LncRNA SLC16A1-AS1 is Upregulated in Glioblastoma and Promotes Cancer Cell Proliferation by Regulating miR-149 Methylation
Yinbo Long, Heyang Li, Zhibin Jin, Xiang Zhang Department of Neurosurgery, Cangzhou Central Hospital, Cangzhou City, Hebei Province, 061000, People’s Republic of ChinaCorrespondence: Xiang ZhangDepartment of Neurosurgery, Cangzhou Central Hospital, Cangzhou City, Hebei Province, 061000, Pe...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Dove Medical Press
2021-02-01
|
| Series: | Cancer Management and Research |
| Subjects: | |
| Online Access: | https://www.dovepress.com/lncrna-slc16a1-as1-is-upregulated-in-glioblastoma-and-promotes-cancer--peer-reviewed-article-CMAR |
| _version_ | 1818661889450704896 |
|---|---|
| author | Long Y Li H Jin Z Zhang X |
| author_facet | Long Y Li H Jin Z Zhang X |
| author_sort | Long Y |
| collection | DOAJ |
| description | Yinbo Long, Heyang Li, Zhibin Jin, Xiang Zhang Department of Neurosurgery, Cangzhou Central Hospital, Cangzhou City, Hebei Province, 061000, People’s Republic of ChinaCorrespondence: Xiang ZhangDepartment of Neurosurgery, Cangzhou Central Hospital, Cangzhou City, Hebei Province, 061000, People’s Republic of ChinaEmail yb5052@163.comIntroduction: LncRNA SLC16A1-AS1 has been characterized as a critical player in lung cancer, while its role in glioblastoma (GBM) is unknown. By analyzing the TCGA dataset, we observed the upregulation of SLC16A1-AS1 expression in GBM. Therefore, we aimed to investigate the role of SLC16A1-AS1 in this cancer.Methods: GBM tissues and paired non-tumor tissues were collected from 62 GBM patients through biopsy. RT-qPCR was performed to determine the expression of SLC16A1-AS1 and miR-149. Linear regression was used to analyze their correlations. The relationship between SLC16A1-AS1 and miR-149 was assessed by gain and loss of function experiments. Methylation-specific PCR (MSP) and bisulfite sequencing PCR (BSP) were performed to analyze the methylation status of miR-149. Cell proliferation was evaluated by CCK-8 assay and colony formation experiments in GBM cells.Results: We found that SLC16A1-AS1 expression was upregulated in GBM tissues, and the upregulated expression of SLC16A1-AS1 predicted poor survival of GBM patients. MiR-149 was downregulated in GBM tissues and inversely correlated with the expression of SLC16A1-AS1. In GBM cells, overexpression of SLC16A1-AS1 downregulated the expression of miR-149 and increased the methylation of miR-149 gene. In cell proliferation and colony formation assay, overexpression of SLC16A1-AS1 reduced the inhibitory effects of miR-149 on GBM cell proliferation.Conclusion: SLC16A1-AS1 may promote GBM cell proliferation by regulating miR-149 methylation. SLC16A1-AS1 can be considered as a potential diagnostic marker in GBM.Keywords: GBM, SLC16A1-AS1, miR-149, methylation, proliferation |
| first_indexed | 2024-12-17T04:52:14Z |
| format | Article |
| id | doaj.art-c7164ac43529411ab3011e11d98db989 |
| institution | Directory Open Access Journal |
| issn | 1179-1322 |
| language | English |
| last_indexed | 2024-12-17T04:52:14Z |
| publishDate | 2021-02-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Cancer Management and Research |
| spelling | doaj.art-c7164ac43529411ab3011e11d98db9892022-12-21T22:02:52ZengDove Medical PressCancer Management and Research1179-13222021-02-01Volume 131215122361892LncRNA SLC16A1-AS1 is Upregulated in Glioblastoma and Promotes Cancer Cell Proliferation by Regulating miR-149 MethylationLong YLi HJin ZZhang XYinbo Long, Heyang Li, Zhibin Jin, Xiang Zhang Department of Neurosurgery, Cangzhou Central Hospital, Cangzhou City, Hebei Province, 061000, People’s Republic of ChinaCorrespondence: Xiang ZhangDepartment of Neurosurgery, Cangzhou Central Hospital, Cangzhou City, Hebei Province, 061000, People’s Republic of ChinaEmail yb5052@163.comIntroduction: LncRNA SLC16A1-AS1 has been characterized as a critical player in lung cancer, while its role in glioblastoma (GBM) is unknown. By analyzing the TCGA dataset, we observed the upregulation of SLC16A1-AS1 expression in GBM. Therefore, we aimed to investigate the role of SLC16A1-AS1 in this cancer.Methods: GBM tissues and paired non-tumor tissues were collected from 62 GBM patients through biopsy. RT-qPCR was performed to determine the expression of SLC16A1-AS1 and miR-149. Linear regression was used to analyze their correlations. The relationship between SLC16A1-AS1 and miR-149 was assessed by gain and loss of function experiments. Methylation-specific PCR (MSP) and bisulfite sequencing PCR (BSP) were performed to analyze the methylation status of miR-149. Cell proliferation was evaluated by CCK-8 assay and colony formation experiments in GBM cells.Results: We found that SLC16A1-AS1 expression was upregulated in GBM tissues, and the upregulated expression of SLC16A1-AS1 predicted poor survival of GBM patients. MiR-149 was downregulated in GBM tissues and inversely correlated with the expression of SLC16A1-AS1. In GBM cells, overexpression of SLC16A1-AS1 downregulated the expression of miR-149 and increased the methylation of miR-149 gene. In cell proliferation and colony formation assay, overexpression of SLC16A1-AS1 reduced the inhibitory effects of miR-149 on GBM cell proliferation.Conclusion: SLC16A1-AS1 may promote GBM cell proliferation by regulating miR-149 methylation. SLC16A1-AS1 can be considered as a potential diagnostic marker in GBM.Keywords: GBM, SLC16A1-AS1, miR-149, methylation, proliferationhttps://www.dovepress.com/lncrna-slc16a1-as1-is-upregulated-in-glioblastoma-and-promotes-cancer--peer-reviewed-article-CMARgbmslc16a1-as1mir-149methylationproliferation |
| spellingShingle | Long Y Li H Jin Z Zhang X LncRNA SLC16A1-AS1 is Upregulated in Glioblastoma and Promotes Cancer Cell Proliferation by Regulating miR-149 Methylation Cancer Management and Research gbm slc16a1-as1 mir-149 methylation proliferation |
| title | LncRNA SLC16A1-AS1 is Upregulated in Glioblastoma and Promotes Cancer Cell Proliferation by Regulating miR-149 Methylation |
| title_full | LncRNA SLC16A1-AS1 is Upregulated in Glioblastoma and Promotes Cancer Cell Proliferation by Regulating miR-149 Methylation |
| title_fullStr | LncRNA SLC16A1-AS1 is Upregulated in Glioblastoma and Promotes Cancer Cell Proliferation by Regulating miR-149 Methylation |
| title_full_unstemmed | LncRNA SLC16A1-AS1 is Upregulated in Glioblastoma and Promotes Cancer Cell Proliferation by Regulating miR-149 Methylation |
| title_short | LncRNA SLC16A1-AS1 is Upregulated in Glioblastoma and Promotes Cancer Cell Proliferation by Regulating miR-149 Methylation |
| title_sort | lncrna slc16a1 as1 is upregulated in glioblastoma and promotes cancer cell proliferation by regulating mir 149 methylation |
| topic | gbm slc16a1-as1 mir-149 methylation proliferation |
| url | https://www.dovepress.com/lncrna-slc16a1-as1-is-upregulated-in-glioblastoma-and-promotes-cancer--peer-reviewed-article-CMAR |
| work_keys_str_mv | AT longy lncrnaslc16a1as1isupregulatedinglioblastomaandpromotescancercellproliferationbyregulatingmir149methylation AT lih lncrnaslc16a1as1isupregulatedinglioblastomaandpromotescancercellproliferationbyregulatingmir149methylation AT jinz lncrnaslc16a1as1isupregulatedinglioblastomaandpromotescancercellproliferationbyregulatingmir149methylation AT zhangx lncrnaslc16a1as1isupregulatedinglioblastomaandpromotescancercellproliferationbyregulatingmir149methylation |