Quantitative Proteomics of Polarised Macrophages Derived from Induced Pluripotent Stem Cells
Macrophages (M<sub>Φ</sub>) are highly heterogenous and versatile innate immune cells involved in homeostatic and immune responses. Activated M<sub>Φ</sub> can exist in two extreme phenotypes: pro-inflammatory (M1) M<sub>Φ</sub> and anti-inflammatory (M2) M<sub...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-01-01
|
| Series: | Biomedicines |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2227-9059/10/2/239 |
| _version_ | 1797482496894435328 |
|---|---|
| author | Gavuthami Murugesan Lindsay Davidson Linda Jannetti Paul R. Crocker Bernd Weigle |
| author_facet | Gavuthami Murugesan Lindsay Davidson Linda Jannetti Paul R. Crocker Bernd Weigle |
| author_sort | Gavuthami Murugesan |
| collection | DOAJ |
| description | Macrophages (M<sub>Φ</sub>) are highly heterogenous and versatile innate immune cells involved in homeostatic and immune responses. Activated M<sub>Φ</sub> can exist in two extreme phenotypes: pro-inflammatory (M1) M<sub>Φ</sub> and anti-inflammatory (M2) M<sub>Φ</sub>. These phenotypes can be recapitulated in vitro by using ligands of toll-like receptors (TLRs) and cytokines such as IFNγ and IL-4. In recent years, human induced pluripotent stem cells (iPSC)-derived M<sub>Φ</sub> have gained major attention, as they are functionally similar to human monocyte-derived M<sub>Φ</sub> and are receptive to genome editing. In this study, we polarised iPSC-derived M<sub>Φ</sub> to M1 or M2 and analysed their proteome and secretome profiles using quantitative proteomics. These comprehensive proteomic data sets provide new insights into functions of polarised M<sub>Φ</sub>. |
| first_indexed | 2024-03-09T22:34:09Z |
| format | Article |
| id | doaj.art-c70f6aaf21454edf8eec055cbc14c474 |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-09T22:34:09Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-c70f6aaf21454edf8eec055cbc14c4742023-11-23T18:52:29ZengMDPI AGBiomedicines2227-90592022-01-0110223910.3390/biomedicines10020239Quantitative Proteomics of Polarised Macrophages Derived from Induced Pluripotent Stem CellsGavuthami Murugesan0Lindsay Davidson1Linda Jannetti2Paul R. Crocker3Bernd Weigle4Division of Cell Signalling and Immunology, School of Life Sciences, University of Dundee, Dundee DD1 5EH, UKHuman Pluripotent Stem Cell Facility, School of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5EH, UKDivision of Cancer Immunology and Immune Modulation, Boehringer Ingelheim Pharma GmbH & Co. KG, 88397 Biberach an der Riss, GermanyDivision of Cell Signalling and Immunology, School of Life Sciences, University of Dundee, Dundee DD1 5EH, UKDivision of Cancer Immunology and Immune Modulation, Boehringer Ingelheim Pharma GmbH & Co. KG, 88397 Biberach an der Riss, GermanyMacrophages (M<sub>Φ</sub>) are highly heterogenous and versatile innate immune cells involved in homeostatic and immune responses. Activated M<sub>Φ</sub> can exist in two extreme phenotypes: pro-inflammatory (M1) M<sub>Φ</sub> and anti-inflammatory (M2) M<sub>Φ</sub>. These phenotypes can be recapitulated in vitro by using ligands of toll-like receptors (TLRs) and cytokines such as IFNγ and IL-4. In recent years, human induced pluripotent stem cells (iPSC)-derived M<sub>Φ</sub> have gained major attention, as they are functionally similar to human monocyte-derived M<sub>Φ</sub> and are receptive to genome editing. In this study, we polarised iPSC-derived M<sub>Φ</sub> to M1 or M2 and analysed their proteome and secretome profiles using quantitative proteomics. These comprehensive proteomic data sets provide new insights into functions of polarised M<sub>Φ</sub>.https://www.mdpi.com/2227-9059/10/2/239iPSCmacrophagespolarisationsecretomeproteomics |
| spellingShingle | Gavuthami Murugesan Lindsay Davidson Linda Jannetti Paul R. Crocker Bernd Weigle Quantitative Proteomics of Polarised Macrophages Derived from Induced Pluripotent Stem Cells Biomedicines iPSC macrophages polarisation secretome proteomics |
| title | Quantitative Proteomics of Polarised Macrophages Derived from Induced Pluripotent Stem Cells |
| title_full | Quantitative Proteomics of Polarised Macrophages Derived from Induced Pluripotent Stem Cells |
| title_fullStr | Quantitative Proteomics of Polarised Macrophages Derived from Induced Pluripotent Stem Cells |
| title_full_unstemmed | Quantitative Proteomics of Polarised Macrophages Derived from Induced Pluripotent Stem Cells |
| title_short | Quantitative Proteomics of Polarised Macrophages Derived from Induced Pluripotent Stem Cells |
| title_sort | quantitative proteomics of polarised macrophages derived from induced pluripotent stem cells |
| topic | iPSC macrophages polarisation secretome proteomics |
| url | https://www.mdpi.com/2227-9059/10/2/239 |
| work_keys_str_mv | AT gavuthamimurugesan quantitativeproteomicsofpolarisedmacrophagesderivedfrominducedpluripotentstemcells AT lindsaydavidson quantitativeproteomicsofpolarisedmacrophagesderivedfrominducedpluripotentstemcells AT lindajannetti quantitativeproteomicsofpolarisedmacrophagesderivedfrominducedpluripotentstemcells AT paulrcrocker quantitativeproteomicsofpolarisedmacrophagesderivedfrominducedpluripotentstemcells AT berndweigle quantitativeproteomicsofpolarisedmacrophagesderivedfrominducedpluripotentstemcells |