CRISPR-Based Genome Editing: Advancements and Opportunities for Rice Improvement
To increase the potentiality of crop production for future food security, new technologies for plant breeding are required, including genome editing technology—being one of the most promising. Genome editing with the CRISPR/Cas system has attracted researchers in the last decade as a safer and easie...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-04-01
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| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/23/8/4454 |
| _version_ | 1797434520797970432 |
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| author | Workie Anley Zegeye Mesfin Tsegaw Yingxin Zhang Liyong Cao |
| author_facet | Workie Anley Zegeye Mesfin Tsegaw Yingxin Zhang Liyong Cao |
| author_sort | Workie Anley Zegeye |
| collection | DOAJ |
| description | To increase the potentiality of crop production for future food security, new technologies for plant breeding are required, including genome editing technology—being one of the most promising. Genome editing with the CRISPR/Cas system has attracted researchers in the last decade as a safer and easier tool for genome editing in a variety of living organisms including rice. Genome editing has transformed agriculture by reducing biotic and abiotic stresses and increasing yield. Recently, genome editing technologies have been developed quickly in order to avoid the challenges that genetically modified crops face. Developing transgenic-free edited plants without introducing foreign DNA has received regulatory approval in a number of countries. Several ongoing efforts from various countries are rapidly expanding to adopt the innovations. This review covers the mechanisms of CRISPR/Cas9, comparisons of CRISPR/Cas9 with other gene-editing technologies—including newly emerged Cas variants—and focuses on CRISPR/Cas9-targeted genes for rice crop improvement. We have further highlighted CRISPR/Cas9 vector construction model design and different bioinformatics tools for target site selection. |
| first_indexed | 2024-03-09T10:34:30Z |
| format | Article |
| id | doaj.art-c6ea4123bc924f439bc026a0ba4666f9 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T10:34:30Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-c6ea4123bc924f439bc026a0ba4666f92023-12-01T21:04:59ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-04-01238445410.3390/ijms23084454CRISPR-Based Genome Editing: Advancements and Opportunities for Rice ImprovementWorkie Anley Zegeye0Mesfin Tsegaw1Yingxin Zhang2Liyong Cao3State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, ChinaDepartment of Agricultural Biotechnology, Institute of Biotechnology, University of Gondar, Gondar P.O. Box 196, EthiopiaState Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, ChinaState Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, ChinaTo increase the potentiality of crop production for future food security, new technologies for plant breeding are required, including genome editing technology—being one of the most promising. Genome editing with the CRISPR/Cas system has attracted researchers in the last decade as a safer and easier tool for genome editing in a variety of living organisms including rice. Genome editing has transformed agriculture by reducing biotic and abiotic stresses and increasing yield. Recently, genome editing technologies have been developed quickly in order to avoid the challenges that genetically modified crops face. Developing transgenic-free edited plants without introducing foreign DNA has received regulatory approval in a number of countries. Several ongoing efforts from various countries are rapidly expanding to adopt the innovations. This review covers the mechanisms of CRISPR/Cas9, comparisons of CRISPR/Cas9 with other gene-editing technologies—including newly emerged Cas variants—and focuses on CRISPR/Cas9-targeted genes for rice crop improvement. We have further highlighted CRISPR/Cas9 vector construction model design and different bioinformatics tools for target site selection.https://www.mdpi.com/1422-0067/23/8/4454CRISPR/Cas9Cas variantsgenome editingriceimprovements |
| spellingShingle | Workie Anley Zegeye Mesfin Tsegaw Yingxin Zhang Liyong Cao CRISPR-Based Genome Editing: Advancements and Opportunities for Rice Improvement International Journal of Molecular Sciences CRISPR/Cas9 Cas variants genome editing rice improvements |
| title | CRISPR-Based Genome Editing: Advancements and Opportunities for Rice Improvement |
| title_full | CRISPR-Based Genome Editing: Advancements and Opportunities for Rice Improvement |
| title_fullStr | CRISPR-Based Genome Editing: Advancements and Opportunities for Rice Improvement |
| title_full_unstemmed | CRISPR-Based Genome Editing: Advancements and Opportunities for Rice Improvement |
| title_short | CRISPR-Based Genome Editing: Advancements and Opportunities for Rice Improvement |
| title_sort | crispr based genome editing advancements and opportunities for rice improvement |
| topic | CRISPR/Cas9 Cas variants genome editing rice improvements |
| url | https://www.mdpi.com/1422-0067/23/8/4454 |
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