Evaluation of SCD, ACACA and FASN Mutations: Effects on Pork Quality and Other Production Traits in Pigs Selected Based on RNA-Seq Results
In recent years, pig producers have struggled with the problem of low intramuscular fat levels in pork, which impacts palatability and ultimately meat quality. Reduced levels of intramuscular fat are likely the result of breeding objectives aimed at increasing lean meat content. In this study, three...
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MDPI AG
2020-01-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/10/1/123 |
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| author | Katarzyna Piórkowska Martyna Małopolska Katarzyna Ropka-Molik Magdalena Szyndler-Nędza Angelika Wiechniak Kacper Żukowski Barry Lambert Mirosław Tyra |
| author_facet | Katarzyna Piórkowska Martyna Małopolska Katarzyna Ropka-Molik Magdalena Szyndler-Nędza Angelika Wiechniak Kacper Żukowski Barry Lambert Mirosław Tyra |
| author_sort | Katarzyna Piórkowska |
| collection | DOAJ |
| description | In recent years, pig producers have struggled with the problem of low intramuscular fat levels in pork, which impacts palatability and ultimately meat quality. Reduced levels of intramuscular fat are likely the result of breeding objectives aimed at increasing lean meat content. In this study, three mutations within candidate genes for fat content (<i>SCD, ACACA,</i> and <i>FASN)</i> were selected, based on RNA-seq results and the relationship between polymorphisms in genes related to lipid metabolism, fattening and slaughter characteristics, as well as pork quality, including IMF level, were evaluated to identify selection markers. Moreover, their impact on gene expression was also examined. The PCR−RFLP (polymerase cha- in reaction − restriction fragments length) method was used to establish genotypes and effect sizes of potential genetic markers were estimated using a GLM model. It was identified that a <i>FASN</i> missense variant was positively associated with the expression level of this gene, which suggested its linkage with a mutation having a regulatory function. The association study indicated that the FASN missense variant may play a role in the determination of feed conversion and meat colour. In turn, a mutation in the <i>ACACA</i> gene showed a relationship with IMF content in the Puławska breed where the differences reached as much as 20%. We suggest considering all three mutations in further studies based on different pig populations due to the crucial role of <i>SCD, ACACA,</i> and <i>FASN</i> genes in lipid metabolism. |
| first_indexed | 2024-12-19T10:15:20Z |
| format | Article |
| id | doaj.art-c6e6f1dc679f40bcae519aa1aa1b0508 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-12-19T10:15:20Z |
| publishDate | 2020-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-c6e6f1dc679f40bcae519aa1aa1b05082022-12-21T20:26:15ZengMDPI AGAnimals2076-26152020-01-0110112310.3390/ani10010123ani10010123Evaluation of SCD, ACACA and FASN Mutations: Effects on Pork Quality and Other Production Traits in Pigs Selected Based on RNA-Seq ResultsKatarzyna Piórkowska0Martyna Małopolska1Katarzyna Ropka-Molik2Magdalena Szyndler-Nędza3Angelika Wiechniak4Kacper Żukowski5Barry Lambert6Mirosław Tyra7Department of Animal Molecular Biology, National Research Institute of Animal Production, Krakowska 1, 32-083 Balice, PolandDepartment of Pig Breeding, National Research Institute of Animal Production, Krakowska 1, 32-083 Balice, PolandDepartment of Animal Molecular Biology, National Research Institute of Animal Production, Krakowska 1, 32-083 Balice, PolandDepartment of Pig Breeding, National Research Institute of Animal Production, Krakowska 1, 32-083 Balice, PolandFaculty of Biotechnology and Horticulture, University of Agriculture in Kraków, al. 29 Listopada 54, 31-425 Kraków, PolandDepartment of Cattle Breeding, National Research Institute of Animal Production, Krakowska 1, 32-083 Balice, PolandDepartment of Animal Science and Veterinary Technology, Tarleton State University, Stephenville, TX 76402, USADepartment of Pig Breeding, National Research Institute of Animal Production, Krakowska 1, 32-083 Balice, PolandIn recent years, pig producers have struggled with the problem of low intramuscular fat levels in pork, which impacts palatability and ultimately meat quality. Reduced levels of intramuscular fat are likely the result of breeding objectives aimed at increasing lean meat content. In this study, three mutations within candidate genes for fat content (<i>SCD, ACACA,</i> and <i>FASN)</i> were selected, based on RNA-seq results and the relationship between polymorphisms in genes related to lipid metabolism, fattening and slaughter characteristics, as well as pork quality, including IMF level, were evaluated to identify selection markers. Moreover, their impact on gene expression was also examined. The PCR−RFLP (polymerase cha- in reaction − restriction fragments length) method was used to establish genotypes and effect sizes of potential genetic markers were estimated using a GLM model. It was identified that a <i>FASN</i> missense variant was positively associated with the expression level of this gene, which suggested its linkage with a mutation having a regulatory function. The association study indicated that the FASN missense variant may play a role in the determination of feed conversion and meat colour. In turn, a mutation in the <i>ACACA</i> gene showed a relationship with IMF content in the Puławska breed where the differences reached as much as 20%. We suggest considering all three mutations in further studies based on different pig populations due to the crucial role of <i>SCD, ACACA,</i> and <i>FASN</i> genes in lipid metabolism.https://www.mdpi.com/2076-2615/10/1/123rna-seqimfselection markerpigmeat quality |
| spellingShingle | Katarzyna Piórkowska Martyna Małopolska Katarzyna Ropka-Molik Magdalena Szyndler-Nędza Angelika Wiechniak Kacper Żukowski Barry Lambert Mirosław Tyra Evaluation of SCD, ACACA and FASN Mutations: Effects on Pork Quality and Other Production Traits in Pigs Selected Based on RNA-Seq Results Animals rna-seq imf selection marker pig meat quality |
| title | Evaluation of SCD, ACACA and FASN Mutations: Effects on Pork Quality and Other Production Traits in Pigs Selected Based on RNA-Seq Results |
| title_full | Evaluation of SCD, ACACA and FASN Mutations: Effects on Pork Quality and Other Production Traits in Pigs Selected Based on RNA-Seq Results |
| title_fullStr | Evaluation of SCD, ACACA and FASN Mutations: Effects on Pork Quality and Other Production Traits in Pigs Selected Based on RNA-Seq Results |
| title_full_unstemmed | Evaluation of SCD, ACACA and FASN Mutations: Effects on Pork Quality and Other Production Traits in Pigs Selected Based on RNA-Seq Results |
| title_short | Evaluation of SCD, ACACA and FASN Mutations: Effects on Pork Quality and Other Production Traits in Pigs Selected Based on RNA-Seq Results |
| title_sort | evaluation of scd acaca and fasn mutations effects on pork quality and other production traits in pigs selected based on rna seq results |
| topic | rna-seq imf selection marker pig meat quality |
| url | https://www.mdpi.com/2076-2615/10/1/123 |
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