Targeted Imaging of Endometriosis and Image-Guided Resection of Lesions Using Gonadotropin-Releasing Hormone Analogue-Modified Indocyanine Green
Objective. In this study, we utilized gonadotropin-releasing hormone analogue-modified indocyanine green (GnRHa-ICG) to improve the accuracy of intraoperative recognition and resection of endometriotic lesions. Methods. Gonadotropin-releasing hormone receptor (GnRHR) expression was detected in endom...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
SAGE Publications
2023-01-01
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| Series: | Molecular Imaging |
| Online Access: | http://dx.doi.org/10.1155/2023/6674054 |
| _version_ | 1797281285125701632 |
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| author | Jing Peng Qiyu Liu Tao Pu Mingxing Zhang Meng Zhang Ming Du Guiling Li Xiaoyan Zhang Congjian Xu |
| author_facet | Jing Peng Qiyu Liu Tao Pu Mingxing Zhang Meng Zhang Ming Du Guiling Li Xiaoyan Zhang Congjian Xu |
| author_sort | Jing Peng |
| collection | DOAJ |
| description | Objective. In this study, we utilized gonadotropin-releasing hormone analogue-modified indocyanine green (GnRHa-ICG) to improve the accuracy of intraoperative recognition and resection of endometriotic lesions. Methods. Gonadotropin-releasing hormone receptor (GnRHR) expression was detected in endometriosis tissues and cell lines via immunohistochemistry and western blotting. The in vitro binding capacities of GnRHa, GnRHa-ICG, and ICG were determined using fluorescence microscopy and flow cytometry. In vivo imaging was performed in mouse models of endometriosis using a near-infrared fluorescence (NIRF) imaging system and fluorescence navigation system. The ex vivo binding capacity was determined using confocal fluorescence microscopy. Results. GnRHa-ICG exhibited a significantly stronger binding capacity to endometriotic cells and tissues than ICG. In mice with endometriosis, GnRHa-ICG specifically imaged endometriotic tissues (EMTs) after intraperitoneal administration, whereas ICG exhibited signals in the intestine. GnRHa-ICG showed the highest fluorescence signals in the EMTs at 2 h and a good signal-to-noise ratio at 48 h postadministration. Compared with traditional surgery under white light, targeted NIRF imaging-guided surgery completely resected endometriotic lesions with a sensitivity of 97.3% and specificity of 77.8%. No obvious toxicity was observed in routine blood tests, serum biochemicals, or histopathology in mice. Conclusions. GnRHa-ICG specifically recognized and localized endometriotic lesions and guided complete resection of lesions with high accuracy. |
| first_indexed | 2024-03-07T16:55:18Z |
| format | Article |
| id | doaj.art-c7289c43fce849e4bacafaae8a65c0d7 |
| institution | Directory Open Access Journal |
| issn | 1536-0121 |
| language | English |
| last_indexed | 2024-03-07T16:55:18Z |
| publishDate | 2023-01-01 |
| publisher | SAGE Publications |
| record_format | Article |
| series | Molecular Imaging |
| spelling | doaj.art-c7289c43fce849e4bacafaae8a65c0d72024-03-03T04:26:55ZengSAGE PublicationsMolecular Imaging1536-01212023-01-01202310.1155/2023/6674054Targeted Imaging of Endometriosis and Image-Guided Resection of Lesions Using Gonadotropin-Releasing Hormone Analogue-Modified Indocyanine GreenJing Peng0Qiyu Liu1Tao Pu2Mingxing Zhang3Meng Zhang4Ming Du5Guiling Li6Xiaoyan Zhang7Congjian Xu8Obstetrics and Gynecology HospitalObstetrics and Gynecology HospitalObstetrics and Gynecology HospitalObstetrics and Gynecology HospitalObstetrics and Gynecology HospitalObstetrics and Gynecology HospitalObstetrics and Gynecology HospitalObstetrics and Gynecology HospitalObstetrics and Gynecology HospitalObjective. In this study, we utilized gonadotropin-releasing hormone analogue-modified indocyanine green (GnRHa-ICG) to improve the accuracy of intraoperative recognition and resection of endometriotic lesions. Methods. Gonadotropin-releasing hormone receptor (GnRHR) expression was detected in endometriosis tissues and cell lines via immunohistochemistry and western blotting. The in vitro binding capacities of GnRHa, GnRHa-ICG, and ICG were determined using fluorescence microscopy and flow cytometry. In vivo imaging was performed in mouse models of endometriosis using a near-infrared fluorescence (NIRF) imaging system and fluorescence navigation system. The ex vivo binding capacity was determined using confocal fluorescence microscopy. Results. GnRHa-ICG exhibited a significantly stronger binding capacity to endometriotic cells and tissues than ICG. In mice with endometriosis, GnRHa-ICG specifically imaged endometriotic tissues (EMTs) after intraperitoneal administration, whereas ICG exhibited signals in the intestine. GnRHa-ICG showed the highest fluorescence signals in the EMTs at 2 h and a good signal-to-noise ratio at 48 h postadministration. Compared with traditional surgery under white light, targeted NIRF imaging-guided surgery completely resected endometriotic lesions with a sensitivity of 97.3% and specificity of 77.8%. No obvious toxicity was observed in routine blood tests, serum biochemicals, or histopathology in mice. Conclusions. GnRHa-ICG specifically recognized and localized endometriotic lesions and guided complete resection of lesions with high accuracy.http://dx.doi.org/10.1155/2023/6674054 |
| spellingShingle | Jing Peng Qiyu Liu Tao Pu Mingxing Zhang Meng Zhang Ming Du Guiling Li Xiaoyan Zhang Congjian Xu Targeted Imaging of Endometriosis and Image-Guided Resection of Lesions Using Gonadotropin-Releasing Hormone Analogue-Modified Indocyanine Green Molecular Imaging |
| title | Targeted Imaging of Endometriosis and Image-Guided Resection of Lesions Using Gonadotropin-Releasing Hormone Analogue-Modified Indocyanine Green |
| title_full | Targeted Imaging of Endometriosis and Image-Guided Resection of Lesions Using Gonadotropin-Releasing Hormone Analogue-Modified Indocyanine Green |
| title_fullStr | Targeted Imaging of Endometriosis and Image-Guided Resection of Lesions Using Gonadotropin-Releasing Hormone Analogue-Modified Indocyanine Green |
| title_full_unstemmed | Targeted Imaging of Endometriosis and Image-Guided Resection of Lesions Using Gonadotropin-Releasing Hormone Analogue-Modified Indocyanine Green |
| title_short | Targeted Imaging of Endometriosis and Image-Guided Resection of Lesions Using Gonadotropin-Releasing Hormone Analogue-Modified Indocyanine Green |
| title_sort | targeted imaging of endometriosis and image guided resection of lesions using gonadotropin releasing hormone analogue modified indocyanine green |
| url | http://dx.doi.org/10.1155/2023/6674054 |
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