Effect of Avocado Seed Ethanol Extract (Persea americana Mill) on Superoxide Dismutase (SOD1) and Histological Expression of Pancreas in Rats (Rattus norvegicus) with Diabetes Mellitus
Diabetes mellitus causes a pancreatic β-cells damage due to the increase of oxidative stress, thereby reducing Superoxide Dismutase 1 (SOD1) activity. This enzyme activity can be increased by utilizing antioxidant compounds from the avocado seed extract. This study aims to determine the increase of...
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| Format: | Article |
| Language: | English |
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University of Brawijaya
2023-08-01
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| Series: | Journal of Pure and Applied Chemistry Research |
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| Online Access: | https://jpacr.ub.ac.id/index.php/jpacr/article/view/3297/pdf |
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| author | Fauziah Fauziah Nurhaida Nurhaida Binawati Ginting Aulanni’am |
| author_facet | Fauziah Fauziah Nurhaida Nurhaida Binawati Ginting Aulanni’am |
| author_sort | Fauziah Fauziah |
| collection | DOAJ |
| description | Diabetes mellitus causes a pancreatic β-cells damage due to the increase of oxidative stress, thereby reducing Superoxide Dismutase 1 (SOD1) activity. This enzyme activity can be increased by utilizing antioxidant compounds from the avocado seed extract. This study aims to determine the increase of SOD1 expression and the repair of pancreatic β-cells tretaed with the ethanol extract of avocado seeds (Persea americana Mill). It is also aimed to observe the expression of pancreatic SOD1 and its histopathology change. The expression was measured by using immunohistochemical. This research used 20 Wistar rats aged of 2-3 moths, weighing of 150-200 grams. The diabetic rats were induced by streptozotocin (STZ) with dose of 30 mg/kg BW intraperitoneally. The rats were divided into 5 groups, namely: group 1 is healthy rats, group 2 is diabetic rats, while the group 3, 4 and 5 are therapeutic groups with 300, 350 and 400 mg/kg BW doses of ethanol extract for 14 days treatment. The results showed the ethanolic extract of avocado seeds has a potential as an antidiabetic agents based on the increase of pancreatic SOD1 (p<0.05) significantly, increase of SOD1 expression 7.6 ± 1.34, 11.6 ± 1.94 and 13.0 ± 2.82 in three different doses therapy (300, 350 and 400 mg/kg BW), and repairing structure of β-pancreatic cells
on the therapeutic groups. |
| first_indexed | 2024-03-11T15:07:09Z |
| format | Article |
| id | doaj.art-c71bf8fcb1cd41ab884e0b2a1b473d48 |
| institution | Directory Open Access Journal |
| issn | 2302-4690 2541-0733 |
| language | English |
| last_indexed | 2024-03-11T15:07:09Z |
| publishDate | 2023-08-01 |
| publisher | University of Brawijaya |
| record_format | Article |
| series | Journal of Pure and Applied Chemistry Research |
| spelling | doaj.art-c71bf8fcb1cd41ab884e0b2a1b473d482023-10-30T04:56:47ZengUniversity of BrawijayaJournal of Pure and Applied Chemistry Research2302-46902541-07332023-08-01122 8086Effect of Avocado Seed Ethanol Extract (Persea americana Mill) on Superoxide Dismutase (SOD1) and Histological Expression of Pancreas in Rats (Rattus norvegicus) with Diabetes MellitusFauziah Fauziah0Nurhaida Nurhaida1Binawati Ginting2Aulanni’am3Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Syiah Kuala Banda Aceh, IndonesiaDepartment of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Syiah Kuala Banda Aceh, IndonesiaDepartment of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Syiah Kuala Banda Aceh, IndonesiaBiochemistry Laboratory, Faculty of Mathematics and Natural Sciences, Universitas Brawijaya, Malang, Indonesia; Faculty of Veterinary Medicine, Universitas Brawijaya, Malang, IndonesiaDiabetes mellitus causes a pancreatic β-cells damage due to the increase of oxidative stress, thereby reducing Superoxide Dismutase 1 (SOD1) activity. This enzyme activity can be increased by utilizing antioxidant compounds from the avocado seed extract. This study aims to determine the increase of SOD1 expression and the repair of pancreatic β-cells tretaed with the ethanol extract of avocado seeds (Persea americana Mill). It is also aimed to observe the expression of pancreatic SOD1 and its histopathology change. The expression was measured by using immunohistochemical. This research used 20 Wistar rats aged of 2-3 moths, weighing of 150-200 grams. The diabetic rats were induced by streptozotocin (STZ) with dose of 30 mg/kg BW intraperitoneally. The rats were divided into 5 groups, namely: group 1 is healthy rats, group 2 is diabetic rats, while the group 3, 4 and 5 are therapeutic groups with 300, 350 and 400 mg/kg BW doses of ethanol extract for 14 days treatment. The results showed the ethanolic extract of avocado seeds has a potential as an antidiabetic agents based on the increase of pancreatic SOD1 (p<0.05) significantly, increase of SOD1 expression 7.6 ± 1.34, 11.6 ± 1.94 and 13.0 ± 2.82 in three different doses therapy (300, 350 and 400 mg/kg BW), and repairing structure of β-pancreatic cells on the therapeutic groups.https://jpacr.ub.ac.id/index.php/jpacr/article/view/3297/pdfavocado seed ethanol extractdiabetes mellitushematoxylin-eosinsuperoxide dismutase |
| spellingShingle | Fauziah Fauziah Nurhaida Nurhaida Binawati Ginting Aulanni’am Effect of Avocado Seed Ethanol Extract (Persea americana Mill) on Superoxide Dismutase (SOD1) and Histological Expression of Pancreas in Rats (Rattus norvegicus) with Diabetes Mellitus Journal of Pure and Applied Chemistry Research avocado seed ethanol extract diabetes mellitus hematoxylin-eosin superoxide dismutase |
| title | Effect of Avocado Seed Ethanol Extract (Persea americana Mill) on Superoxide Dismutase (SOD1) and Histological Expression of Pancreas in Rats (Rattus norvegicus) with Diabetes Mellitus |
| title_full | Effect of Avocado Seed Ethanol Extract (Persea americana Mill) on Superoxide Dismutase (SOD1) and Histological Expression of Pancreas in Rats (Rattus norvegicus) with Diabetes Mellitus |
| title_fullStr | Effect of Avocado Seed Ethanol Extract (Persea americana Mill) on Superoxide Dismutase (SOD1) and Histological Expression of Pancreas in Rats (Rattus norvegicus) with Diabetes Mellitus |
| title_full_unstemmed | Effect of Avocado Seed Ethanol Extract (Persea americana Mill) on Superoxide Dismutase (SOD1) and Histological Expression of Pancreas in Rats (Rattus norvegicus) with Diabetes Mellitus |
| title_short | Effect of Avocado Seed Ethanol Extract (Persea americana Mill) on Superoxide Dismutase (SOD1) and Histological Expression of Pancreas in Rats (Rattus norvegicus) with Diabetes Mellitus |
| title_sort | effect of avocado seed ethanol extract persea americana mill on superoxide dismutase sod1 and histological expression of pancreas in rats rattus norvegicus with diabetes mellitus |
| topic | avocado seed ethanol extract diabetes mellitus hematoxylin-eosin superoxide dismutase |
| url | https://jpacr.ub.ac.id/index.php/jpacr/article/view/3297/pdf |
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