Curcumin Quantum Dots Mediated Degradation of Bacterial Biofilms
Bacterial biofilm has been reported to be associated with more than 80% of bacterial infections. Curcumin, a hydrophobic polyphenol compound, has anti-quorum sensing activity apart from having antimicrobial action. However, its use is limited by its poor aqueous solubility and rapid degradation. In...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2017-08-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fmicb.2017.01517/full |
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| author | Ashish K. Singh Ashish K. Singh Pradyot Prakash Ranjana Singh Nabarun Nandy Zeba Firdaus Monika Bansal Ranjan K. Singh Anchal Srivastava Anchal Srivastava Jagat K. Roy Brahmeshwar Mishra Rakesh K. Singh |
| author_facet | Ashish K. Singh Ashish K. Singh Pradyot Prakash Ranjana Singh Nabarun Nandy Zeba Firdaus Monika Bansal Ranjan K. Singh Anchal Srivastava Anchal Srivastava Jagat K. Roy Brahmeshwar Mishra Rakesh K. Singh |
| author_sort | Ashish K. Singh |
| collection | DOAJ |
| description | Bacterial biofilm has been reported to be associated with more than 80% of bacterial infections. Curcumin, a hydrophobic polyphenol compound, has anti-quorum sensing activity apart from having antimicrobial action. However, its use is limited by its poor aqueous solubility and rapid degradation. In this study, we attempted to prepare quantum dots of the drug curcumin in order to achieve enhanced solubility and stability and investigated for its antimicrobial and antibiofilm activity. We utilized a newer two-step bottom up wet milling approach to prepare Curcumin Quantum Dots (CurQDs) using acetone as a primary solvent. Minimum inhibitory concentration against select Gram-positive and Gram-negative bacteria was performed. The antibiofilm assay was performed at first using 96-well tissue culture plate and subsequently validated by Confocal Laser Scanning Microscopy. Further, biofilm matrix protein was isolated using formaldehyde sludge and TCA/Acetone precipitation method. Protein extracted was incubated with varying concentration of CurQDs for 4 h and was subjected to SDS–PAGE. Molecular docking study was performed to observe interaction between curcumin and phenol soluble modulins as well as curli proteins. The biophysical evidences obtained from TEM, SEM, UV-VIS, fluorescence, Raman spectroscopy, and zeta potential analysis confirmed the formation of curcumin quantum dots with increased stability and solubility. The MICs of curcumin quantum dots, as observed against both select gram positive and negative bacterial isolates, was observed to be significantly lower than native curcumin particles. On TCP assay, Curcumin observed to be having antibiofilm as well as biofilm degrading activity. Results of SDS–PAGE and molecular docking have shown interaction between biofilm matrix proteins and curcumin. The results indicate that aqueous solubility and stability of Curcumin can be achieved by preparing its quantum dots. The study also demonstrates that by sizing down the particle size has not only enhanced its antimicrobial properties but it has also shown its antibiofilm activities. Further, study is needed to elucidate the exact nature of interaction between curcumin and biofilm matrix proteins. |
| first_indexed | 2024-04-13T11:30:06Z |
| format | Article |
| id | doaj.art-c72d18d5993a40808d0606f58a66153b |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-13T11:30:06Z |
| publishDate | 2017-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-c72d18d5993a40808d0606f58a66153b2022-12-22T02:48:36ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2017-08-01810.3389/fmicb.2017.01517280452Curcumin Quantum Dots Mediated Degradation of Bacterial BiofilmsAshish K. Singh0Ashish K. Singh1Pradyot Prakash2Ranjana Singh3Nabarun Nandy4Zeba Firdaus5Monika Bansal6Ranjan K. Singh7Anchal Srivastava8Anchal Srivastava9Jagat K. Roy10Brahmeshwar Mishra11Rakesh K. Singh12Bacterial Biofilm and Drug Resistance Research Group, Department of Microbiology, Institute of Medical Sciences, Banaras Hindu UniversityVaranasi, IndiaMolecular Immunology Laboratory, Department of Biochemistry, Institute of Science, Banaras Hindu UniversityVaranasi, IndiaBacterial Biofilm and Drug Resistance Research Group, Department of Microbiology, Institute of Medical Sciences, Banaras Hindu UniversityVaranasi, IndiaBiophysics Laboratory, Department of Physics, Institute of Science, Banaras Hindu UniversityVaranasi, IndiaCytogenetics Laboratory, Department of Zoology, Institute of Science, Banaras Hindu UniversityVaranasi, IndiaDepartment of Medicinal Chemistry, Institute of Medical Sciences, Banaras Hindu UniversityVaranasi, IndiaFaculty of Dental Sciences, Institute of Medical Sciences, Banaras Hindu UniversityVaranasi, IndiaBiophysics Laboratory, Department of Physics, Institute of Science, Banaras Hindu UniversityVaranasi, IndiaBiophysics Laboratory, Department of Physics, Institute of Science, Banaras Hindu UniversityVaranasi, IndiaNano Research Laboratory, Department of Physics, Banaras Hindu UniversityVaranasi, IndiaCytogenetics Laboratory, Department of Zoology, Institute of Science, Banaras Hindu UniversityVaranasi, IndiaDepartment of Pharmaceutics, Indian Institute of Technology, Banaras Hindu UniversityVaranasi, IndiaMolecular Immunology Laboratory, Department of Biochemistry, Institute of Science, Banaras Hindu UniversityVaranasi, IndiaBacterial biofilm has been reported to be associated with more than 80% of bacterial infections. Curcumin, a hydrophobic polyphenol compound, has anti-quorum sensing activity apart from having antimicrobial action. However, its use is limited by its poor aqueous solubility and rapid degradation. In this study, we attempted to prepare quantum dots of the drug curcumin in order to achieve enhanced solubility and stability and investigated for its antimicrobial and antibiofilm activity. We utilized a newer two-step bottom up wet milling approach to prepare Curcumin Quantum Dots (CurQDs) using acetone as a primary solvent. Minimum inhibitory concentration against select Gram-positive and Gram-negative bacteria was performed. The antibiofilm assay was performed at first using 96-well tissue culture plate and subsequently validated by Confocal Laser Scanning Microscopy. Further, biofilm matrix protein was isolated using formaldehyde sludge and TCA/Acetone precipitation method. Protein extracted was incubated with varying concentration of CurQDs for 4 h and was subjected to SDS–PAGE. Molecular docking study was performed to observe interaction between curcumin and phenol soluble modulins as well as curli proteins. The biophysical evidences obtained from TEM, SEM, UV-VIS, fluorescence, Raman spectroscopy, and zeta potential analysis confirmed the formation of curcumin quantum dots with increased stability and solubility. The MICs of curcumin quantum dots, as observed against both select gram positive and negative bacterial isolates, was observed to be significantly lower than native curcumin particles. On TCP assay, Curcumin observed to be having antibiofilm as well as biofilm degrading activity. Results of SDS–PAGE and molecular docking have shown interaction between biofilm matrix proteins and curcumin. The results indicate that aqueous solubility and stability of Curcumin can be achieved by preparing its quantum dots. The study also demonstrates that by sizing down the particle size has not only enhanced its antimicrobial properties but it has also shown its antibiofilm activities. Further, study is needed to elucidate the exact nature of interaction between curcumin and biofilm matrix proteins.http://journal.frontiersin.org/article/10.3389/fmicb.2017.01517/fulladhesionbacterial biofilmcurcuminantimicrobial agentsnano-curcuminquantum dots |
| spellingShingle | Ashish K. Singh Ashish K. Singh Pradyot Prakash Ranjana Singh Nabarun Nandy Zeba Firdaus Monika Bansal Ranjan K. Singh Anchal Srivastava Anchal Srivastava Jagat K. Roy Brahmeshwar Mishra Rakesh K. Singh Curcumin Quantum Dots Mediated Degradation of Bacterial Biofilms Frontiers in Microbiology adhesion bacterial biofilm curcumin antimicrobial agents nano-curcumin quantum dots |
| title | Curcumin Quantum Dots Mediated Degradation of Bacterial Biofilms |
| title_full | Curcumin Quantum Dots Mediated Degradation of Bacterial Biofilms |
| title_fullStr | Curcumin Quantum Dots Mediated Degradation of Bacterial Biofilms |
| title_full_unstemmed | Curcumin Quantum Dots Mediated Degradation of Bacterial Biofilms |
| title_short | Curcumin Quantum Dots Mediated Degradation of Bacterial Biofilms |
| title_sort | curcumin quantum dots mediated degradation of bacterial biofilms |
| topic | adhesion bacterial biofilm curcumin antimicrobial agents nano-curcumin quantum dots |
| url | http://journal.frontiersin.org/article/10.3389/fmicb.2017.01517/full |
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