Prebiotic Activity of Polysaccharides Extracted from Gigantochloa Levis (Buluh beting) Shoots

Bamboo shoot crude polysaccharides (BSCP) extracted from the shoots of Gigantochloa levis gave about 3.27 ± 0.18% on dry basis and a very minute percentage of protein (0.02 ± 0.01%). The molecular weight of BSCP estimated by gel chromatography was found to be around 7.49 × 103 Da, while the molecula...

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Main Authors: Aida Firdaus Muhammad Nurul Azmi, Yazid Abdul Manap, Shuhaimi Mustafa, Dzulkifly Md. Hashim
Format: Article
Language:English
Published: MDPI AG 2012-02-01
Series:Molecules
Subjects:
Online Access:http://www.mdpi.com/1420-3049/17/2/1635/
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author Aida Firdaus Muhammad Nurul Azmi
Yazid Abdul Manap
Shuhaimi Mustafa
Dzulkifly Md. Hashim
author_facet Aida Firdaus Muhammad Nurul Azmi
Yazid Abdul Manap
Shuhaimi Mustafa
Dzulkifly Md. Hashim
author_sort Aida Firdaus Muhammad Nurul Azmi
collection DOAJ
description Bamboo shoot crude polysaccharides (BSCP) extracted from the shoots of Gigantochloa levis gave about 3.27 ± 0.18% on dry basis and a very minute percentage of protein (0.02 ± 0.01%). The molecular weight of BSCP estimated by gel chromatography was found to be around 7.49 × 103 Da, while the molecular weights of purified fractions (F1 to F5) were around 1550.96, 1471.63, 1685.78, 1691.61 and 1551.67 Da, respectively. The FTIR spectrum of BSCP revealed the possibility that the extract contains β-glucan, which can be considered a valuable compound for the medical and food industries. These relate to the resistance of BSCP towards artificial human gastric juice which is more than 99%. Prebiotic activity tested using BSCP as a carbon source showed significant increase in the growth of B. animalis ATCC 1053, B. longum BB 536 and L. acidophilus ATCC 4356 as compared to the use of FOS. Survivality of S. choleraesuis JCM 6977 was found to be slower in both BSCP and FOS. Study conducted reflects a good sign for the BSCP to be exploited as a promising prebiotic.
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spelling doaj.art-c6ee57accc1f4b03b8c59aae2acffa292022-12-22T01:37:41ZengMDPI AGMolecules1420-30492012-02-011721635165110.3390/molecules17021635Prebiotic Activity of Polysaccharides Extracted from Gigantochloa Levis (Buluh beting) ShootsAida Firdaus Muhammad Nurul AzmiYazid Abdul ManapShuhaimi MustafaDzulkifly Md. HashimBamboo shoot crude polysaccharides (BSCP) extracted from the shoots of Gigantochloa levis gave about 3.27 ± 0.18% on dry basis and a very minute percentage of protein (0.02 ± 0.01%). The molecular weight of BSCP estimated by gel chromatography was found to be around 7.49 × 103 Da, while the molecular weights of purified fractions (F1 to F5) were around 1550.96, 1471.63, 1685.78, 1691.61 and 1551.67 Da, respectively. The FTIR spectrum of BSCP revealed the possibility that the extract contains β-glucan, which can be considered a valuable compound for the medical and food industries. These relate to the resistance of BSCP towards artificial human gastric juice which is more than 99%. Prebiotic activity tested using BSCP as a carbon source showed significant increase in the growth of B. animalis ATCC 1053, B. longum BB 536 and L. acidophilus ATCC 4356 as compared to the use of FOS. Survivality of S. choleraesuis JCM 6977 was found to be slower in both BSCP and FOS. Study conducted reflects a good sign for the BSCP to be exploited as a promising prebiotic.http://www.mdpi.com/1420-3049/17/2/1635/polysaccharidesFOSprebioticsprobioticsoptical density
spellingShingle Aida Firdaus Muhammad Nurul Azmi
Yazid Abdul Manap
Shuhaimi Mustafa
Dzulkifly Md. Hashim
Prebiotic Activity of Polysaccharides Extracted from Gigantochloa Levis (Buluh beting) Shoots
Molecules
polysaccharides
FOS
prebiotics
probiotics
optical density
title Prebiotic Activity of Polysaccharides Extracted from Gigantochloa Levis (Buluh beting) Shoots
title_full Prebiotic Activity of Polysaccharides Extracted from Gigantochloa Levis (Buluh beting) Shoots
title_fullStr Prebiotic Activity of Polysaccharides Extracted from Gigantochloa Levis (Buluh beting) Shoots
title_full_unstemmed Prebiotic Activity of Polysaccharides Extracted from Gigantochloa Levis (Buluh beting) Shoots
title_short Prebiotic Activity of Polysaccharides Extracted from Gigantochloa Levis (Buluh beting) Shoots
title_sort prebiotic activity of polysaccharides extracted from gigantochloa levis buluh beting shoots
topic polysaccharides
FOS
prebiotics
probiotics
optical density
url http://www.mdpi.com/1420-3049/17/2/1635/
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