Deployment of a Novel Organic Acid Compound Disinfectant against Common Foodborne Pathogens
Background: The disinfection process represents an important activity closely linked to the removal of micro-organisms in common processing systems. Traditional disinfectants are often not sufficient to avoid the spread of food pathogens; therefore, innovative strategies for decontamination are cruc...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-12-01
|
| Series: | Toxics |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2305-6304/10/12/768 |
| _version_ | 1797455126664839168 |
|---|---|
| author | Veronica Folliero Maria Ricciardi Federica Dell’Annunziata Concetta Pironti Massimiliano Galdiero Gianluigi Franci Oriana Motta Antonio Proto |
| author_facet | Veronica Folliero Maria Ricciardi Federica Dell’Annunziata Concetta Pironti Massimiliano Galdiero Gianluigi Franci Oriana Motta Antonio Proto |
| author_sort | Veronica Folliero |
| collection | DOAJ |
| description | Background: The disinfection process represents an important activity closely linked to the removal of micro-organisms in common processing systems. Traditional disinfectants are often not sufficient to avoid the spread of food pathogens; therefore, innovative strategies for decontamination are crucial to countering microbial transmission. This study aims to assess the antimicrobial efficiency of tetrapotassium iminodisuccinic acid salt (IDSK) against the most common pathogens present on surfaces, especially in food-borne environments. Methods: IDSK was synthesized from maleic anhydride and characterized through nuclear magnetic resonance (NMR) spectroscopy (both <sup>1</sup>H-NMR and <sup>13</sup>C-NMR), thermogravimetric analysis (TGA) and Fourier Transform Infrared (FTIR) spectroscopy. The antibacterial activity was performed via the broth microdilution method and time-killing assays against <i>Escherichia coli</i>, <i>Staphylococcus aureus</i>, <i>Salmonella enterica</i>, <i>Enterococcus faecalis</i> and <i>Pseudomonas aeruginosa</i> (IDSK concentration range: 0.5–0.002 M). The biofilm biomass eradicating activity was assessed via a crystal violet (CV) assay. Results: The minimum inhibitory concentration (MIC) of IDSK was 0.25 M for all tested strains, exerting bacteriostatic action. IDSK also reduced biofilm biomass in a dose-dependent manner, reaching rates of about 50% eradication at a dose of 0.25 M. The advantages of using this innovative compound are not limited to disinfecting efficiency but also include its high biodegradability and its sustainable synthesis. Conclusions: IDSK could represent an innovative and advantageous disinfectant for food processing and workers’ activities, leading to a better quality of food and safer working conditions for the operators. |
| first_indexed | 2024-03-09T15:47:02Z |
| format | Article |
| id | doaj.art-c7217d84d6754a81b2f95db27dea1bc7 |
| institution | Directory Open Access Journal |
| issn | 2305-6304 |
| language | English |
| last_indexed | 2024-03-09T15:47:02Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxics |
| spelling | doaj.art-c7217d84d6754a81b2f95db27dea1bc72023-11-24T18:25:34ZengMDPI AGToxics2305-63042022-12-01101276810.3390/toxics10120768Deployment of a Novel Organic Acid Compound Disinfectant against Common Foodborne PathogensVeronica Folliero0Maria Ricciardi1Federica Dell’Annunziata2Concetta Pironti3Massimiliano Galdiero4Gianluigi Franci5Oriana Motta6Antonio Proto7Department of Experimental Medicine, University of Campania “Luigi Vanvitelli”, Via S. Maria di Costantinopoli, 16, 80138 Naples, ItalyDepartment of Chemistry and Biology, University of Salerno, Via Giovanni Paolo II, 132-84084 Fisciano, ItalyDepartment of Experimental Medicine, University of Campania “Luigi Vanvitelli”, Via S. Maria di Costantinopoli, 16, 80138 Naples, ItalyDepartment of Medicine Surgery and Dentistry, University of Salerno, Via S. Allende, 84081 Baronissi, ItalyDepartment of Experimental Medicine, University of Campania “Luigi Vanvitelli”, Via S. Maria di Costantinopoli, 16, 80138 Naples, ItalyDepartment of Medicine Surgery and Dentistry, University of Salerno, Via S. Allende, 84081 Baronissi, ItalyDepartment of Medicine Surgery and Dentistry, University of Salerno, Via S. Allende, 84081 Baronissi, ItalyDepartment of Chemistry and Biology, University of Salerno, Via Giovanni Paolo II, 132-84084 Fisciano, ItalyBackground: The disinfection process represents an important activity closely linked to the removal of micro-organisms in common processing systems. Traditional disinfectants are often not sufficient to avoid the spread of food pathogens; therefore, innovative strategies for decontamination are crucial to countering microbial transmission. This study aims to assess the antimicrobial efficiency of tetrapotassium iminodisuccinic acid salt (IDSK) against the most common pathogens present on surfaces, especially in food-borne environments. Methods: IDSK was synthesized from maleic anhydride and characterized through nuclear magnetic resonance (NMR) spectroscopy (both <sup>1</sup>H-NMR and <sup>13</sup>C-NMR), thermogravimetric analysis (TGA) and Fourier Transform Infrared (FTIR) spectroscopy. The antibacterial activity was performed via the broth microdilution method and time-killing assays against <i>Escherichia coli</i>, <i>Staphylococcus aureus</i>, <i>Salmonella enterica</i>, <i>Enterococcus faecalis</i> and <i>Pseudomonas aeruginosa</i> (IDSK concentration range: 0.5–0.002 M). The biofilm biomass eradicating activity was assessed via a crystal violet (CV) assay. Results: The minimum inhibitory concentration (MIC) of IDSK was 0.25 M for all tested strains, exerting bacteriostatic action. IDSK also reduced biofilm biomass in a dose-dependent manner, reaching rates of about 50% eradication at a dose of 0.25 M. The advantages of using this innovative compound are not limited to disinfecting efficiency but also include its high biodegradability and its sustainable synthesis. Conclusions: IDSK could represent an innovative and advantageous disinfectant for food processing and workers’ activities, leading to a better quality of food and safer working conditions for the operators.https://www.mdpi.com/2305-6304/10/12/768iminodisuccinic acid potassium saltchelating activitybiofilm removalfood-borne pathogensworkers’ safety |
| spellingShingle | Veronica Folliero Maria Ricciardi Federica Dell’Annunziata Concetta Pironti Massimiliano Galdiero Gianluigi Franci Oriana Motta Antonio Proto Deployment of a Novel Organic Acid Compound Disinfectant against Common Foodborne Pathogens Toxics iminodisuccinic acid potassium salt chelating activity biofilm removal food-borne pathogens workers’ safety |
| title | Deployment of a Novel Organic Acid Compound Disinfectant against Common Foodborne Pathogens |
| title_full | Deployment of a Novel Organic Acid Compound Disinfectant against Common Foodborne Pathogens |
| title_fullStr | Deployment of a Novel Organic Acid Compound Disinfectant against Common Foodborne Pathogens |
| title_full_unstemmed | Deployment of a Novel Organic Acid Compound Disinfectant against Common Foodborne Pathogens |
| title_short | Deployment of a Novel Organic Acid Compound Disinfectant against Common Foodborne Pathogens |
| title_sort | deployment of a novel organic acid compound disinfectant against common foodborne pathogens |
| topic | iminodisuccinic acid potassium salt chelating activity biofilm removal food-borne pathogens workers’ safety |
| url | https://www.mdpi.com/2305-6304/10/12/768 |
| work_keys_str_mv | AT veronicafolliero deploymentofanovelorganicacidcompounddisinfectantagainstcommonfoodbornepathogens AT mariaricciardi deploymentofanovelorganicacidcompounddisinfectantagainstcommonfoodbornepathogens AT federicadellannunziata deploymentofanovelorganicacidcompounddisinfectantagainstcommonfoodbornepathogens AT concettapironti deploymentofanovelorganicacidcompounddisinfectantagainstcommonfoodbornepathogens AT massimilianogaldiero deploymentofanovelorganicacidcompounddisinfectantagainstcommonfoodbornepathogens AT gianluigifranci deploymentofanovelorganicacidcompounddisinfectantagainstcommonfoodbornepathogens AT orianamotta deploymentofanovelorganicacidcompounddisinfectantagainstcommonfoodbornepathogens AT antonioproto deploymentofanovelorganicacidcompounddisinfectantagainstcommonfoodbornepathogens |