Ameliorative effects of canolol against acrylamide toxicity in PC12 cells through modulating MAPKs pathway and autophagy
Acrylamide (ACR), widely present in heat treatment of starchy foods, has attracted much attention in chronic neurotoxin. Canolol is a crucial phenolic compound and generated in rapeseed oil during microwave pressing. The present study aimed to reveal the ameliorative effects of canolol against ACR-i...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2020-12-01
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| Series: | Journal of Functional Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464620304813 |
| _version_ | 1819292805707595776 |
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| author | Xiaoyang Xia Zhen Zhang Chang Zheng Qianchun Deng Mingming Zheng Ling Han Xia Xiang |
| author_facet | Xiaoyang Xia Zhen Zhang Chang Zheng Qianchun Deng Mingming Zheng Ling Han Xia Xiang |
| author_sort | Xiaoyang Xia |
| collection | DOAJ |
| description | Acrylamide (ACR), widely present in heat treatment of starchy foods, has attracted much attention in chronic neurotoxin. Canolol is a crucial phenolic compound and generated in rapeseed oil during microwave pressing. The present study aimed to reveal the ameliorative effects of canolol against ACR-induced toxicity in PC12 cells and the underlying mechanism. Cells pretreated with canolol inhibited ACR-exerted cytotoxicity and cell apoptosis. Canolol attenuated the increase of intracellular ROS and MDA level induced by ACR, and restored ACR-triggered depletion of glutathione content and mitochondrial membrane potential. Furthermore, canolol upregulated expressions of phosphorylated ERK1/2 protein, and downregulated phosphorylated p38 and JNK proteins after 24 h pretreatment in ACR-treated PC12 cells. Notably, ACR-stimulated autophagy activation was hindered by canolol in counteracting ACR-exerted LC-3 conversion and promoting the expression of p62 protein. Our study demonstrated that canolol prevented ACR damage involving MAPKs pathway and autophagy in PC12 cells, providing a potential application of canolol for ACR toxicity. |
| first_indexed | 2024-12-24T04:00:22Z |
| format | Article |
| id | doaj.art-c712c02eb19f441f84b93b769fc3ee1d |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-12-24T04:00:22Z |
| publishDate | 2020-12-01 |
| publisher | Elsevier |
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| series | Journal of Functional Foods |
| spelling | doaj.art-c712c02eb19f441f84b93b769fc3ee1d2022-12-21T17:16:19ZengElsevierJournal of Functional Foods1756-46462020-12-0175104257Ameliorative effects of canolol against acrylamide toxicity in PC12 cells through modulating MAPKs pathway and autophagyXiaoyang Xia0Zhen Zhang1Chang Zheng2Qianchun Deng3Mingming Zheng4Ling Han5Xia Xiang6Oil Crops and Lipids Process Technology National & Local Joint Engineering Laboratory, Key Laboratory of Oilseeds processing, Ministry of Agriculture and Rural Affairs, Hubei Key Laboratory of Lipid Chemistry and Nutrition, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, ChinaSchool of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, ChinaOil Crops and Lipids Process Technology National & Local Joint Engineering Laboratory, Key Laboratory of Oilseeds processing, Ministry of Agriculture and Rural Affairs, Hubei Key Laboratory of Lipid Chemistry and Nutrition, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, ChinaOil Crops and Lipids Process Technology National & Local Joint Engineering Laboratory, Key Laboratory of Oilseeds processing, Ministry of Agriculture and Rural Affairs, Hubei Key Laboratory of Lipid Chemistry and Nutrition, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, ChinaOil Crops and Lipids Process Technology National & Local Joint Engineering Laboratory, Key Laboratory of Oilseeds processing, Ministry of Agriculture and Rural Affairs, Hubei Key Laboratory of Lipid Chemistry and Nutrition, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, ChinaOil Crops and Lipids Process Technology National & Local Joint Engineering Laboratory, Key Laboratory of Oilseeds processing, Ministry of Agriculture and Rural Affairs, Hubei Key Laboratory of Lipid Chemistry and Nutrition, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, ChinaOil Crops and Lipids Process Technology National & Local Joint Engineering Laboratory, Key Laboratory of Oilseeds processing, Ministry of Agriculture and Rural Affairs, Hubei Key Laboratory of Lipid Chemistry and Nutrition, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, China; Corresponding author.Acrylamide (ACR), widely present in heat treatment of starchy foods, has attracted much attention in chronic neurotoxin. Canolol is a crucial phenolic compound and generated in rapeseed oil during microwave pressing. The present study aimed to reveal the ameliorative effects of canolol against ACR-induced toxicity in PC12 cells and the underlying mechanism. Cells pretreated with canolol inhibited ACR-exerted cytotoxicity and cell apoptosis. Canolol attenuated the increase of intracellular ROS and MDA level induced by ACR, and restored ACR-triggered depletion of glutathione content and mitochondrial membrane potential. Furthermore, canolol upregulated expressions of phosphorylated ERK1/2 protein, and downregulated phosphorylated p38 and JNK proteins after 24 h pretreatment in ACR-treated PC12 cells. Notably, ACR-stimulated autophagy activation was hindered by canolol in counteracting ACR-exerted LC-3 conversion and promoting the expression of p62 protein. Our study demonstrated that canolol prevented ACR damage involving MAPKs pathway and autophagy in PC12 cells, providing a potential application of canolol for ACR toxicity.http://www.sciencedirect.com/science/article/pii/S1756464620304813AcrylamideToxicityCanololMAPKsAutophagy |
| spellingShingle | Xiaoyang Xia Zhen Zhang Chang Zheng Qianchun Deng Mingming Zheng Ling Han Xia Xiang Ameliorative effects of canolol against acrylamide toxicity in PC12 cells through modulating MAPKs pathway and autophagy Journal of Functional Foods Acrylamide Toxicity Canolol MAPKs Autophagy |
| title | Ameliorative effects of canolol against acrylamide toxicity in PC12 cells through modulating MAPKs pathway and autophagy |
| title_full | Ameliorative effects of canolol against acrylamide toxicity in PC12 cells through modulating MAPKs pathway and autophagy |
| title_fullStr | Ameliorative effects of canolol against acrylamide toxicity in PC12 cells through modulating MAPKs pathway and autophagy |
| title_full_unstemmed | Ameliorative effects of canolol against acrylamide toxicity in PC12 cells through modulating MAPKs pathway and autophagy |
| title_short | Ameliorative effects of canolol against acrylamide toxicity in PC12 cells through modulating MAPKs pathway and autophagy |
| title_sort | ameliorative effects of canolol against acrylamide toxicity in pc12 cells through modulating mapks pathway and autophagy |
| topic | Acrylamide Toxicity Canolol MAPKs Autophagy |
| url | http://www.sciencedirect.com/science/article/pii/S1756464620304813 |
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