Distinct and Overlapping Patterns of Acute Ethanol-Induced C-Fos Activation in Two Inbred Replicate Lines of Mice Selected for Drinking to High Blood Ethanol Concentrations
The inbred high drinking in the dark (iHDID1 and iHDID2) strains are two replicate lines bred from the parent HS/Npt (HS) line for achieving binge levels of blood ethanol concentration (≥80 mg/dL BEC) in a four-hour period. In this work, we sought to evaluate differences in baseline and ethanol-indu...
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| Language: | English |
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MDPI AG
2020-12-01
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| Series: | Brain Sciences |
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| Online Access: | https://www.mdpi.com/2076-3425/10/12/988 |
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| author | Stacey L. Robinson Ana Paula S. Dornellas Nathan W. Burnham Christa A. Houck Kendall L. Luhn Sophie C. Bendrath Michel A. Companion Honoreé W. Brewton Rhiannon D. Thomas Montserrat Navarro Todd E. Thiele |
| author_facet | Stacey L. Robinson Ana Paula S. Dornellas Nathan W. Burnham Christa A. Houck Kendall L. Luhn Sophie C. Bendrath Michel A. Companion Honoreé W. Brewton Rhiannon D. Thomas Montserrat Navarro Todd E. Thiele |
| author_sort | Stacey L. Robinson |
| collection | DOAJ |
| description | The inbred high drinking in the dark (iHDID1 and iHDID2) strains are two replicate lines bred from the parent HS/Npt (HS) line for achieving binge levels of blood ethanol concentration (≥80 mg/dL BEC) in a four-hour period. In this work, we sought to evaluate differences in baseline and ethanol-induced c-Fos activation between the HS, iHDID1, and iHDID2 genetic lines in brain regions known to process the aversive properties of ethanol. Methods: Male and female HS, iHDID1, and iHDID2 mice underwent an IP saline 2 3 g/kg ethanol injection. Brain sections were then stained for c-Fos expression in the basolateral/central amygdala (BLA/CeA), bed nucleus of the stria terminals (BNST), A2, locus coeruleus (LC), parabrachial nucleus (PBN), lateral/medial habenula (LHb/MHb), paraventricular nucleus of the thalamus (PVT), periaqueductal gray (PAG), Edinger–Westphal nuclei (EW), and rostromedial tegmental nucleus (RMTg). Results: The iHDID1 and iHDID2 lines showed similar and distinct patterns of regional c-Fos; however, in no region did the two both significantly differ from the HS line together. Conclusions: Our findings lend further support to the hypothesis the iHDID1 and the iHDID2 lines arrive at a similar behavior phenotype through divergent genetic mechanisms. |
| first_indexed | 2024-03-10T14:04:16Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2076-3425 |
| language | English |
| last_indexed | 2024-03-10T14:04:16Z |
| publishDate | 2020-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Brain Sciences |
| spelling | doaj.art-c706e11dbfeb4f3c90e8dd276730ca562023-11-21T00:52:50ZengMDPI AGBrain Sciences2076-34252020-12-01101298810.3390/brainsci10120988Distinct and Overlapping Patterns of Acute Ethanol-Induced C-Fos Activation in Two Inbred Replicate Lines of Mice Selected for Drinking to High Blood Ethanol ConcentrationsStacey L. Robinson0Ana Paula S. Dornellas1Nathan W. Burnham2Christa A. Houck3Kendall L. Luhn4Sophie C. Bendrath5Michel A. Companion6Honoreé W. Brewton7Rhiannon D. Thomas8Montserrat Navarro9Todd E. Thiele10Department of Psychology & Neuroscience, The University of North Carolina, Chapel Hill, NC 27599, USADepartment of Psychology & Neuroscience, The University of North Carolina, Chapel Hill, NC 27599, USADepartment of Chemistry, North Carolina State University, Raleigh, NC 27695, USADepartment of Psychology & Neuroscience, The University of North Carolina, Chapel Hill, NC 27599, USADepartment of Psychology & Neuroscience, The University of North Carolina, Chapel Hill, NC 27599, USADepartment of Psychology & Neuroscience, The University of North Carolina, Chapel Hill, NC 27599, USADepartment of Psychology & Neuroscience, The University of North Carolina, Chapel Hill, NC 27599, USADepartment of Psychology & Neuroscience, The University of North Carolina, Chapel Hill, NC 27599, USADepartment of Psychology & Neuroscience, The University of North Carolina, Chapel Hill, NC 27599, USADepartment of Psychology & Neuroscience, The University of North Carolina, Chapel Hill, NC 27599, USADepartment of Psychology & Neuroscience, The University of North Carolina, Chapel Hill, NC 27599, USAThe inbred high drinking in the dark (iHDID1 and iHDID2) strains are two replicate lines bred from the parent HS/Npt (HS) line for achieving binge levels of blood ethanol concentration (≥80 mg/dL BEC) in a four-hour period. In this work, we sought to evaluate differences in baseline and ethanol-induced c-Fos activation between the HS, iHDID1, and iHDID2 genetic lines in brain regions known to process the aversive properties of ethanol. Methods: Male and female HS, iHDID1, and iHDID2 mice underwent an IP saline 2 3 g/kg ethanol injection. Brain sections were then stained for c-Fos expression in the basolateral/central amygdala (BLA/CeA), bed nucleus of the stria terminals (BNST), A2, locus coeruleus (LC), parabrachial nucleus (PBN), lateral/medial habenula (LHb/MHb), paraventricular nucleus of the thalamus (PVT), periaqueductal gray (PAG), Edinger–Westphal nuclei (EW), and rostromedial tegmental nucleus (RMTg). Results: The iHDID1 and iHDID2 lines showed similar and distinct patterns of regional c-Fos; however, in no region did the two both significantly differ from the HS line together. Conclusions: Our findings lend further support to the hypothesis the iHDID1 and the iHDID2 lines arrive at a similar behavior phenotype through divergent genetic mechanisms.https://www.mdpi.com/2076-3425/10/12/988HDIDc-fosmiceethanolalcoholdrinking in the dark |
| spellingShingle | Stacey L. Robinson Ana Paula S. Dornellas Nathan W. Burnham Christa A. Houck Kendall L. Luhn Sophie C. Bendrath Michel A. Companion Honoreé W. Brewton Rhiannon D. Thomas Montserrat Navarro Todd E. Thiele Distinct and Overlapping Patterns of Acute Ethanol-Induced C-Fos Activation in Two Inbred Replicate Lines of Mice Selected for Drinking to High Blood Ethanol Concentrations Brain Sciences HDID c-fos mice ethanol alcohol drinking in the dark |
| title | Distinct and Overlapping Patterns of Acute Ethanol-Induced C-Fos Activation in Two Inbred Replicate Lines of Mice Selected for Drinking to High Blood Ethanol Concentrations |
| title_full | Distinct and Overlapping Patterns of Acute Ethanol-Induced C-Fos Activation in Two Inbred Replicate Lines of Mice Selected for Drinking to High Blood Ethanol Concentrations |
| title_fullStr | Distinct and Overlapping Patterns of Acute Ethanol-Induced C-Fos Activation in Two Inbred Replicate Lines of Mice Selected for Drinking to High Blood Ethanol Concentrations |
| title_full_unstemmed | Distinct and Overlapping Patterns of Acute Ethanol-Induced C-Fos Activation in Two Inbred Replicate Lines of Mice Selected for Drinking to High Blood Ethanol Concentrations |
| title_short | Distinct and Overlapping Patterns of Acute Ethanol-Induced C-Fos Activation in Two Inbred Replicate Lines of Mice Selected for Drinking to High Blood Ethanol Concentrations |
| title_sort | distinct and overlapping patterns of acute ethanol induced c fos activation in two inbred replicate lines of mice selected for drinking to high blood ethanol concentrations |
| topic | HDID c-fos mice ethanol alcohol drinking in the dark |
| url | https://www.mdpi.com/2076-3425/10/12/988 |
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