Traversing the cell: Agrobacterium T-DNA’s journey to the host genome
The genus Agrobacterium is unique in its ability to conduct interkingdom genetic exchange. Virulent Agrobacterium strains transfer single-strand forms of T-DNA (T-strands) and several Virulence effector proteins through a bacterial type IV secretion system into plant host cells. T-strands must trav...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2012-03-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fpls.2012.00052/full |
| _version_ | 1811334830748598272 |
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| author | Stanton B. Gelvin |
| author_facet | Stanton B. Gelvin |
| author_sort | Stanton B. Gelvin |
| collection | DOAJ |
| description | The genus Agrobacterium is unique in its ability to conduct interkingdom genetic exchange. Virulent Agrobacterium strains transfer single-strand forms of T-DNA (T-strands) and several Virulence effector proteins through a bacterial type IV secretion system into plant host cells. T-strands must traverse the plant wall and plasma membrane, traffic through the cytoplasm, enter the nucleus, and ultimately target host chromatin for stable integration. Because any DNA sequence placed between T-DNA borders can be transferred to plants and integrated into the plant genome, the transfer and intracellular trafficking processes must be mediated by bacterial and host proteins that form complexes with T-strands. This review summarizes current knowledge of proteins that interact with T-strands in the plant cell, and discusses several models of T-complex (T-strand and associated proteins) trafficking. A detailed understanding of how these macromolecular complexes enter the host cell and traverse the plant cytoplasm will require development of novel technologies to follow molecules from their bacterial site of synthesis into the plant cell, and how these transferred molecules interact with host proteins, and cytoplasmic and nuclear structures. |
| first_indexed | 2024-04-13T17:14:59Z |
| format | Article |
| id | doaj.art-c6f142adb2d248f0b7cf9ad2b9ffa5ab |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-13T17:14:59Z |
| publishDate | 2012-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-c6f142adb2d248f0b7cf9ad2b9ffa5ab2022-12-22T02:38:09ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2012-03-01310.3389/fpls.2012.0005221986Traversing the cell: Agrobacterium T-DNA’s journey to the host genomeStanton B. Gelvin0Purdue UniversityThe genus Agrobacterium is unique in its ability to conduct interkingdom genetic exchange. Virulent Agrobacterium strains transfer single-strand forms of T-DNA (T-strands) and several Virulence effector proteins through a bacterial type IV secretion system into plant host cells. T-strands must traverse the plant wall and plasma membrane, traffic through the cytoplasm, enter the nucleus, and ultimately target host chromatin for stable integration. Because any DNA sequence placed between T-DNA borders can be transferred to plants and integrated into the plant genome, the transfer and intracellular trafficking processes must be mediated by bacterial and host proteins that form complexes with T-strands. This review summarizes current knowledge of proteins that interact with T-strands in the plant cell, and discusses several models of T-complex (T-strand and associated proteins) trafficking. A detailed understanding of how these macromolecular complexes enter the host cell and traverse the plant cytoplasm will require development of novel technologies to follow molecules from their bacterial site of synthesis into the plant cell, and how these transferred molecules interact with host proteins, and cytoplasmic and nuclear structures.http://journal.frontiersin.org/Journal/10.3389/fpls.2012.00052/fullAgrobacteriumT-DNAcytoplasmic traffickingnuclear targetingVirulence effector proteins |
| spellingShingle | Stanton B. Gelvin Traversing the cell: Agrobacterium T-DNA’s journey to the host genome Frontiers in Plant Science Agrobacterium T-DNA cytoplasmic trafficking nuclear targeting Virulence effector proteins |
| title | Traversing the cell: Agrobacterium T-DNA’s journey to the host genome |
| title_full | Traversing the cell: Agrobacterium T-DNA’s journey to the host genome |
| title_fullStr | Traversing the cell: Agrobacterium T-DNA’s journey to the host genome |
| title_full_unstemmed | Traversing the cell: Agrobacterium T-DNA’s journey to the host genome |
| title_short | Traversing the cell: Agrobacterium T-DNA’s journey to the host genome |
| title_sort | traversing the cell agrobacterium t dna s journey to the host genome |
| topic | Agrobacterium T-DNA cytoplasmic trafficking nuclear targeting Virulence effector proteins |
| url | http://journal.frontiersin.org/Journal/10.3389/fpls.2012.00052/full |
| work_keys_str_mv | AT stantonbgelvin traversingthecellagrobacteriumtdnasjourneytothehostgenome |