BIIDXI, a DUF642 Cell Wall Protein That Regulates Pectin Methyl Esterase Activity, Is Involved in Thermotolerance Processes in <i>Arabidopsis thaliana</i>
Plant cell wall remodeling is an important process during plant responses to heat stress. Pectins, a group of cell wall polysaccharides with a great diversity of complex chemical structures, are also involved in heat stress responses. Enzymatic activity of the pectin methyl esterases, which remove m...
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| Format: | Article |
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MDPI AG
2022-11-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/11/22/3049 |
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| author | Eduardo Pineda-Hernández José Erik Cruz-Valderrama Ximena Gómez-Maqueo Eleazar Martínez-Barajas Alicia Gamboa-deBuen |
| author_facet | Eduardo Pineda-Hernández José Erik Cruz-Valderrama Ximena Gómez-Maqueo Eleazar Martínez-Barajas Alicia Gamboa-deBuen |
| author_sort | Eduardo Pineda-Hernández |
| collection | DOAJ |
| description | Plant cell wall remodeling is an important process during plant responses to heat stress. Pectins, a group of cell wall polysaccharides with a great diversity of complex chemical structures, are also involved in heat stress responses. Enzymatic activity of the pectin methyl esterases, which remove methyl groups from pectins in the cell wall, is regulated by DUF642 proteins, as described in different plants, including <i>Arabidopsis thaliana</i> and <i>Oryza sativa</i>. Our results demonstrated that heat stress altered the expression of the DUF642 gene, <i>BIIDXI</i>. There was an important decrease in <i>BIIDXI</i> expression during the first hour of HS, followed by an increase at 24 h. <i>bdx</i>-1 seedlings had less tolerance to heat stress but presented a normal heat stress response; <i>HSFA2</i> and <i>HSP22</i> expressions were highly increased, as they were in WT seedlings. Thermopriming triggered changes in pectin methyl esterase activity in WT seedlings, while no increases in PME activity were detected in <i>bdx</i>-1 seedlings at the same conditions. Taken together, our results suggest that BIIDXI is involved in thermotolerance via PME activation. |
| first_indexed | 2024-03-09T18:04:18Z |
| format | Article |
| id | doaj.art-c6f2602538a54b01a433eb20cccfdf54 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-09T18:04:18Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-c6f2602538a54b01a433eb20cccfdf542023-11-24T09:38:48ZengMDPI AGPlants2223-77472022-11-011122304910.3390/plants11223049BIIDXI, a DUF642 Cell Wall Protein That Regulates Pectin Methyl Esterase Activity, Is Involved in Thermotolerance Processes in <i>Arabidopsis thaliana</i>Eduardo Pineda-Hernández0José Erik Cruz-Valderrama1Ximena Gómez-Maqueo2Eleazar Martínez-Barajas3Alicia Gamboa-deBuen4Instituto de Ecología, Universidad Nacional Autónoma de México (UNAM), Ciudad de México 04510, MexicoInstituto de Ecología, Universidad Nacional Autónoma de México (UNAM), Ciudad de México 04510, MexicoInstituto de Ecología, Universidad Nacional Autónoma de México (UNAM), Ciudad de México 04510, MexicoFacultad de Química, Universidad Nacional Autónoma de México (UNAM), Ciudad de México 04510, MexicoInstituto de Ecología, Universidad Nacional Autónoma de México (UNAM), Ciudad de México 04510, MexicoPlant cell wall remodeling is an important process during plant responses to heat stress. Pectins, a group of cell wall polysaccharides with a great diversity of complex chemical structures, are also involved in heat stress responses. Enzymatic activity of the pectin methyl esterases, which remove methyl groups from pectins in the cell wall, is regulated by DUF642 proteins, as described in different plants, including <i>Arabidopsis thaliana</i> and <i>Oryza sativa</i>. Our results demonstrated that heat stress altered the expression of the DUF642 gene, <i>BIIDXI</i>. There was an important decrease in <i>BIIDXI</i> expression during the first hour of HS, followed by an increase at 24 h. <i>bdx</i>-1 seedlings had less tolerance to heat stress but presented a normal heat stress response; <i>HSFA2</i> and <i>HSP22</i> expressions were highly increased, as they were in WT seedlings. Thermopriming triggered changes in pectin methyl esterase activity in WT seedlings, while no increases in PME activity were detected in <i>bdx</i>-1 seedlings at the same conditions. Taken together, our results suggest that BIIDXI is involved in thermotolerance via PME activation.https://www.mdpi.com/2223-7747/11/22/3049plant cell wallthermotoleranceDUF642 proteinspectin methyl esterases |
| spellingShingle | Eduardo Pineda-Hernández José Erik Cruz-Valderrama Ximena Gómez-Maqueo Eleazar Martínez-Barajas Alicia Gamboa-deBuen BIIDXI, a DUF642 Cell Wall Protein That Regulates Pectin Methyl Esterase Activity, Is Involved in Thermotolerance Processes in <i>Arabidopsis thaliana</i> Plants plant cell wall thermotolerance DUF642 proteins pectin methyl esterases |
| title | BIIDXI, a DUF642 Cell Wall Protein That Regulates Pectin Methyl Esterase Activity, Is Involved in Thermotolerance Processes in <i>Arabidopsis thaliana</i> |
| title_full | BIIDXI, a DUF642 Cell Wall Protein That Regulates Pectin Methyl Esterase Activity, Is Involved in Thermotolerance Processes in <i>Arabidopsis thaliana</i> |
| title_fullStr | BIIDXI, a DUF642 Cell Wall Protein That Regulates Pectin Methyl Esterase Activity, Is Involved in Thermotolerance Processes in <i>Arabidopsis thaliana</i> |
| title_full_unstemmed | BIIDXI, a DUF642 Cell Wall Protein That Regulates Pectin Methyl Esterase Activity, Is Involved in Thermotolerance Processes in <i>Arabidopsis thaliana</i> |
| title_short | BIIDXI, a DUF642 Cell Wall Protein That Regulates Pectin Methyl Esterase Activity, Is Involved in Thermotolerance Processes in <i>Arabidopsis thaliana</i> |
| title_sort | biidxi a duf642 cell wall protein that regulates pectin methyl esterase activity is involved in thermotolerance processes in i arabidopsis thaliana i |
| topic | plant cell wall thermotolerance DUF642 proteins pectin methyl esterases |
| url | https://www.mdpi.com/2223-7747/11/22/3049 |
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