Usage of DNA Fingerprinting Technology to Check Sample Error and Contamination in Molecular Laboratories
Sample identification error is a severe medical error in clinical molecular diagnostic laboratories, which can lead to reporting the wrong results for the patient involved. Sample contamination can also lead to incorrect test reports. Avoiding sample identification error and sample contamination cou...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-11-01
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| Series: | Current Issues in Molecular Biology |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1467-3045/44/11/375 |
| _version_ | 1827646960663789568 |
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| author | Dahui Qin Mark Forster Shital M. Gandhi Ratilal Akabari Zhong Zheng Janella Lal Katherine Lovinger |
| author_facet | Dahui Qin Mark Forster Shital M. Gandhi Ratilal Akabari Zhong Zheng Janella Lal Katherine Lovinger |
| author_sort | Dahui Qin |
| collection | DOAJ |
| description | Sample identification error is a severe medical error in clinical molecular diagnostic laboratories, which can lead to reporting the wrong results for the patient involved. Sample contamination can also lead to incorrect test reports. Avoiding sample identification error and sample contamination could be life-saving. Sample switch and sample contamination could happen on laboratory bench works, especially when pipetting into multi-well plates. It is difficult to realize such errors during laboratory bench work. Laboratory staff may not be aware of such an error when it happens. DNA fingerprinting technology can be used to determine sample identity and subsequently identify sample switch and sample contamination in the laboratory. Our laboratory has explored the usage of this technology in our quality control process and successfully established that DNA fingerprinting can be used to monitor sample switch and sample contamination in next-generation sequencing and BCR/ABL1 real-time PCR bench work. |
| first_indexed | 2024-03-09T19:10:41Z |
| format | Article |
| id | doaj.art-c70b3aa4d50d4e2b927037cd27e2ae4c |
| institution | Directory Open Access Journal |
| issn | 1467-3037 1467-3045 |
| language | English |
| last_indexed | 2024-03-09T19:10:41Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Current Issues in Molecular Biology |
| spelling | doaj.art-c70b3aa4d50d4e2b927037cd27e2ae4c2023-11-24T04:13:20ZengMDPI AGCurrent Issues in Molecular Biology1467-30371467-30452022-11-0144115543554910.3390/cimb44110375Usage of DNA Fingerprinting Technology to Check Sample Error and Contamination in Molecular LaboratoriesDahui Qin0Mark Forster1Shital M. Gandhi2Ratilal Akabari3Zhong Zheng4Janella Lal5Katherine Lovinger6Molecular Diagnostic Laboratory, Moffitt Cancer Center, Tampa, FL 33612, USAMolecular Diagnostic Laboratory, Moffitt Cancer Center, Tampa, FL 33612, USAMolecular Diagnostic Laboratory, Moffitt Cancer Center, Tampa, FL 33612, USAMolecular Diagnostic Laboratory, State University of New York (SUNY), Upstate Medical University, Syracuse, NY 13210, USAMolecular Diagnostic Laboratory, Moffitt Cancer Center, Tampa, FL 33612, USAMolecular Diagnostic Laboratory, Emory University Hospital, Atlanta, GA 30322, USAOrlando Health (ORMC’s) Molecular Laboratory, Orlando, FL 32806, USASample identification error is a severe medical error in clinical molecular diagnostic laboratories, which can lead to reporting the wrong results for the patient involved. Sample contamination can also lead to incorrect test reports. Avoiding sample identification error and sample contamination could be life-saving. Sample switch and sample contamination could happen on laboratory bench works, especially when pipetting into multi-well plates. It is difficult to realize such errors during laboratory bench work. Laboratory staff may not be aware of such an error when it happens. DNA fingerprinting technology can be used to determine sample identity and subsequently identify sample switch and sample contamination in the laboratory. Our laboratory has explored the usage of this technology in our quality control process and successfully established that DNA fingerprinting can be used to monitor sample switch and sample contamination in next-generation sequencing and BCR/ABL1 real-time PCR bench work.https://www.mdpi.com/1467-3045/44/11/375quality controlsample switchsample contaminationsample errorDNA fingerprint |
| spellingShingle | Dahui Qin Mark Forster Shital M. Gandhi Ratilal Akabari Zhong Zheng Janella Lal Katherine Lovinger Usage of DNA Fingerprinting Technology to Check Sample Error and Contamination in Molecular Laboratories Current Issues in Molecular Biology quality control sample switch sample contamination sample error DNA fingerprint |
| title | Usage of DNA Fingerprinting Technology to Check Sample Error and Contamination in Molecular Laboratories |
| title_full | Usage of DNA Fingerprinting Technology to Check Sample Error and Contamination in Molecular Laboratories |
| title_fullStr | Usage of DNA Fingerprinting Technology to Check Sample Error and Contamination in Molecular Laboratories |
| title_full_unstemmed | Usage of DNA Fingerprinting Technology to Check Sample Error and Contamination in Molecular Laboratories |
| title_short | Usage of DNA Fingerprinting Technology to Check Sample Error and Contamination in Molecular Laboratories |
| title_sort | usage of dna fingerprinting technology to check sample error and contamination in molecular laboratories |
| topic | quality control sample switch sample contamination sample error DNA fingerprint |
| url | https://www.mdpi.com/1467-3045/44/11/375 |
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