Dynamic changes of peptidome and release of polysaccharide in sea cucumber (Apostichopus japonicus) hydrolysates depending on enzymatic hydrolysis approaches
Enzymatic hydrolysis has been widely used to produce bioactive hydrolysates from sea cucumber body wall. Here, inspired by the clarification of Apostichopus japonicus genome, we investigated the enzymatic hydrolysis of sea cucumber body wall by using the omics strategy. Shared proteins, including ma...
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| Format: | Article |
| Language: | English |
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Tsinghua University Press
2022-09-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453022000635 |
| _version_ | 1797713443766140928 |
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| author | Yanchao Wang Yan Song Yaoguang Chang Yanyan Liu Guangning Chen Changhu Xue |
| author_facet | Yanchao Wang Yan Song Yaoguang Chang Yanyan Liu Guangning Chen Changhu Xue |
| author_sort | Yanchao Wang |
| collection | DOAJ |
| description | Enzymatic hydrolysis has been widely used to produce bioactive hydrolysates from sea cucumber body wall. Here, inspired by the clarification of Apostichopus japonicus genome, we investigated the enzymatic hydrolysis of sea cucumber body wall by using the omics strategy. Shared proteins, including major yolk proteins, collagens, extracellular matrix glycoproteins and muscle proteins, were released from the body wall by different hydrolysis condition. A portfolio of 216 shared peptides were detected in the peptidome by papain with different hydrolysis time, while 32 shared peptides were detected in the peptidome by differing proteases. Unshared peptides and the relative abundance distribution profiles of shared peptides changed depending on hydrolysis approaches, indicating dynamic changes of peptidome during hydrolysis. Moreover, release of sulfated fucan and fucosylated chondroitin sulfate changed with the hydrolysis condition. The monitoring of dynamic enzymatic hydrolysis process at a molecular scale would contribute to production and quality control of sea cucumber hydrolysates. |
| first_indexed | 2024-03-12T07:36:41Z |
| format | Article |
| id | doaj.art-c719535041674d93890d2d878c66323c |
| institution | Directory Open Access Journal |
| issn | 2213-4530 |
| language | English |
| last_indexed | 2024-03-12T07:36:41Z |
| publishDate | 2022-09-01 |
| publisher | Tsinghua University Press |
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| series | Food Science and Human Wellness |
| spelling | doaj.art-c719535041674d93890d2d878c66323c2023-09-02T21:29:20ZengTsinghua University PressFood Science and Human Wellness2213-45302022-09-0111513311341Dynamic changes of peptidome and release of polysaccharide in sea cucumber (Apostichopus japonicus) hydrolysates depending on enzymatic hydrolysis approachesYanchao Wang0Yan Song1Yaoguang Chang2Yanyan Liu3Guangning Chen4Changhu Xue5College of Food Science and Engineering, Ocean University of China, Qingdao 266003, ChinaCollege of Food Science and Engineering, Ocean University of China, Qingdao 266003, ChinaCollege of Food Science and Engineering, Ocean University of China, Qingdao 266003, China; Laboratory for Marine Drugs and Bioproducts, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China; Corresponding author at: College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China.College of Food Science and Engineering, Ocean University of China, Qingdao 266003, ChinaCollege of Food Science and Engineering, Ocean University of China, Qingdao 266003, ChinaCollege of Food Science and Engineering, Ocean University of China, Qingdao 266003, China; Laboratory for Marine Drugs and Bioproducts, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, ChinaEnzymatic hydrolysis has been widely used to produce bioactive hydrolysates from sea cucumber body wall. Here, inspired by the clarification of Apostichopus japonicus genome, we investigated the enzymatic hydrolysis of sea cucumber body wall by using the omics strategy. Shared proteins, including major yolk proteins, collagens, extracellular matrix glycoproteins and muscle proteins, were released from the body wall by different hydrolysis condition. A portfolio of 216 shared peptides were detected in the peptidome by papain with different hydrolysis time, while 32 shared peptides were detected in the peptidome by differing proteases. Unshared peptides and the relative abundance distribution profiles of shared peptides changed depending on hydrolysis approaches, indicating dynamic changes of peptidome during hydrolysis. Moreover, release of sulfated fucan and fucosylated chondroitin sulfate changed with the hydrolysis condition. The monitoring of dynamic enzymatic hydrolysis process at a molecular scale would contribute to production and quality control of sea cucumber hydrolysates.http://www.sciencedirect.com/science/article/pii/S2213453022000635Sea cucumberEnzymatic hydrolysisProteinPeptidomePolysaccharide |
| spellingShingle | Yanchao Wang Yan Song Yaoguang Chang Yanyan Liu Guangning Chen Changhu Xue Dynamic changes of peptidome and release of polysaccharide in sea cucumber (Apostichopus japonicus) hydrolysates depending on enzymatic hydrolysis approaches Food Science and Human Wellness Sea cucumber Enzymatic hydrolysis Protein Peptidome Polysaccharide |
| title | Dynamic changes of peptidome and release of polysaccharide in sea cucumber (Apostichopus japonicus) hydrolysates depending on enzymatic hydrolysis approaches |
| title_full | Dynamic changes of peptidome and release of polysaccharide in sea cucumber (Apostichopus japonicus) hydrolysates depending on enzymatic hydrolysis approaches |
| title_fullStr | Dynamic changes of peptidome and release of polysaccharide in sea cucumber (Apostichopus japonicus) hydrolysates depending on enzymatic hydrolysis approaches |
| title_full_unstemmed | Dynamic changes of peptidome and release of polysaccharide in sea cucumber (Apostichopus japonicus) hydrolysates depending on enzymatic hydrolysis approaches |
| title_short | Dynamic changes of peptidome and release of polysaccharide in sea cucumber (Apostichopus japonicus) hydrolysates depending on enzymatic hydrolysis approaches |
| title_sort | dynamic changes of peptidome and release of polysaccharide in sea cucumber apostichopus japonicus hydrolysates depending on enzymatic hydrolysis approaches |
| topic | Sea cucumber Enzymatic hydrolysis Protein Peptidome Polysaccharide |
| url | http://www.sciencedirect.com/science/article/pii/S2213453022000635 |
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