Efficient Scavenging of TEMPOL Radical by Ascorbic Acid in Solution and Related Prolongation of <sup>13</sup>C and <sup>1</sup>H Nuclear Spin Relaxation Times of the Solute
Dynamic nuclear polarization for nuclear magnetic resonance (NMR) spectroscopy and imaging uses free radicals to strongly enhance the NMR signal of a compound under investigation. At the same time, the radicals shorten significantly its nuclear spin relaxation times which reduces the time window ava...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-02-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/29/3/738 |
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| author | Václav Římal Eleonora I. Bunyatova Helena Štěpánková |
| author_facet | Václav Římal Eleonora I. Bunyatova Helena Štěpánková |
| author_sort | Václav Římal |
| collection | DOAJ |
| description | Dynamic nuclear polarization for nuclear magnetic resonance (NMR) spectroscopy and imaging uses free radicals to strongly enhance the NMR signal of a compound under investigation. At the same time, the radicals shorten significantly its nuclear spin relaxation times which reduces the time window available for the experiments. Radical scavenging can overcome this drawback. Our work presents a detailed study of the reduction of the TEMPOL radical by ascorbic acid in solution by high-resolution NMR. Carbon-13 and hydrogen-1 nuclear spin relaxations are confirmed to be restored to their values without TEMPOL. Reaction mechanism, kinetics, and the influence of pD and viscosity are thoroughly discussed. The detailed investigation conducted in this work should help with choosing suitable concentrations in the samples for dynamic nuclear polarization and optimizing the measurement protocols. |
| first_indexed | 2024-03-08T03:52:07Z |
| format | Article |
| id | doaj.art-c729658634794638a4784c40bb1713aa |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-08T03:52:07Z |
| publishDate | 2024-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-c729658634794638a4784c40bb1713aa2024-02-09T15:19:15ZengMDPI AGMolecules1420-30492024-02-0129373810.3390/molecules29030738Efficient Scavenging of TEMPOL Radical by Ascorbic Acid in Solution and Related Prolongation of <sup>13</sup>C and <sup>1</sup>H Nuclear Spin Relaxation Times of the SoluteVáclav Římal0Eleonora I. Bunyatova1Helena Štěpánková2Faculty of Mathematics and Physics, Charles University, V Holešovičkách 2, 18000 Prague 8, Czech RepublicJoint Institute for Nuclear Research, 141980 Dubna, RussiaFaculty of Mathematics and Physics, Charles University, V Holešovičkách 2, 18000 Prague 8, Czech RepublicDynamic nuclear polarization for nuclear magnetic resonance (NMR) spectroscopy and imaging uses free radicals to strongly enhance the NMR signal of a compound under investigation. At the same time, the radicals shorten significantly its nuclear spin relaxation times which reduces the time window available for the experiments. Radical scavenging can overcome this drawback. Our work presents a detailed study of the reduction of the TEMPOL radical by ascorbic acid in solution by high-resolution NMR. Carbon-13 and hydrogen-1 nuclear spin relaxations are confirmed to be restored to their values without TEMPOL. Reaction mechanism, kinetics, and the influence of pD and viscosity are thoroughly discussed. The detailed investigation conducted in this work should help with choosing suitable concentrations in the samples for dynamic nuclear polarization and optimizing the measurement protocols.https://www.mdpi.com/1420-3049/29/3/738TEMPOLradical scavengingNMRnuclear spin relaxation |
| spellingShingle | Václav Římal Eleonora I. Bunyatova Helena Štěpánková Efficient Scavenging of TEMPOL Radical by Ascorbic Acid in Solution and Related Prolongation of <sup>13</sup>C and <sup>1</sup>H Nuclear Spin Relaxation Times of the Solute Molecules TEMPOL radical scavenging NMR nuclear spin relaxation |
| title | Efficient Scavenging of TEMPOL Radical by Ascorbic Acid in Solution and Related Prolongation of <sup>13</sup>C and <sup>1</sup>H Nuclear Spin Relaxation Times of the Solute |
| title_full | Efficient Scavenging of TEMPOL Radical by Ascorbic Acid in Solution and Related Prolongation of <sup>13</sup>C and <sup>1</sup>H Nuclear Spin Relaxation Times of the Solute |
| title_fullStr | Efficient Scavenging of TEMPOL Radical by Ascorbic Acid in Solution and Related Prolongation of <sup>13</sup>C and <sup>1</sup>H Nuclear Spin Relaxation Times of the Solute |
| title_full_unstemmed | Efficient Scavenging of TEMPOL Radical by Ascorbic Acid in Solution and Related Prolongation of <sup>13</sup>C and <sup>1</sup>H Nuclear Spin Relaxation Times of the Solute |
| title_short | Efficient Scavenging of TEMPOL Radical by Ascorbic Acid in Solution and Related Prolongation of <sup>13</sup>C and <sup>1</sup>H Nuclear Spin Relaxation Times of the Solute |
| title_sort | efficient scavenging of tempol radical by ascorbic acid in solution and related prolongation of sup 13 sup c and sup 1 sup h nuclear spin relaxation times of the solute |
| topic | TEMPOL radical scavenging NMR nuclear spin relaxation |
| url | https://www.mdpi.com/1420-3049/29/3/738 |
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