dehbi Kyeezu Kim Robert Murphy Lifang Hou |
| author_facet | Brehima Diakite Yaya Kassogue Mamoudou Maiga Guimogo Dolo Oumar Kassogue Jane L. Holl Brian Joyce Jun Wang Kadidiatou Cisse Fousseyni Diarra Mamadou L. Keita Cheick B. Traore Bakarou Kamate Sidi B. Sissoko Bourama Coulibaly Adama S. Sissoko Drissa Traore Fatoumata M. Sidibe Sekou Bah Ibrahim Teguete Madani Ly Sellama Nadifi Hind Dehbi Kyeezu Kim Robert Murphy Lifang Hou |
| author_sort | Brehima Diakite |
| collection | DOAJ |
| description | Methylenetetrahydrofolate reductase (MTHFR) plays a major role in the metabolism of folates and homocysteine, which in turn can affect gene expression and ultimately promote the development of breast cancer. Thus, mutations in the MTHFR gene could influence homocysteine, methionine, and S-adenosylmethionine levels and, indirectly, nucleotide levels. Imbalance in methionine and S-adenosylmethionine synthesis affects protein synthesis and methylation. These changes, which affect gene expression, may ultimately promote the development of breast cancer. We therefore hypothesized that such mutations could also play an important role in the occurrence and pathogenesis of breast cancer in a Malian population. In this study, we used the PCR-RFLP technique to identify the different genotypic profiles of the C677T MTHFR polymorphism in 127 breast cancer women and 160 healthy controls. The genotypic distribution of the C677T polymorphism in breast cancer cases was 88.2% for CC, 11.0% for CT, and 0.8% for TT. Healthy controls showed a similar distribution with 90.6% for CC, 8.8% for CT, and 0.6% for TT. We found no statistical association between the C677T polymorphism and breast cancer risk for the codominant models CT and TT p>0.05. The same trend was observed when the analysis was extended to other genetic models, including dominant (p = 0.50), recessive (p = 0.87), and additive (p = 0.50) models. The C677T polymorphism of MTHFR gene did not influence the risk of breast cancer in the Malian samples. |
| first_indexed | 2024-04-10T19:39:10Z |
| format | Article |
| id | doaj.art-c6e4b6a63bd044518455cbf06f904673 |
| institution | Directory Open Access Journal |
| issn | 1469-5073 |
| language | English |
| last_indexed | 2024-04-10T19:39:10Z |
| publishDate | 2023-01-01 |
| publisher | Hindawi - Cambridge University Press |
| record_format | Article |
| series | Genetics Research |
| spelling | doaj.art-c6e4b6a63bd044518455cbf06f9046732023-01-30T00:11:14ZengHindawi - Cambridge University PressGenetics Research1469-50732023-01-01202310.1155/2023/4683831Lack of Association of C677T Methylenetetrahydrofolate Reductase Polymorphism with Breast Cancer Risk in MaliBrehima Diakite0Yaya Kassogue1Mamoudou Maiga2Guimogo Dolo3Oumar Kassogue4Jane L. Holl5Brian Joyce6Jun Wang7Kadidiatou Cisse8Fousseyni Diarra9Mamadou L. Keita10Cheick B. Traore11Bakarou Kamate12Sidi B. Sissoko13Bourama Coulibaly14Adama S. Sissoko15Drissa Traore16Fatoumata M. Sidibe17Sekou Bah18Ibrahim Teguete19Madani Ly20Sellama Nadifi21Hind Dehbi22Kyeezu Kim23Robert Murphy24Lifang Hou25Faculty of Medicine and OdontostomatologyFaculty of Medicine and OdontostomatologyFaculty of Medicine and OdontostomatologyFaculty of Medicine and OdontostomatologyFaculty of Medicine and OdontostomatologyDepartment of NeurologyInstitute for Global HealthPreventive Medicine DepartmentFaculty of Medicine and OdontostomatologyFaculty of Medicine and OdontostomatologyFaculty of Medicine and OdontostomatologyFaculty of Medicine and OdontostomatologyFaculty of Medicine and OdontostomatologyFaculty of Medicine and OdontostomatologyFaculty of Medicine and OdontostomatologyFaculty of Medicine and OdontostomatologyFaculty of Medicine and OdontostomatologyFaculty of Medicine and OdontostomatologyFaculty of PharmacyFaculty of Medicine and OdontostomatologyFaculty of Medicine and OdontostomatologyHassan II University Aïn ChockHassan II University Aïn ChockInstitute for Global HealthInstitute for Global HealthInstitute for Global HealthMethylenetetrahydrofolate reductase (MTHFR) plays a major role in the metabolism of folates and homocysteine, which in turn can affect gene expression and ultimately promote the development of breast cancer. Thus, mutations in the MTHFR gene could influence homocysteine, methionine, and S-adenosylmethionine levels and, indirectly, nucleotide levels. Imbalance in methionine and S-adenosylmethionine synthesis affects protein synthesis and methylation. These changes, which affect gene expression, may ultimately promote the development of breast cancer. We therefore hypothesized that such mutations could also play an important role in the occurrence and pathogenesis of breast cancer in a Malian population. In this study, we used the PCR-RFLP technique to identify the different genotypic profiles of the C677T MTHFR polymorphism in 127 breast cancer women and 160 healthy controls. The genotypic distribution of the C677T polymorphism in breast cancer cases was 88.2% for CC, 11.0% for CT, and 0.8% for TT. Healthy controls showed a similar distribution with 90.6% for CC, 8.8% for CT, and 0.6% for TT. We found no statistical association between the C677T polymorphism and breast cancer risk for the codominant models CT and TT p>0.05. The same trend was observed when the analysis was extended to other genetic models, including dominant (p = 0.50), recessive (p = 0.87), and additive (p = 0.50) models. The C677T polymorphism of MTHFR gene did not influence the risk of breast cancer in the Malian samples.http://dx.doi.org/10.1155/2023/4683831 |
| spellingShingle | Brehima Diakite Yaya Kassogue Mamoudou Maiga Guimogo Dolo Oumar Kassogue Jane L. Holl Brian Joyce Jun Wang Kadidiatou Cisse Fousseyni Diarra Mamadou L. Keita Cheick B. Traore Bakarou Kamate Sidi B. Sissoko Bourama Coulibaly Adama S. Sissoko Drissa Traore Fatoumata M. Sidibe Sekou Bah Ibrahim Teguete Madani Ly Sellama Nadifi Hind Dehbi Kyeezu Kim Robert Murphy Lifang Hou Lack of Association of C677T Methylenetetrahydrofolate Reductase Polymorphism with Breast Cancer Risk in Mali Genetics Research |
| title | Lack of Association of C677T Methylenetetrahydrofolate Reductase Polymorphism with Breast Cancer Risk in Mali |
| title_full | Lack of Association of C677T Methylenetetrahydrofolate Reductase Polymorphism with Breast Cancer Risk in Mali |
| title_fullStr | Lack of Association of C677T Methylenetetrahydrofolate Reductase Polymorphism with Breast Cancer Risk in Mali |
| title_full_unstemmed | Lack of Association of C677T Methylenetetrahydrofolate Reductase Polymorphism with Breast Cancer Risk in Mali |
| title_short | Lack of Association of C677T Methylenetetrahydrofolate Reductase Polymorphism with Breast Cancer Risk in Mali |
| title_sort | lack of association of c677t methylenetetrahydrofolate reductase polymorphism with breast cancer risk in mali |
| url | http://dx.doi.org/10.1155/2023/4683831 |
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