Cardioprotection via Metabolism for Rat Heart Preservation Using the High-Pressure Gaseous Mixture of Carbon Monoxide and Oxygen
The high-pressure gas (HPG) method with carbon monoxide (CO) and oxygen (O<sub>2</sub>) mixture maintains the preserved rat heart function. The metabolites of rat hearts preserved using the HPG method (HPG group) and cold storage (CS) method (CS group) by immersion in a stock solution fo...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-11-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/21/22/8858 |
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| author | Chiharu Suzuki Naoyuki Hatayama Tadashi Ogawa Eri Nanizawa Shun Otsuka Koichiro Hata Hiroshi Seno Munekazu Naito Shuichi Hirai |
| author_facet | Chiharu Suzuki Naoyuki Hatayama Tadashi Ogawa Eri Nanizawa Shun Otsuka Koichiro Hata Hiroshi Seno Munekazu Naito Shuichi Hirai |
| author_sort | Chiharu Suzuki |
| collection | DOAJ |
| description | The high-pressure gas (HPG) method with carbon monoxide (CO) and oxygen (O<sub>2</sub>) mixture maintains the preserved rat heart function. The metabolites of rat hearts preserved using the HPG method (HPG group) and cold storage (CS) method (CS group) by immersion in a stock solution for 24 h were assessed to confirm CO and O<sub>2</sub> effects. Lactic acid was significantly lower and citric acid was significantly higher in the HPG group than in the CS group. Moreover, adenosine triphosphate (ATP) levels as well as some pentose phosphate pathway (PPP) metabolites and reduced nicotinamide adenine dinucleotide phosphate (NADPH) were significantly higher in the HPG group than in the CS group. Additionally, reduced glutathione (GSH), which protects cells from oxidative stress, was also significantly higher in the HPG group than in the CS group. These results indicated that each gas, CO and O<sub>2</sub>, induced the shift from anaerobic to aerobic metabolism, maintaining the energy of ischemic preserved organs, shifting the glucose utilization from glycolysis toward PPP, and reducing oxidative stress. Both CO and O<sub>2</sub> in the HPG method have important effects on the ATP supply and decrease oxidative stress for preventing ischemic injury. The HPG method may be useful for clinical application. |
| first_indexed | 2024-03-10T14:39:21Z |
| format | Article |
| id | doaj.art-c728cfbbe06449b68d9281ba952b8ebb |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T14:39:21Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-c728cfbbe06449b68d9281ba952b8ebb2023-11-20T21:59:03ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-11-012122885810.3390/ijms21228858Cardioprotection via Metabolism for Rat Heart Preservation Using the High-Pressure Gaseous Mixture of Carbon Monoxide and OxygenChiharu Suzuki0Naoyuki Hatayama1Tadashi Ogawa2Eri Nanizawa3Shun Otsuka4Koichiro Hata5Hiroshi Seno6Munekazu Naito7Shuichi Hirai8Department of Anatomy, Aichi Medical University, Aichi 480-1195, JapanDepartment of Anatomy, Aichi Medical University, Aichi 480-1195, JapanDepartment of Legal Medicine, Aichi Medical University, Aichi 480-1195, JapanDepartment of Anatomy, Aichi Medical University, Aichi 480-1195, JapanDepartment of Anatomy, Aichi Medical University, Aichi 480-1195, JapanDepartment of Surgery, Division of Hepato-Biliary-Pancreatic and Transplant Surgery, Graduate School of Medicine, Kyoto University, Kyoto 606-8507, JapanDepartment of Legal Medicine, Aichi Medical University, Aichi 480-1195, JapanDepartment of Anatomy, Aichi Medical University, Aichi 480-1195, JapanDepartment of Anatomy, Aichi Medical University, Aichi 480-1195, JapanThe high-pressure gas (HPG) method with carbon monoxide (CO) and oxygen (O<sub>2</sub>) mixture maintains the preserved rat heart function. The metabolites of rat hearts preserved using the HPG method (HPG group) and cold storage (CS) method (CS group) by immersion in a stock solution for 24 h were assessed to confirm CO and O<sub>2</sub> effects. Lactic acid was significantly lower and citric acid was significantly higher in the HPG group than in the CS group. Moreover, adenosine triphosphate (ATP) levels as well as some pentose phosphate pathway (PPP) metabolites and reduced nicotinamide adenine dinucleotide phosphate (NADPH) were significantly higher in the HPG group than in the CS group. Additionally, reduced glutathione (GSH), which protects cells from oxidative stress, was also significantly higher in the HPG group than in the CS group. These results indicated that each gas, CO and O<sub>2</sub>, induced the shift from anaerobic to aerobic metabolism, maintaining the energy of ischemic preserved organs, shifting the glucose utilization from glycolysis toward PPP, and reducing oxidative stress. Both CO and O<sub>2</sub> in the HPG method have important effects on the ATP supply and decrease oxidative stress for preventing ischemic injury. The HPG method may be useful for clinical application.https://www.mdpi.com/1422-0067/21/22/8858cardioprotectionischemic injurymetabolomicscarbon monoxideoxygenhigh-pressure gas |
| spellingShingle | Chiharu Suzuki Naoyuki Hatayama Tadashi Ogawa Eri Nanizawa Shun Otsuka Koichiro Hata Hiroshi Seno Munekazu Naito Shuichi Hirai Cardioprotection via Metabolism for Rat Heart Preservation Using the High-Pressure Gaseous Mixture of Carbon Monoxide and Oxygen International Journal of Molecular Sciences cardioprotection ischemic injury metabolomics carbon monoxide oxygen high-pressure gas |
| title | Cardioprotection via Metabolism for Rat Heart Preservation Using the High-Pressure Gaseous Mixture of Carbon Monoxide and Oxygen |
| title_full | Cardioprotection via Metabolism for Rat Heart Preservation Using the High-Pressure Gaseous Mixture of Carbon Monoxide and Oxygen |
| title_fullStr | Cardioprotection via Metabolism for Rat Heart Preservation Using the High-Pressure Gaseous Mixture of Carbon Monoxide and Oxygen |
| title_full_unstemmed | Cardioprotection via Metabolism for Rat Heart Preservation Using the High-Pressure Gaseous Mixture of Carbon Monoxide and Oxygen |
| title_short | Cardioprotection via Metabolism for Rat Heart Preservation Using the High-Pressure Gaseous Mixture of Carbon Monoxide and Oxygen |
| title_sort | cardioprotection via metabolism for rat heart preservation using the high pressure gaseous mixture of carbon monoxide and oxygen |
| topic | cardioprotection ischemic injury metabolomics carbon monoxide oxygen high-pressure gas |
| url | https://www.mdpi.com/1422-0067/21/22/8858 |
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