The yeast telomerase RNA, TLC1, participates in two distinct modes of TLC1-TLC1 association processes in vivo
Telomerase core enzyme minimally consists of the telomerase reverse transcriptase domain-containing protein (Est2 in budding yeast S. cerevisiae) and telomerase RNA, which contains the template specifying the telomeric repeat sequence synthesized. Here we report that in vivo, a fraction of S. cerevi...
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| Format: | Article |
| Language: | English |
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PeerJ Inc.
2016-01-01
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| Online Access: | https://peerj.com/articles/1534.pdf |
| _version_ | 1797424071468646400 |
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| author | Tet Matsuguchi Elizabeth Blackburn |
| author_facet | Tet Matsuguchi Elizabeth Blackburn |
| author_sort | Tet Matsuguchi |
| collection | DOAJ |
| description | Telomerase core enzyme minimally consists of the telomerase reverse transcriptase domain-containing protein (Est2 in budding yeast S. cerevisiae) and telomerase RNA, which contains the template specifying the telomeric repeat sequence synthesized. Here we report that in vivo, a fraction of S. cerevisiae telomerase RNA (TLC1) molecules form complexes containing at least two molecules of TLC1, via two separable modes: one requiring a sequence in the 3′ region of the immature TLC1 precursor and the other requiring Ku and Sir4. Such physical TLC1-TLC1 association peaked in G1 phase and did not require telomere silencing, telomere tethering to the nuclear periphery, telomerase holoenzyme assembly, or detectable Est2-Est2 protein association. These data indicate that TLC1-TLC1 associations reflect processes occurring during telomerase biogenesis; we propose that TLC1-TLC1 associations and subsequent reorganization may be regulatory steps in telomerase enzymatic activation. |
| first_indexed | 2024-03-09T07:56:00Z |
| format | Article |
| id | doaj.art-c6f87d549cc74d619847c817b7505cce |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T07:56:00Z |
| publishDate | 2016-01-01 |
| publisher | PeerJ Inc. |
| record_format | Article |
| series | PeerJ |
| spelling | doaj.art-c6f87d549cc74d619847c817b7505cce2023-12-03T01:00:56ZengPeerJ Inc.PeerJ2167-83592016-01-014e153410.7717/peerj.1534The yeast telomerase RNA, TLC1, participates in two distinct modes of TLC1-TLC1 association processes in vivoTet Matsuguchi0Elizabeth Blackburn1Department of Biochemistry and Biophysics, University of California, San Francisco, CA, United StatesDepartment of Biochemistry and Biophysics, University of California, San Francisco, CA, United StatesTelomerase core enzyme minimally consists of the telomerase reverse transcriptase domain-containing protein (Est2 in budding yeast S. cerevisiae) and telomerase RNA, which contains the template specifying the telomeric repeat sequence synthesized. Here we report that in vivo, a fraction of S. cerevisiae telomerase RNA (TLC1) molecules form complexes containing at least two molecules of TLC1, via two separable modes: one requiring a sequence in the 3′ region of the immature TLC1 precursor and the other requiring Ku and Sir4. Such physical TLC1-TLC1 association peaked in G1 phase and did not require telomere silencing, telomere tethering to the nuclear periphery, telomerase holoenzyme assembly, or detectable Est2-Est2 protein association. These data indicate that TLC1-TLC1 associations reflect processes occurring during telomerase biogenesis; we propose that TLC1-TLC1 associations and subsequent reorganization may be regulatory steps in telomerase enzymatic activation.https://peerj.com/articles/1534.pdfTelomeraseTelomerase RNATLC1BiogenesisYeast |
| spellingShingle | Tet Matsuguchi Elizabeth Blackburn The yeast telomerase RNA, TLC1, participates in two distinct modes of TLC1-TLC1 association processes in vivo PeerJ Telomerase Telomerase RNA TLC1 Biogenesis Yeast |
| title | The yeast telomerase RNA, TLC1, participates in two distinct modes of TLC1-TLC1 association processes in vivo |
| title_full | The yeast telomerase RNA, TLC1, participates in two distinct modes of TLC1-TLC1 association processes in vivo |
| title_fullStr | The yeast telomerase RNA, TLC1, participates in two distinct modes of TLC1-TLC1 association processes in vivo |
| title_full_unstemmed | The yeast telomerase RNA, TLC1, participates in two distinct modes of TLC1-TLC1 association processes in vivo |
| title_short | The yeast telomerase RNA, TLC1, participates in two distinct modes of TLC1-TLC1 association processes in vivo |
| title_sort | yeast telomerase rna tlc1 participates in two distinct modes of tlc1 tlc1 association processes in vivo |
| topic | Telomerase Telomerase RNA TLC1 Biogenesis Yeast |
| url | https://peerj.com/articles/1534.pdf |
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