Phytochemical and Antiplasmodial Evaluation of Five Colombian Plants with Ethnopharmacological Background of Antimalarial Use
Background: The study of medicinal plants has made it possible to develop products and drugs for the treatment of different diseases. Several plants in Colombia have a history of popular use for the treatment of malaria. The objective of this work was to provide information on the antiplasmodic and...
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| Format: | Article |
| Language: | English |
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Tabriz University of Medical Sciences
2023-01-01
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| Series: | Pharmaceutical Sciences |
| Subjects: | |
| Online Access: | https://ps.tbzmed.ac.ir/PDF/ps-29-123.pdf |
| _version_ | 1797846182221840384 |
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| author | Jorge Enrique Hernández-Carvajal María Helena Arias-Marciales José Octavio García Yoshie Adriana Hata-Uribe Giovanny Garavito-Cárdenas Pilar Ester Luengas Caicedo |
| author_facet | Jorge Enrique Hernández-Carvajal María Helena Arias-Marciales José Octavio García Yoshie Adriana Hata-Uribe Giovanny Garavito-Cárdenas Pilar Ester Luengas Caicedo |
| author_sort | Jorge Enrique Hernández-Carvajal |
| collection | DOAJ |
| description | Background: The study of medicinal plants has made it possible to develop products and drugs for the treatment of different diseases. Several plants in Colombia have a history of popular use for the treatment of malaria. The objective of this work was to provide information on the antiplasmodic and phytochemical activity of five neotropical native plants with a folk use for the treatment of malaria. Methods: The ethanolic extract of each species was obtained by percolation method and characterized by thin-layer chromatography (TLC), high-performance liquid chromatography (HPLC), and hydrogen nuclear magnetic resonance (1H-NMR). The in vitro antiplasmodial activity was evaluated against Plasmodium falciparum (strain FCR-3, chloroquine-resistant). Results: Ethanolic extracts of Ambelania duckey, Cecropia metensis, Cecropia membranacea, and Verbena littoralis showed no activity. However, Curarea toxicofera extract exhibited an IC50 of 7.6 ± 3.9 μg/mL and was classified as moderately active. Most extracts show hemolytic concentration (CH50) > 1000 μg/ mL). A preliminary phytochemical study was carried out using tube analysis, TLC, HPLC, and 1H-NMR. Steroids or triterpenes, and phenolic compounds were detected by TLC in all extracts. These findings were confirmed by characteristic aliphatic and aromatic signals in 1H-NMR spectra, corresponding to triterpenes and phenolics, respectively. Additionally, alkaloids were extracted from C. toxicofera and detected by aromatic signals in 1H-NMR spectra. Conclusion: Ethanolic extract of C. toxicofera showed antiplasmodial activity (IC50 of 7.6 ± 3.9 μg/mL); this activity may be due to the bisbenzylisoquinoline alkaloids. The ethanol extracts of A. duckey, C. membranacea, C. metensis, and V. littoralis did not present antiplasmodial activity. |
| first_indexed | 2024-04-09T17:50:46Z |
| format | Article |
| id | doaj.art-c72e065c302c4640a6d0ea24898e2e85 |
| institution | Directory Open Access Journal |
| issn | 2383-2886 |
| language | English |
| last_indexed | 2024-04-09T17:50:46Z |
| publishDate | 2023-01-01 |
| publisher | Tabriz University of Medical Sciences |
| record_format | Article |
| series | Pharmaceutical Sciences |
| spelling | doaj.art-c72e065c302c4640a6d0ea24898e2e852023-04-16T08:18:29ZengTabriz University of Medical SciencesPharmaceutical Sciences2383-28862023-01-0129112313210.34172/PS.2022.16ps-34866Phytochemical and Antiplasmodial Evaluation of Five Colombian Plants with Ethnopharmacological Background of Antimalarial UseJorge Enrique Hernández-Carvajal0María Helena Arias-Marciales1José Octavio García2Yoshie Adriana Hata-Uribe3Giovanny Garavito-Cárdenas4Pilar Ester Luengas Caicedo5Universidad Nacional de Colombia – Sede Bogotá, Facultad de Ciencias, Departamento de Farmacia, Bogotá D.C., Colombia.Universidad Nacional de Colombia – Sede Bogotá, Facultad de Ciencias, Departamento de Farmacia, Bogotá D.C., Colombia.Uitoto representative, Comunidad Ciudad Hitoma, Km 7 Via Leticia - Tarapaca, Leticia, Colombia (RIP).Universidad Nacional de Colombia – Sede Bogotá, Facultad de Ciencias, Departamento de Farmacia, Bogotá D.C., Colombia.Universidad Nacional de Colombia – Sede Bogotá, Facultad de Ciencias, Departamento de Farmacia, Bogotá D.C., Colombia.Universidad Nacional de Colombia – Sede Bogotá, Facultad de Ciencias, Departamento de Farmacia, Bogotá D.C., Colombia.Background: The study of medicinal plants has made it possible to develop products and drugs for the treatment of different diseases. Several plants in Colombia have a history of popular use for the treatment of malaria. The objective of this work was to provide information on the antiplasmodic and phytochemical activity of five neotropical native plants with a folk use for the treatment of malaria. Methods: The ethanolic extract of each species was obtained by percolation method and characterized by thin-layer chromatography (TLC), high-performance liquid chromatography (HPLC), and hydrogen nuclear magnetic resonance (1H-NMR). The in vitro antiplasmodial activity was evaluated against Plasmodium falciparum (strain FCR-3, chloroquine-resistant). Results: Ethanolic extracts of Ambelania duckey, Cecropia metensis, Cecropia membranacea, and Verbena littoralis showed no activity. However, Curarea toxicofera extract exhibited an IC50 of 7.6 ± 3.9 μg/mL and was classified as moderately active. Most extracts show hemolytic concentration (CH50) > 1000 μg/ mL). A preliminary phytochemical study was carried out using tube analysis, TLC, HPLC, and 1H-NMR. Steroids or triterpenes, and phenolic compounds were detected by TLC in all extracts. These findings were confirmed by characteristic aliphatic and aromatic signals in 1H-NMR spectra, corresponding to triterpenes and phenolics, respectively. Additionally, alkaloids were extracted from C. toxicofera and detected by aromatic signals in 1H-NMR spectra. Conclusion: Ethanolic extract of C. toxicofera showed antiplasmodial activity (IC50 of 7.6 ± 3.9 μg/mL); this activity may be due to the bisbenzylisoquinoline alkaloids. The ethanol extracts of A. duckey, C. membranacea, C. metensis, and V. littoralis did not present antiplasmodial activity.https://ps.tbzmed.ac.ir/PDF/ps-29-123.pdfcurarea toxicoferaantiplasmodialphytochemicalsplasmodium falciparumcolombian plantsethnopharmacological |
| spellingShingle | Jorge Enrique Hernández-Carvajal María Helena Arias-Marciales José Octavio García Yoshie Adriana Hata-Uribe Giovanny Garavito-Cárdenas Pilar Ester Luengas Caicedo Phytochemical and Antiplasmodial Evaluation of Five Colombian Plants with Ethnopharmacological Background of Antimalarial Use Pharmaceutical Sciences curarea toxicofera antiplasmodial phytochemicals plasmodium falciparum colombian plants ethnopharmacological |
| title | Phytochemical and Antiplasmodial Evaluation of Five Colombian Plants with Ethnopharmacological Background of Antimalarial Use |
| title_full | Phytochemical and Antiplasmodial Evaluation of Five Colombian Plants with Ethnopharmacological Background of Antimalarial Use |
| title_fullStr | Phytochemical and Antiplasmodial Evaluation of Five Colombian Plants with Ethnopharmacological Background of Antimalarial Use |
| title_full_unstemmed | Phytochemical and Antiplasmodial Evaluation of Five Colombian Plants with Ethnopharmacological Background of Antimalarial Use |
| title_short | Phytochemical and Antiplasmodial Evaluation of Five Colombian Plants with Ethnopharmacological Background of Antimalarial Use |
| title_sort | phytochemical and antiplasmodial evaluation of five colombian plants with ethnopharmacological background of antimalarial use |
| topic | curarea toxicofera antiplasmodial phytochemicals plasmodium falciparum colombian plants ethnopharmacological |
| url | https://ps.tbzmed.ac.ir/PDF/ps-29-123.pdf |
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