Bile microbiota in primary sclerosing cholangitis: Impact on disease progression and development of biliary dysplasia.
The etiopathogenesis and risk for development of biliary neoplasia in primary sclerosing cholangitis (PSC) are largely unknown. Microbes or their metabolites have been suggested to play a role. To explore this potential microbial involvement, we evaluated the differences in biliary microbiota in PSC...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2017-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5552186?pdf=render |
| _version_ | 1819063581721755648 |
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| author | Pedro Pereira Velma Aho Johanna Arola Sonja Boyd Kalle Jokelainen Lars Paulin Petri Auvinen Martti Färkkilä |
| author_facet | Pedro Pereira Velma Aho Johanna Arola Sonja Boyd Kalle Jokelainen Lars Paulin Petri Auvinen Martti Färkkilä |
| author_sort | Pedro Pereira |
| collection | DOAJ |
| description | The etiopathogenesis and risk for development of biliary neoplasia in primary sclerosing cholangitis (PSC) are largely unknown. Microbes or their metabolites have been suggested to play a role. To explore this potential microbial involvement, we evaluated the differences in biliary microbiota in PSC patients at an early disease stage without previous endoscopic retrograde cholangiography (ERC) examinations, advanced disease stage, and with biliary dysplasia or cholangiocarcinoma.Bile samples from the common bile duct were collected from 46 controls and 80 patients with PSC during ERC (37 with early disease, 32 with advanced disease, and 11 with biliary dysplasia). DNA isolation, amplification, and Illumina MiSeq sequencing were performed for the V1-V3 regions of the bacterial 16S rRNA gene.The most common phyla found were Bacteroidetes, Firmicutes, Proteobacteria, Fusobacteria, and Actinobacteria. The most common families were Prevotellaceae, Streptococcaceae, Veillonellaceae, Fusobacteriaceae, and Pasteurellaceae, and the most common genera were Prevotella, Streptococcus, Veillonella, Fusobacterium, and Haemophilus. The bacterial communities of non-PSC subjects and early stage PSC patients were similar. Alpha diversity was lower in patients with biliary dysplasia/cholangiocarcinoma than in other groups. An increase in Streptococcus abundance was positively correlated with the number of ERC examinations. Streptococcus abundance was also positively correlated with an increase in disease severity, even after controlling for the number of ERC examinations.Our findings suggest that the aetiology of PSC is not associated with changes in bile microbial communities, but the genus Streptococcus may play a pathogenic role in the progression of the disease. |
| first_indexed | 2024-12-21T15:16:57Z |
| format | Article |
| id | doaj.art-c70064891e0a48f1976f8ea3459359a0 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-21T15:16:57Z |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-c70064891e0a48f1976f8ea3459359a02022-12-21T18:59:08ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01128e018292410.1371/journal.pone.0182924Bile microbiota in primary sclerosing cholangitis: Impact on disease progression and development of biliary dysplasia.Pedro PereiraVelma AhoJohanna ArolaSonja BoydKalle JokelainenLars PaulinPetri AuvinenMartti FärkkiläThe etiopathogenesis and risk for development of biliary neoplasia in primary sclerosing cholangitis (PSC) are largely unknown. Microbes or their metabolites have been suggested to play a role. To explore this potential microbial involvement, we evaluated the differences in biliary microbiota in PSC patients at an early disease stage without previous endoscopic retrograde cholangiography (ERC) examinations, advanced disease stage, and with biliary dysplasia or cholangiocarcinoma.Bile samples from the common bile duct were collected from 46 controls and 80 patients with PSC during ERC (37 with early disease, 32 with advanced disease, and 11 with biliary dysplasia). DNA isolation, amplification, and Illumina MiSeq sequencing were performed for the V1-V3 regions of the bacterial 16S rRNA gene.The most common phyla found were Bacteroidetes, Firmicutes, Proteobacteria, Fusobacteria, and Actinobacteria. The most common families were Prevotellaceae, Streptococcaceae, Veillonellaceae, Fusobacteriaceae, and Pasteurellaceae, and the most common genera were Prevotella, Streptococcus, Veillonella, Fusobacterium, and Haemophilus. The bacterial communities of non-PSC subjects and early stage PSC patients were similar. Alpha diversity was lower in patients with biliary dysplasia/cholangiocarcinoma than in other groups. An increase in Streptococcus abundance was positively correlated with the number of ERC examinations. Streptococcus abundance was also positively correlated with an increase in disease severity, even after controlling for the number of ERC examinations.Our findings suggest that the aetiology of PSC is not associated with changes in bile microbial communities, but the genus Streptococcus may play a pathogenic role in the progression of the disease.http://europepmc.org/articles/PMC5552186?pdf=render |
| spellingShingle | Pedro Pereira Velma Aho Johanna Arola Sonja Boyd Kalle Jokelainen Lars Paulin Petri Auvinen Martti Färkkilä Bile microbiota in primary sclerosing cholangitis: Impact on disease progression and development of biliary dysplasia. PLoS ONE |
| title | Bile microbiota in primary sclerosing cholangitis: Impact on disease progression and development of biliary dysplasia. |
| title_full | Bile microbiota in primary sclerosing cholangitis: Impact on disease progression and development of biliary dysplasia. |
| title_fullStr | Bile microbiota in primary sclerosing cholangitis: Impact on disease progression and development of biliary dysplasia. |
| title_full_unstemmed | Bile microbiota in primary sclerosing cholangitis: Impact on disease progression and development of biliary dysplasia. |
| title_short | Bile microbiota in primary sclerosing cholangitis: Impact on disease progression and development of biliary dysplasia. |
| title_sort | bile microbiota in primary sclerosing cholangitis impact on disease progression and development of biliary dysplasia |
| url | http://europepmc.org/articles/PMC5552186?pdf=render |
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