The Distribution of IgT mRNA<sup>+</sup> Cells in the Gut of the Atlantic Salmon (<i>Salmo salar</i> L.)
The newly discovered IgT<sup>+</sup> B cell is thought to play a dominant role in mucosal immunity, but limited studies have examined its distribution in fish species, hindering our understanding of its function. This study investigated IgT and poly Ig receptor (pIgR) mRNA<sup>+<...
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MDPI AG
2023-10-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/13/20/3191 |
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| author | Pedro Luis Castro Fran Barac Tom Johnny Hansen Per Gunnar Fjelldal Ivar Hordvik Håvard Bjørgen Erling Olaf Koppang |
| author_facet | Pedro Luis Castro Fran Barac Tom Johnny Hansen Per Gunnar Fjelldal Ivar Hordvik Håvard Bjørgen Erling Olaf Koppang |
| author_sort | Pedro Luis Castro |
| collection | DOAJ |
| description | The newly discovered IgT<sup>+</sup> B cell is thought to play a dominant role in mucosal immunity, but limited studies have examined its distribution in fish species, hindering our understanding of its function. This study investigated IgT and poly Ig receptor (pIgR) mRNA<sup>+</sup> cell distribution in Atlantic salmon (<i>Salmo salar</i>) gut using RNAscope in situ hybridization (ISH) and assessed the effects of vaccination. The pyloric caeca, mid-intestine (first and second parts), and posterior segment in two weight stages (Group 1: avg. 153 g, Group 2: avg. 1717 g) were examined in both vaccinated and unvaccinated fish. ISH revealed more IgT mRNA<sup>+</sup> cells in the second part of the midgut compared to other intestinal segments, as well as a higher number of positive cells in Group 2 (older fish). In line with previous findings, intraperitoneal vaccination had no significant impact on the number of IgT<sup>+</sup> transcripts. IgT mRNA<sup>+</sup> cells were found mostly in the <i>lamina propria</i> and near capillaries, while pIgR was registered in both the <i>lamina propria</i> and mucosa. Interestingly, vaccinated fish presented adhesions and granulomatous tissue in the peritoneum, with both IgT and pIgR mRNA<sup>+</sup> cells. Taken together, these results suggest that the distribution of IgT mRNA<sup>+</sup> cells in the intestine of Atlantic salmon is region-specific and is not affected by intraperitoneal vaccination but varies with fish age. |
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| language | English |
| last_indexed | 2024-03-10T21:30:43Z |
| publishDate | 2023-10-01 |
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| series | Animals |
| spelling | doaj.art-c71a2677bc26455e9479f8f0e44c82122023-11-19T15:24:26ZengMDPI AGAnimals2076-26152023-10-011320319110.3390/ani13203191The Distribution of IgT mRNA<sup>+</sup> Cells in the Gut of the Atlantic Salmon (<i>Salmo salar</i> L.)Pedro Luis Castro0Fran Barac1Tom Johnny Hansen2Per Gunnar Fjelldal3Ivar Hordvik4Håvard Bjørgen5Erling Olaf Koppang6GIA-ECOAQUA, Universidad de Las Palmas de Gran Canaria, 35001 Telde, SpainUnit of Anatomy, Veterinary Faculty, Norwegian University of Life Sciences, 1433 Ås, NorwayMatre Research Station, Institute of Marine Research, 5984 Matredal, NorwayMatre Research Station, Institute of Marine Research, 5984 Matredal, NorwayInstitute of Biology, University of Bergen, 5007 Bergen, NorwayUnit of Anatomy, Veterinary Faculty, Norwegian University of Life Sciences, 1433 Ås, NorwayUnit of Anatomy, Veterinary Faculty, Norwegian University of Life Sciences, 1433 Ås, NorwayThe newly discovered IgT<sup>+</sup> B cell is thought to play a dominant role in mucosal immunity, but limited studies have examined its distribution in fish species, hindering our understanding of its function. This study investigated IgT and poly Ig receptor (pIgR) mRNA<sup>+</sup> cell distribution in Atlantic salmon (<i>Salmo salar</i>) gut using RNAscope in situ hybridization (ISH) and assessed the effects of vaccination. The pyloric caeca, mid-intestine (first and second parts), and posterior segment in two weight stages (Group 1: avg. 153 g, Group 2: avg. 1717 g) were examined in both vaccinated and unvaccinated fish. ISH revealed more IgT mRNA<sup>+</sup> cells in the second part of the midgut compared to other intestinal segments, as well as a higher number of positive cells in Group 2 (older fish). In line with previous findings, intraperitoneal vaccination had no significant impact on the number of IgT<sup>+</sup> transcripts. IgT mRNA<sup>+</sup> cells were found mostly in the <i>lamina propria</i> and near capillaries, while pIgR was registered in both the <i>lamina propria</i> and mucosa. Interestingly, vaccinated fish presented adhesions and granulomatous tissue in the peritoneum, with both IgT and pIgR mRNA<sup>+</sup> cells. Taken together, these results suggest that the distribution of IgT mRNA<sup>+</sup> cells in the intestine of Atlantic salmon is region-specific and is not affected by intraperitoneal vaccination but varies with fish age.https://www.mdpi.com/2076-2615/13/20/3191Atlantic salmongutIgTpIgRin situ hybridizationmucosal immunity |
| spellingShingle | Pedro Luis Castro Fran Barac Tom Johnny Hansen Per Gunnar Fjelldal Ivar Hordvik Håvard Bjørgen Erling Olaf Koppang The Distribution of IgT mRNA<sup>+</sup> Cells in the Gut of the Atlantic Salmon (<i>Salmo salar</i> L.) Animals Atlantic salmon gut IgT pIgR in situ hybridization mucosal immunity |
| title | The Distribution of IgT mRNA<sup>+</sup> Cells in the Gut of the Atlantic Salmon (<i>Salmo salar</i> L.) |
| title_full | The Distribution of IgT mRNA<sup>+</sup> Cells in the Gut of the Atlantic Salmon (<i>Salmo salar</i> L.) |
| title_fullStr | The Distribution of IgT mRNA<sup>+</sup> Cells in the Gut of the Atlantic Salmon (<i>Salmo salar</i> L.) |
| title_full_unstemmed | The Distribution of IgT mRNA<sup>+</sup> Cells in the Gut of the Atlantic Salmon (<i>Salmo salar</i> L.) |
| title_short | The Distribution of IgT mRNA<sup>+</sup> Cells in the Gut of the Atlantic Salmon (<i>Salmo salar</i> L.) |
| title_sort | distribution of igt mrna sup sup cells in the gut of the atlantic salmon i salmo salar i l |
| topic | Atlantic salmon gut IgT pIgR in situ hybridization mucosal immunity |
| url | https://www.mdpi.com/2076-2615/13/20/3191 |
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