The first evidence of Acidithiobacillus albertensis in weathered ore samples from active gold mine Hodruša-Hámre (Slovakia)
Sulphur-oxidising autotrophic bacterial communities in deep biosphere from weathered ore samples from active gold mine Hodruša-Hámre, Slovakia were analysed using cultivation approach followed by DNA extraction, PCR amplification and 16S rRNA gene analyses. Indirect measurement of pH changes in cult...
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| Format: | Article |
| Language: | English |
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SciCell s.r.o.
2019-06-01
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| Series: | Nova Biotechnologica et Chimica |
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| Online Access: | http://www.degruyter.com/view/j/nbec.2019.18.issue-1/nbec-2019-0003/nbec-2019-0003.xml?format=INT |
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| author | Sedláková-Kaduková Jana Kisková Jana Maliničová Lenka Timková Ivana Jeleň Stanislav Pristaš Peter |
| author_facet | Sedláková-Kaduková Jana Kisková Jana Maliničová Lenka Timková Ivana Jeleň Stanislav Pristaš Peter |
| author_sort | Sedláková-Kaduková Jana |
| collection | DOAJ |
| description | Sulphur-oxidising autotrophic bacterial communities in deep biosphere from weathered ore samples from active gold mine Hodruša-Hámre, Slovakia were analysed using cultivation approach followed by DNA extraction, PCR amplification and 16S rRNA gene analyses. Indirect measurement of pH changes in cultivation media evidenced the presence of acidophilic bacteria with active production of acids. The decrease of pH was observed at the beginning of isolation and later pH in range of 1.5 – 2 was maintained in both, sulphuric acid and thiosulphate, media. The presence of homogenous population of gram-negative rods was proved by Gram staining. Molecular analyses have revealed that the population of sulphur-oxidising bacteria in gold mine is dominated by a single species of Aciditiobacillus genus, identified as A. albertensis, suggesting the low level of autotrophic bacteria diversity in deep deposits. For the first time this species was isolated from weathered rocks of a gold mine subsurface environment. |
| first_indexed | 2024-04-13T17:47:20Z |
| format | Article |
| id | doaj.art-c7168174c6ac498eab949ea0597727ee |
| institution | Directory Open Access Journal |
| issn | 1339-004X |
| language | English |
| last_indexed | 2024-04-13T17:47:20Z |
| publishDate | 2019-06-01 |
| publisher | SciCell s.r.o. |
| record_format | Article |
| series | Nova Biotechnologica et Chimica |
| spelling | doaj.art-c7168174c6ac498eab949ea0597727ee2022-12-22T02:36:55ZengSciCell s.r.o.Nova Biotechnologica et Chimica1339-004X2019-06-01181182410.2478/nbec-2019-0003nbec-2019-0003The first evidence of Acidithiobacillus albertensis in weathered ore samples from active gold mine Hodruša-Hámre (Slovakia)Sedláková-Kaduková Jana0Kisková Jana1Maliničová Lenka2Timková Ivana3Jeleň Stanislav4Pristaš Peter5Department of Microbiology, Institute of Biology and Ecology, Faculty of Science, Pavol Jozef Šafarik University in Košice, Šrobárova 2, SK-041 54 Košice, Slovak RepublicDepartment of Microbiology, Institute of Biology and Ecology, Faculty of Science, Pavol Jozef Šafarik University in Košice, Šrobárova 2, SK-041 54 Košice, Slovak RepublicDepartment of Microbiology, Institute of Biology and Ecology, Faculty of Science, Pavol Jozef Šafarik University in Košice, Šrobárova 2, SK-041 54 Košice, Slovak RepublicDepartment of Microbiology, Institute of Biology and Ecology, Faculty of Science, Pavol Jozef Šafarik University in Košice, Šrobárova 2, SK-041 54 Košice, Slovak RepublicEarth Science Institute, Slovak Academy of Sciences, Ďumbierska 1, SK-974 01, Banská Bystrica, Slovak RepublicDepartment of Microbiology, Institute of Biology and Ecology, Faculty of Science, Pavol Jozef Šafarik University in Košice, Šrobárova 2, SK-041 54 Košice, Slovak RepublicSulphur-oxidising autotrophic bacterial communities in deep biosphere from weathered ore samples from active gold mine Hodruša-Hámre, Slovakia were analysed using cultivation approach followed by DNA extraction, PCR amplification and 16S rRNA gene analyses. Indirect measurement of pH changes in cultivation media evidenced the presence of acidophilic bacteria with active production of acids. The decrease of pH was observed at the beginning of isolation and later pH in range of 1.5 – 2 was maintained in both, sulphuric acid and thiosulphate, media. The presence of homogenous population of gram-negative rods was proved by Gram staining. Molecular analyses have revealed that the population of sulphur-oxidising bacteria in gold mine is dominated by a single species of Aciditiobacillus genus, identified as A. albertensis, suggesting the low level of autotrophic bacteria diversity in deep deposits. For the first time this species was isolated from weathered rocks of a gold mine subsurface environment.http://www.degruyter.com/view/j/nbec.2019.18.issue-1/nbec-2019-0003/nbec-2019-0003.xml?format=INTAutotrophic bacteriaAcidithiobacillus albertensisGold mine |
| spellingShingle | Sedláková-Kaduková Jana Kisková Jana Maliničová Lenka Timková Ivana Jeleň Stanislav Pristaš Peter The first evidence of Acidithiobacillus albertensis in weathered ore samples from active gold mine Hodruša-Hámre (Slovakia) Nova Biotechnologica et Chimica Autotrophic bacteria Acidithiobacillus albertensis Gold mine |
| title | The first evidence of Acidithiobacillus albertensis in weathered ore samples from active gold mine Hodruša-Hámre (Slovakia) |
| title_full | The first evidence of Acidithiobacillus albertensis in weathered ore samples from active gold mine Hodruša-Hámre (Slovakia) |
| title_fullStr | The first evidence of Acidithiobacillus albertensis in weathered ore samples from active gold mine Hodruša-Hámre (Slovakia) |
| title_full_unstemmed | The first evidence of Acidithiobacillus albertensis in weathered ore samples from active gold mine Hodruša-Hámre (Slovakia) |
| title_short | The first evidence of Acidithiobacillus albertensis in weathered ore samples from active gold mine Hodruša-Hámre (Slovakia) |
| title_sort | first evidence of acidithiobacillus albertensis in weathered ore samples from active gold mine hodrusa hamre slovakia |
| topic | Autotrophic bacteria Acidithiobacillus albertensis Gold mine |
| url | http://www.degruyter.com/view/j/nbec.2019.18.issue-1/nbec-2019-0003/nbec-2019-0003.xml?format=INT |
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