Anti-inflammatory effect of ozonated krill (Euphausia superba) oil in lipopolysaccharide-stimulated RAW 264.7 macrophages
Abstract Background Inflammation has been known to associate with many human diseases. The objective of this study was to evaluate an anti-inflammatory effect of ozonated krill (Euphausia superba) oil, which was prepared by the treatment of krill oil using ozone gas. The anti-inflammatory activity w...
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| Format: | Article |
| Language: | English |
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The Korean Society of Fisheries and Aquatic Science
2018-06-01
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| Series: | Fisheries and Aquatic Sciences |
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| Online Access: | http://link.springer.com/article/10.1186/s41240-018-0092-1 |
| _version_ | 1818434953854058496 |
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| author | Hong-Deok Kim Soo-Bin Lee Seok-Chun Ko Won-Kyo Jung Young-Mog Kim Seon-Bong Kim |
| author_facet | Hong-Deok Kim Soo-Bin Lee Seok-Chun Ko Won-Kyo Jung Young-Mog Kim Seon-Bong Kim |
| author_sort | Hong-Deok Kim |
| collection | DOAJ |
| description | Abstract Background Inflammation has been known to associate with many human diseases. The objective of this study was to evaluate an anti-inflammatory effect of ozonated krill (Euphausia superba) oil, which was prepared by the treatment of krill oil using ozone gas. The anti-inflammatory activity was evaluated in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. Results Ozonated krill oil significantly inhibited nitric oxide (NO) production and suppressed the mRNA and protein expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in LPS-stimulated RAW 264.7 macrophages. Ozonated krill oil also reduced the mRNA expression of inflammatory cytokines such as interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)-α in LPS-stimulated RAW 264.7 macrophages. To elucidate the mechanism underlying the anti-inflammatory activity of ozonated krill oil, we evaluated the effects of ozonated krill oil on the activation of mitogen-activated protein kinases (MAPKs) pathway. Ozonated krill oil suppressed the LPS-stimulated phosphorylation of p38 MAPK and c-Jun N-terminal kinases (JNK). Conclusion This study revealed that the ozonated krill oil exhibited an anti-inflammatory effect in LPS-stimulated RAW 264.7 macrophages. To the best of our knowledge, this is the first report that ozonated krill oil suppressed pro-inflammatory mediator and cytokine expression in LPS-stimulated RAW 264.7 macrophages by inhibiting the phosphorylation of p38 MAPK and JNK. |
| first_indexed | 2024-12-14T16:45:11Z |
| format | Article |
| id | doaj.art-c72a441225dd42f8a67a4ee0d8f3e93e |
| institution | Directory Open Access Journal |
| issn | 2234-1757 |
| language | English |
| last_indexed | 2024-12-14T16:45:11Z |
| publishDate | 2018-06-01 |
| publisher | The Korean Society of Fisheries and Aquatic Science |
| record_format | Article |
| series | Fisheries and Aquatic Sciences |
| spelling | doaj.art-c72a441225dd42f8a67a4ee0d8f3e93e2022-12-21T22:54:13ZengThe Korean Society of Fisheries and Aquatic ScienceFisheries and Aquatic Sciences2234-17572018-06-012111910.1186/s41240-018-0092-1Anti-inflammatory effect of ozonated krill (Euphausia superba) oil in lipopolysaccharide-stimulated RAW 264.7 macrophagesHong-Deok Kim0Soo-Bin Lee1Seok-Chun Ko2Won-Kyo Jung3Young-Mog Kim4Seon-Bong Kim5Department of Food Science and Technology, Pukyong National UniversityDepartment of Food Science and Technology, Pukyong National UniversityMarine-Intergrated Bionics Research Center, Pukyong National UniversityMarine-Intergrated Bionics Research Center, Pukyong National UniversityDepartment of Food Science and Technology, Pukyong National UniversityDepartment of Food Science and Technology, Pukyong National UniversityAbstract Background Inflammation has been known to associate with many human diseases. The objective of this study was to evaluate an anti-inflammatory effect of ozonated krill (Euphausia superba) oil, which was prepared by the treatment of krill oil using ozone gas. The anti-inflammatory activity was evaluated in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. Results Ozonated krill oil significantly inhibited nitric oxide (NO) production and suppressed the mRNA and protein expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in LPS-stimulated RAW 264.7 macrophages. Ozonated krill oil also reduced the mRNA expression of inflammatory cytokines such as interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)-α in LPS-stimulated RAW 264.7 macrophages. To elucidate the mechanism underlying the anti-inflammatory activity of ozonated krill oil, we evaluated the effects of ozonated krill oil on the activation of mitogen-activated protein kinases (MAPKs) pathway. Ozonated krill oil suppressed the LPS-stimulated phosphorylation of p38 MAPK and c-Jun N-terminal kinases (JNK). Conclusion This study revealed that the ozonated krill oil exhibited an anti-inflammatory effect in LPS-stimulated RAW 264.7 macrophages. To the best of our knowledge, this is the first report that ozonated krill oil suppressed pro-inflammatory mediator and cytokine expression in LPS-stimulated RAW 264.7 macrophages by inhibiting the phosphorylation of p38 MAPK and JNK.http://link.springer.com/article/10.1186/s41240-018-0092-1Ozonated krill oilAnti-inflammatoryRAW 264.7 macrophagesMAPKs |
| spellingShingle | Hong-Deok Kim Soo-Bin Lee Seok-Chun Ko Won-Kyo Jung Young-Mog Kim Seon-Bong Kim Anti-inflammatory effect of ozonated krill (Euphausia superba) oil in lipopolysaccharide-stimulated RAW 264.7 macrophages Fisheries and Aquatic Sciences Ozonated krill oil Anti-inflammatory RAW 264.7 macrophages MAPKs |
| title | Anti-inflammatory effect of ozonated krill (Euphausia superba) oil in lipopolysaccharide-stimulated RAW 264.7 macrophages |
| title_full | Anti-inflammatory effect of ozonated krill (Euphausia superba) oil in lipopolysaccharide-stimulated RAW 264.7 macrophages |
| title_fullStr | Anti-inflammatory effect of ozonated krill (Euphausia superba) oil in lipopolysaccharide-stimulated RAW 264.7 macrophages |
| title_full_unstemmed | Anti-inflammatory effect of ozonated krill (Euphausia superba) oil in lipopolysaccharide-stimulated RAW 264.7 macrophages |
| title_short | Anti-inflammatory effect of ozonated krill (Euphausia superba) oil in lipopolysaccharide-stimulated RAW 264.7 macrophages |
| title_sort | anti inflammatory effect of ozonated krill euphausia superba oil in lipopolysaccharide stimulated raw 264 7 macrophages |
| topic | Ozonated krill oil Anti-inflammatory RAW 264.7 macrophages MAPKs |
| url | http://link.springer.com/article/10.1186/s41240-018-0092-1 |
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