Phylogenetic and Functional Diversity of Saprolegniales and Fungi Isolated from Temperate Lakes in Northeast Germany
The contribution of fungi to the degradation of plant litter and transformation of dissolved organic matter (humic substances, in particular) in freshwater ecosystems has received increasing attention recently. However, the role of Saprolegniales as one of the most common eukaryotic organisms is rar...
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MDPI AG
2021-11-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/7/11/968 |
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| author | Hossein Masigol Jason Nicholas Woodhouse Pieter van West Reza Mostowfizadeh-Ghalamfarsa Keilor Rojas-Jimenez Tobias Goldhammer Seyed Akbar Khodaparast Hans-Peter Grossart |
| author_facet | Hossein Masigol Jason Nicholas Woodhouse Pieter van West Reza Mostowfizadeh-Ghalamfarsa Keilor Rojas-Jimenez Tobias Goldhammer Seyed Akbar Khodaparast Hans-Peter Grossart |
| author_sort | Hossein Masigol |
| collection | DOAJ |
| description | The contribution of fungi to the degradation of plant litter and transformation of dissolved organic matter (humic substances, in particular) in freshwater ecosystems has received increasing attention recently. However, the role of Saprolegniales as one of the most common eukaryotic organisms is rarely studied. In this study, we isolated and phylogenetically placed 51 fungal and 62 Saprolegniales strains from 12 German lakes. We studied the cellulo-, lignino-, and chitinolytic activity of the strains using plate assays. Furthermore, we determined the capacity of 10 selected strains to utilize 95 different labile compounds, using Biolog FF MicroPlates™. Finally, the ability of three selected strains to utilize maltose and degrade/produce humic substances was measured. <i>Cladosporium</i> and <i>Penicillium</i> were amongst the most prevalent fungal strains, while <i>Saprolegnia</i>, <i>Achlya</i>, and <i>Leptolegnia</i> were the most frequent Saprolegniales strains. Although the isolated strains assigned to genera were phylogenetically similar, their enzymatic activity and physiological profiling were quite diverse. Our results indicate that Saprolegniales, in contrast to fungi, lack ligninolytic activity and are not involved in the production/transformation of humic substances. We hypothesize that Saprolegniales and fungi might have complementary roles in interacting with dissolved organic matter, which has ecological implications for carbon cycling in freshwater ecosystems. |
| first_indexed | 2024-03-10T05:22:58Z |
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| language | English |
| last_indexed | 2024-03-10T05:22:58Z |
| publishDate | 2021-11-01 |
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| series | Journal of Fungi |
| spelling | doaj.art-c6f8031a5af84e48bd79d8fa7e43dbfc2023-11-22T23:56:23ZengMDPI AGJournal of Fungi2309-608X2021-11-0171196810.3390/jof7110968Phylogenetic and Functional Diversity of Saprolegniales and Fungi Isolated from Temperate Lakes in Northeast GermanyHossein Masigol0Jason Nicholas Woodhouse1Pieter van West2Reza Mostowfizadeh-Ghalamfarsa3Keilor Rojas-Jimenez4Tobias Goldhammer5Seyed Akbar Khodaparast6Hans-Peter Grossart7Experimental Limnology, Leibniz Institute for Freshwater Ecology and Inland Fisheries (IGB), 16775 Neuglobsow, GermanyExperimental Limnology, Leibniz Institute for Freshwater Ecology and Inland Fisheries (IGB), 16775 Neuglobsow, GermanyAberdeen Oomycete Laboratory, International Centre for Aquaculture Research and Development (ICARD), Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen AB25 2ZD, UKDepartment of Plant Protection, School of Agriculture, Shiraz University, Shiraz 71946-84334, IranEscuela de Biologia, Universidad de Costa Rica, San Jose 11501, Costa RicaDepartment of Ecohydrology and Biogeochemistry, Leibniz-Institute of Freshwater Ecology and Inland Fisheries, 12587 Berlin, GermanyDepartment of Plant Protection, Faculty of Agricultural Sciences, University of Guilan, Rasht 41996-13776, IranExperimental Limnology, Leibniz Institute for Freshwater Ecology and Inland Fisheries (IGB), 16775 Neuglobsow, GermanyThe contribution of fungi to the degradation of plant litter and transformation of dissolved organic matter (humic substances, in particular) in freshwater ecosystems has received increasing attention recently. However, the role of Saprolegniales as one of the most common eukaryotic organisms is rarely studied. In this study, we isolated and phylogenetically placed 51 fungal and 62 Saprolegniales strains from 12 German lakes. We studied the cellulo-, lignino-, and chitinolytic activity of the strains using plate assays. Furthermore, we determined the capacity of 10 selected strains to utilize 95 different labile compounds, using Biolog FF MicroPlates™. Finally, the ability of three selected strains to utilize maltose and degrade/produce humic substances was measured. <i>Cladosporium</i> and <i>Penicillium</i> were amongst the most prevalent fungal strains, while <i>Saprolegnia</i>, <i>Achlya</i>, and <i>Leptolegnia</i> were the most frequent Saprolegniales strains. Although the isolated strains assigned to genera were phylogenetically similar, their enzymatic activity and physiological profiling were quite diverse. Our results indicate that Saprolegniales, in contrast to fungi, lack ligninolytic activity and are not involved in the production/transformation of humic substances. We hypothesize that Saprolegniales and fungi might have complementary roles in interacting with dissolved organic matter, which has ecological implications for carbon cycling in freshwater ecosystems.https://www.mdpi.com/2309-608X/7/11/968freshwater ecosystemsaquatic carbon cyclingplant litter degradationhumic substances production<i>Saprolegnia</i><i>Achlya</i> |
| spellingShingle | Hossein Masigol Jason Nicholas Woodhouse Pieter van West Reza Mostowfizadeh-Ghalamfarsa Keilor Rojas-Jimenez Tobias Goldhammer Seyed Akbar Khodaparast Hans-Peter Grossart Phylogenetic and Functional Diversity of Saprolegniales and Fungi Isolated from Temperate Lakes in Northeast Germany Journal of Fungi freshwater ecosystems aquatic carbon cycling plant litter degradation humic substances production <i>Saprolegnia</i> <i>Achlya</i> |
| title | Phylogenetic and Functional Diversity of Saprolegniales and Fungi Isolated from Temperate Lakes in Northeast Germany |
| title_full | Phylogenetic and Functional Diversity of Saprolegniales and Fungi Isolated from Temperate Lakes in Northeast Germany |
| title_fullStr | Phylogenetic and Functional Diversity of Saprolegniales and Fungi Isolated from Temperate Lakes in Northeast Germany |
| title_full_unstemmed | Phylogenetic and Functional Diversity of Saprolegniales and Fungi Isolated from Temperate Lakes in Northeast Germany |
| title_short | Phylogenetic and Functional Diversity of Saprolegniales and Fungi Isolated from Temperate Lakes in Northeast Germany |
| title_sort | phylogenetic and functional diversity of saprolegniales and fungi isolated from temperate lakes in northeast germany |
| topic | freshwater ecosystems aquatic carbon cycling plant litter degradation humic substances production <i>Saprolegnia</i> <i>Achlya</i> |
| url | https://www.mdpi.com/2309-608X/7/11/968 |
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