Mitotic and Proliferative Indices in WHO Grade III Meningioma
Meningiomas with inherently high mitotic indices and poor prognosis, such as WHO grade III meningiomas, have not been investigated separately to establish interchangeability between conventional mitotic index counted on H&E stained slides (MI) and mitotic index counted on phosphohistone-H3 stain...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-11-01
|
| Series: | Cancers |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2072-6694/12/11/3351 |
| _version_ | 1797548142210580480 |
|---|---|
| author | Andrea Daniela Maier Christian Beltoft Brøchner Jiri Bartek Jr. Frank Eriksson Heidi Ugleholdt Helle Broholm Tiit Mathiesen |
| author_facet | Andrea Daniela Maier Christian Beltoft Brøchner Jiri Bartek Jr. Frank Eriksson Heidi Ugleholdt Helle Broholm Tiit Mathiesen |
| author_sort | Andrea Daniela Maier |
| collection | DOAJ |
| description | Meningiomas with inherently high mitotic indices and poor prognosis, such as WHO grade III meningiomas, have not been investigated separately to establish interchangeability between conventional mitotic index counted on H&E stained slides (MI) and mitotic index counted on phosphohistone-H3 stained slides (PHH3 MI). This study investigates the agreement of MI and PHH3 MI and to analyze the association of progression-free survival (PFS) and MI, PHH3 MI, and the proliferative index (PI, Ki-67) in WHO grade III meningioma. Tumor specimens from 24 consecutive patients were analyzed for expression of Ki-67, PHH3 MI, and MI. Quantification was performed independently by two observers who made replicate counts in hot spots and overall tumor staining. Repeatability in replicate counts from MI and PHH3 MI was low in both observers. Consequently, we could not report the agreement. MI, PHH3 MI and hot spot counts of Ki-67 were associated with PFS (MI hot spot HR = 1.61, 95% CI 1.12–2.31, <i>p</i> = 0.010; PHH3 MI hot spot HR = 1.59, 95% CI 1.15–2.21, <i>p</i> = 0.006; Ki-67 hot spot HR = 1.06, 95% CI 1.02–1.11. <i>p</i> = 0.004). We found markedly low repeatability of manually counted MI and PHH3 MI in WHO grade III meningioma, and we could not conclude that the two methods agreed. Subsequently, quantification with better repeatability should be sought. All three biomarkers were associated with PFS. |
| first_indexed | 2024-03-10T14:54:16Z |
| format | Article |
| id | doaj.art-c72994f972624fca95f0d19e9c96bea3 |
| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-10T14:54:16Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-c72994f972624fca95f0d19e9c96bea32023-11-20T20:43:58ZengMDPI AGCancers2072-66942020-11-011211335110.3390/cancers12113351Mitotic and Proliferative Indices in WHO Grade III MeningiomaAndrea Daniela Maier0Christian Beltoft Brøchner1Jiri Bartek Jr.2Frank Eriksson3Heidi Ugleholdt4Helle Broholm5Tiit Mathiesen6Department of Neurosurgery, Copenhagen University Hospital, Rigshospitalet, Inge Lehmanns Vej 6, 2100 Copenhagen, DenmarkPathology Department, Copenhagen University Hospital, Rigshospitalet, Inge Lehmanns Vej 7, 2100 Copenhagen, DenmarkDepartment of Neurosurgery, Copenhagen University Hospital, Rigshospitalet, Inge Lehmanns Vej 6, 2100 Copenhagen, DenmarkSection of Biostatistics, Department of Public Health, University of Copenhagen, Øster Farimagsgade 5, 1014 Copenhagen, DenmarkPathology Department, Copenhagen University Hospital, Rigshospitalet, Inge Lehmanns Vej 7, 2100 Copenhagen, DenmarkPathology Department, Copenhagen University Hospital, Rigshospitalet, Inge Lehmanns Vej 7, 2100 Copenhagen, DenmarkDepartment of Neurosurgery, Copenhagen University Hospital, Rigshospitalet, Inge Lehmanns Vej 6, 2100 Copenhagen, DenmarkMeningiomas with inherently high mitotic indices and poor prognosis, such as WHO grade III meningiomas, have not been investigated separately to establish interchangeability between conventional mitotic index counted on H&E stained slides (MI) and mitotic index counted on phosphohistone-H3 stained slides (PHH3 MI). This study investigates the agreement of MI and PHH3 MI and to analyze the association of progression-free survival (PFS) and MI, PHH3 MI, and the proliferative index (PI, Ki-67) in WHO grade III meningioma. Tumor specimens from 24 consecutive patients were analyzed for expression of Ki-67, PHH3 MI, and MI. Quantification was performed independently by two observers who made replicate counts in hot spots and overall tumor staining. Repeatability in replicate counts from MI and PHH3 MI was low in both observers. Consequently, we could not report the agreement. MI, PHH3 MI and hot spot counts of Ki-67 were associated with PFS (MI hot spot HR = 1.61, 95% CI 1.12–2.31, <i>p</i> = 0.010; PHH3 MI hot spot HR = 1.59, 95% CI 1.15–2.21, <i>p</i> = 0.006; Ki-67 hot spot HR = 1.06, 95% CI 1.02–1.11. <i>p</i> = 0.004). We found markedly low repeatability of manually counted MI and PHH3 MI in WHO grade III meningioma, and we could not conclude that the two methods agreed. Subsequently, quantification with better repeatability should be sought. All three biomarkers were associated with PFS.https://www.mdpi.com/2072-6694/12/11/3351malignant meningiomamitotic indiceshot spotsKi-67agreement |
| spellingShingle | Andrea Daniela Maier Christian Beltoft Brøchner Jiri Bartek Jr. Frank Eriksson Heidi Ugleholdt Helle Broholm Tiit Mathiesen Mitotic and Proliferative Indices in WHO Grade III Meningioma Cancers malignant meningioma mitotic indices hot spots Ki-67 agreement |
| title | Mitotic and Proliferative Indices in WHO Grade III Meningioma |
| title_full | Mitotic and Proliferative Indices in WHO Grade III Meningioma |
| title_fullStr | Mitotic and Proliferative Indices in WHO Grade III Meningioma |
| title_full_unstemmed | Mitotic and Proliferative Indices in WHO Grade III Meningioma |
| title_short | Mitotic and Proliferative Indices in WHO Grade III Meningioma |
| title_sort | mitotic and proliferative indices in who grade iii meningioma |
| topic | malignant meningioma mitotic indices hot spots Ki-67 agreement |
| url | https://www.mdpi.com/2072-6694/12/11/3351 |
| work_keys_str_mv | AT andreadanielamaier mitoticandproliferativeindicesinwhogradeiiimeningioma AT christianbeltoftbrøchner mitoticandproliferativeindicesinwhogradeiiimeningioma AT jiribartekjr mitoticandproliferativeindicesinwhogradeiiimeningioma AT frankeriksson mitoticandproliferativeindicesinwhogradeiiimeningioma AT heidiugleholdt mitoticandproliferativeindicesinwhogradeiiimeningioma AT hellebroholm mitoticandproliferativeindicesinwhogradeiiimeningioma AT tiitmathiesen mitoticandproliferativeindicesinwhogradeiiimeningioma |