Pathological Properties of Cryptococcus pseudolongus on the Mycelia and Fruit Body of Lentinula edodes
Recently, Cryptococcus pseudolongus has been reported as a new pathogen of shiitake (Lentinula edodes). However, its pathological properties are not much known. To further understand its impact on the mushroom, we investigated the pathogen’s interactions with the mycelium of shiitake, histopathologi...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2021-03-01
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| Series: | Mycobiology |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/12298093.2021.1873486 |
| _version_ | 1818443400708358144 |
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| author | Hyuk Woo Kwon Seong Hwan Kim |
| author_facet | Hyuk Woo Kwon Seong Hwan Kim |
| author_sort | Hyuk Woo Kwon |
| collection | DOAJ |
| description | Recently, Cryptococcus pseudolongus has been reported as a new pathogen of shiitake (Lentinula edodes). However, its pathological properties are not much known. To further understand its impact on the mushroom, we investigated the pathogen’s interactions with the mycelium of shiitake, histopathological properties, host range, and sensitivity to diverse antifungal agents. The strain C. pseudolongus DUCC 4014 inhibited the mycelial growth of L. edodes strain (cultivar Sanjo 701ho) and caused browning in the mycelia confronted with the yeast on PDA. Spray inoculation of the yeast caused an abnormal browning symptom on the cap and/or gills of three shiitake cultivars grown on sawdust media in vinyl bags. Scanning electron microscopic images of the abnormally browned parts of shiitake fruit body illustrated that mushroom tissues were loosed and dispersed in the middle and edge of the cap and the arrangement of basidiospores borne on basidia in the gills was disturbed compared to those of normal shiitake fruit body. Spray inoculation also led to developing abnormal browning on the harvested fruit body, indicating C. pseudolongus could be a problem during mushroom storage. But the yeast was not able to induce abnormal browning on mushrooms of Pleurotus ferulae, Pleurotus fostreatus, and Agaricus bisporus. But it induced browning only on button mushroom (A. bisporus) when they were inoculated after wounding. Tests with 16 kinds of fungicides revealed that the cell growth of C. pseudolongus could be inhibited by benzalkonium chloride at MIC 7 μg/ml and benomyl at MIC 3 μg/ml. |
| first_indexed | 2024-12-14T18:59:27Z |
| format | Article |
| id | doaj.art-c709c927bd224bc9afb4765167285cf8 |
| institution | Directory Open Access Journal |
| issn | 1229-8093 2092-9323 |
| language | English |
| last_indexed | 2024-12-14T18:59:27Z |
| publishDate | 2021-03-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Mycobiology |
| spelling | doaj.art-c709c927bd224bc9afb4765167285cf82022-12-21T22:51:00ZengTaylor & Francis GroupMycobiology1229-80932092-93232021-03-0149217318210.1080/12298093.2021.18734861873486Pathological Properties of Cryptococcus pseudolongus on the Mycelia and Fruit Body of Lentinula edodesHyuk Woo Kwon0Seong Hwan Kim1Forest Mushroom Research Center, National Forestry Cooperative FederationDepartment of Microbiology and Institute of Biodiversity, Dankook UniversityRecently, Cryptococcus pseudolongus has been reported as a new pathogen of shiitake (Lentinula edodes). However, its pathological properties are not much known. To further understand its impact on the mushroom, we investigated the pathogen’s interactions with the mycelium of shiitake, histopathological properties, host range, and sensitivity to diverse antifungal agents. The strain C. pseudolongus DUCC 4014 inhibited the mycelial growth of L. edodes strain (cultivar Sanjo 701ho) and caused browning in the mycelia confronted with the yeast on PDA. Spray inoculation of the yeast caused an abnormal browning symptom on the cap and/or gills of three shiitake cultivars grown on sawdust media in vinyl bags. Scanning electron microscopic images of the abnormally browned parts of shiitake fruit body illustrated that mushroom tissues were loosed and dispersed in the middle and edge of the cap and the arrangement of basidiospores borne on basidia in the gills was disturbed compared to those of normal shiitake fruit body. Spray inoculation also led to developing abnormal browning on the harvested fruit body, indicating C. pseudolongus could be a problem during mushroom storage. But the yeast was not able to induce abnormal browning on mushrooms of Pleurotus ferulae, Pleurotus fostreatus, and Agaricus bisporus. But it induced browning only on button mushroom (A. bisporus) when they were inoculated after wounding. Tests with 16 kinds of fungicides revealed that the cell growth of C. pseudolongus could be inhibited by benzalkonium chloride at MIC 7 μg/ml and benomyl at MIC 3 μg/ml.http://dx.doi.org/10.1080/12298093.2021.1873486brown rotcryptococcus pseudolongusshiitake; lentinula edodes |
| spellingShingle | Hyuk Woo Kwon Seong Hwan Kim Pathological Properties of Cryptococcus pseudolongus on the Mycelia and Fruit Body of Lentinula edodes Mycobiology brown rot cryptococcus pseudolongus shiitake; lentinula edodes |
| title | Pathological Properties of Cryptococcus pseudolongus on the Mycelia and Fruit Body of Lentinula edodes |
| title_full | Pathological Properties of Cryptococcus pseudolongus on the Mycelia and Fruit Body of Lentinula edodes |
| title_fullStr | Pathological Properties of Cryptococcus pseudolongus on the Mycelia and Fruit Body of Lentinula edodes |
| title_full_unstemmed | Pathological Properties of Cryptococcus pseudolongus on the Mycelia and Fruit Body of Lentinula edodes |
| title_short | Pathological Properties of Cryptococcus pseudolongus on the Mycelia and Fruit Body of Lentinula edodes |
| title_sort | pathological properties of cryptococcus pseudolongus on the mycelia and fruit body of lentinula edodes |
| topic | brown rot cryptococcus pseudolongus shiitake; lentinula edodes |
| url | http://dx.doi.org/10.1080/12298093.2021.1873486 |
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