Cytomorphometric and Clinical Changes in Gingival Tissue after Subgingival Tooth Preparation—A Pilot Study
Tooth preparation for a metal-ceramic crown with a subgingival finish line can lead to inflammatory changes in the gingival tissue, often accompanied by cell damage. This study aimed to evaluate the clinical signs of inflammation and the cytomorphological status of the gingival tissue before and aft...
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| Format: | Article |
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MDPI AG
2023-02-01
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| Online Access: | https://www.mdpi.com/2227-9032/11/3/414 |
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| author | Marija Jovanovic Nikola Zivkovic Nikola Gligorijevic Marko Igic Milica Petrovic Marija Bojovic Rodoljub Jovanovic Milena Kostic |
| author_facet | Marija Jovanovic Nikola Zivkovic Nikola Gligorijevic Marko Igic Milica Petrovic Marija Bojovic Rodoljub Jovanovic Milena Kostic |
| author_sort | Marija Jovanovic |
| collection | DOAJ |
| description | Tooth preparation for a metal-ceramic crown with a subgingival finish line can lead to inflammatory changes in the gingival tissue, often accompanied by cell damage. This study aimed to evaluate the clinical signs of inflammation and the cytomorphological status of the gingival tissue before and after tooth preparation. The research included a homogeneous group of 19 patients with an indication for upper canine preparation. Before and after treatment, the gingival and the gingival bleeding indexes were determined, gingival swabs were taken, and direct smears prepared on slides for cytomorphometric analysis. The values of the measured gingival indexes were statistically significantly higher (<i>p</i> < 0.001) after tooth preparation. They decreased over time, which indicated the reversibility of the resulting changes. Cytological examination showed no statistically significant difference between the values of nuclear area, perimeter, Feret diameter, Feret angle, integrated optical density, MinFeret, and roundness, before and after the treatment. Significantly higher values of circularity, integrated optical density, MinFeret (<i>p</i> < 0.05), as well as roundness (<i>p</i> < 0.001) were found after 72 h, compared to those taken 15 min after tooth crown preparation. This study is a pioneering attempt to show gingival changes during fixed prosthodontic treatment and may shed new light on pathogenetic events in prosthodontic patients. |
| first_indexed | 2024-03-11T09:44:17Z |
| format | Article |
| id | doaj.art-c70cc04bbde443138ff4a3fb3644bef3 |
| institution | Directory Open Access Journal |
| issn | 2227-9032 |
| language | English |
| last_indexed | 2024-03-11T09:44:17Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Healthcare |
| spelling | doaj.art-c70cc04bbde443138ff4a3fb3644bef32023-11-16T16:45:03ZengMDPI AGHealthcare2227-90322023-02-0111341410.3390/healthcare11030414Cytomorphometric and Clinical Changes in Gingival Tissue after Subgingival Tooth Preparation—A Pilot StudyMarija Jovanovic0Nikola Zivkovic1Nikola Gligorijevic2Marko Igic3Milica Petrovic4Marija Bojovic5Rodoljub Jovanovic6Milena Kostic7Department of Prosthodontics, Faculty of Medicine, University of Niš, 18000 Niš, SerbiaDepartment of Pathology, Faculty of Medicine, University of Niš, 18000 Niš, SerbiaDepartment of Prosthodontics, Faculty of Medicine, University of Niš, 18000 Niš, SerbiaDepartment of Prosthodontics, Faculty of Medicine, University of Niš, 18000 Niš, SerbiaDepartment of Oral Medicine and Periodontology, Faculty of Medicine, University of Niš, 18000 Niš, SerbiaDepartment of Oral Medicine and Periodontology, Faculty of Medicine, University of Niš, 18000 Niš, SerbiaFaculty of Medicine, University of Niš, 18000 Niš, SerbiaDepartment of Prosthodontics, Faculty of Medicine, University of Niš, 18000 Niš, SerbiaTooth preparation for a metal-ceramic crown with a subgingival finish line can lead to inflammatory changes in the gingival tissue, often accompanied by cell damage. This study aimed to evaluate the clinical signs of inflammation and the cytomorphological status of the gingival tissue before and after tooth preparation. The research included a homogeneous group of 19 patients with an indication for upper canine preparation. Before and after treatment, the gingival and the gingival bleeding indexes were determined, gingival swabs were taken, and direct smears prepared on slides for cytomorphometric analysis. The values of the measured gingival indexes were statistically significantly higher (<i>p</i> < 0.001) after tooth preparation. They decreased over time, which indicated the reversibility of the resulting changes. Cytological examination showed no statistically significant difference between the values of nuclear area, perimeter, Feret diameter, Feret angle, integrated optical density, MinFeret, and roundness, before and after the treatment. Significantly higher values of circularity, integrated optical density, MinFeret (<i>p</i> < 0.05), as well as roundness (<i>p</i> < 0.001) were found after 72 h, compared to those taken 15 min after tooth crown preparation. This study is a pioneering attempt to show gingival changes during fixed prosthodontic treatment and may shed new light on pathogenetic events in prosthodontic patients.https://www.mdpi.com/2227-9032/11/3/414fixed prosthodontic restorationsgingival indexgingival bleeding indexcytomorphometry |
| spellingShingle | Marija Jovanovic Nikola Zivkovic Nikola Gligorijevic Marko Igic Milica Petrovic Marija Bojovic Rodoljub Jovanovic Milena Kostic Cytomorphometric and Clinical Changes in Gingival Tissue after Subgingival Tooth Preparation—A Pilot Study Healthcare fixed prosthodontic restorations gingival index gingival bleeding index cytomorphometry |
| title | Cytomorphometric and Clinical Changes in Gingival Tissue after Subgingival Tooth Preparation—A Pilot Study |
| title_full | Cytomorphometric and Clinical Changes in Gingival Tissue after Subgingival Tooth Preparation—A Pilot Study |
| title_fullStr | Cytomorphometric and Clinical Changes in Gingival Tissue after Subgingival Tooth Preparation—A Pilot Study |
| title_full_unstemmed | Cytomorphometric and Clinical Changes in Gingival Tissue after Subgingival Tooth Preparation—A Pilot Study |
| title_short | Cytomorphometric and Clinical Changes in Gingival Tissue after Subgingival Tooth Preparation—A Pilot Study |
| title_sort | cytomorphometric and clinical changes in gingival tissue after subgingival tooth preparation a pilot study |
| topic | fixed prosthodontic restorations gingival index gingival bleeding index cytomorphometry |
| url | https://www.mdpi.com/2227-9032/11/3/414 |
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