Genotype Change in Circulating JEV Strains in Fujian Province, China
Japanese encephalitis (JE), found in pigs, is a serious mosquito-borne zoonotic infectious disease caused by the Japanese encephalitis virus (JEV). JEV is maintained in an enzootic cycle between mosquitoes and amplifying vertebrate hosts, mainly pigs and wading birds. It is transmitted to humans thr...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-08-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/15/9/1822 |
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| author | Nihua Dong Xinya Zhang Hailong Zhang Jiayang Zheng Yafeng Qiu Zongjie Li Beibei Li Ke Liu Donghua Shao Zhiyong Ma Jianchao Wei |
| author_facet | Nihua Dong Xinya Zhang Hailong Zhang Jiayang Zheng Yafeng Qiu Zongjie Li Beibei Li Ke Liu Donghua Shao Zhiyong Ma Jianchao Wei |
| author_sort | Nihua Dong |
| collection | DOAJ |
| description | Japanese encephalitis (JE), found in pigs, is a serious mosquito-borne zoonotic infectious disease caused by the Japanese encephalitis virus (JEV). JEV is maintained in an enzootic cycle between mosquitoes and amplifying vertebrate hosts, mainly pigs and wading birds. It is transmitted to humans through the bite of an infected mosquito, allowing the pathogen to spread and cause disease epidemics. However, there is little research on JEV genotype variation in mosquitoes and pigs in Fujian province. Previous studies have shown that the main epidemic strain of JEV in Fujian Province is genotype III. In this study, a survey of mosquito species diversity in pig farms and molecular evolutionary analyses of JEV were conducted in Fujian, China, in the summer of 2019. A total of 19,177 mosquitoes were collected at four sites by UV trap. Four genera were identified, of which the <i>Culex tritaeniorhynchus</i> was the most common mosquito species, accounting for 76.4% of the total (14,651/19,177). <i>Anopheles sinensi</i> (19.25%, 3691/19,177) was the second largest species. High mosquito infection rateswere an important factor in the outbreak. The captured mosquito samples were milled and screened with JEV-specific primers. Five viruses were isolated, FJ1901, FJ1902, FJ1903, FJ1904, and FJ1905. Genetic affinity was determined by analyzing the envelope (E) gene variants. The results showed that they are JEV gene type I and most closely related to the strains SH-53 and SD0810. In this study, it was found through genetic evolution analysis that the main epidemic strain of JE in pig farms changed from gene type III to gene type I. Compared with the SH-53 and SD0810 strains, we found no change in key sites related to antigenic activity and neurovirulence of JEV in Fujian JEV and pig mosquito strains, respectively. The results of the study provide basic data for analyzing the genotypic shift of JEV in Fujian Province and support the prevention and control of JEV. |
| first_indexed | 2024-03-10T21:52:54Z |
| format | Article |
| id | doaj.art-c72e623416dd4d37b279d1c56b4e5252 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-10T21:52:54Z |
| publishDate | 2023-08-01 |
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| record_format | Article |
| series | Viruses |
| spelling | doaj.art-c72e623416dd4d37b279d1c56b4e52522023-11-19T13:22:09ZengMDPI AGViruses1999-49152023-08-01159182210.3390/v15091822Genotype Change in Circulating JEV Strains in Fujian Province, ChinaNihua Dong0Xinya Zhang1Hailong Zhang2Jiayang Zheng3Yafeng Qiu4Zongjie Li5Beibei Li6Ke Liu7Donghua Shao8Zhiyong Ma9Jianchao Wei10Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Science, Shanghai 200241, ChinaShanghai Veterinary Research Institute, Chinese Academy of Agricultural Science, Shanghai 200241, ChinaShanghai Veterinary Research Institute, Chinese Academy of Agricultural Science, Shanghai 200241, ChinaShanghai Veterinary Research Institute, Chinese Academy of Agricultural Science, Shanghai 200241, ChinaShanghai Veterinary Research Institute, Chinese Academy of Agricultural Science, Shanghai 200241, ChinaShanghai Veterinary Research Institute, Chinese Academy of Agricultural Science, Shanghai 200241, ChinaShanghai Veterinary Research Institute, Chinese Academy of Agricultural Science, Shanghai 200241, ChinaShanghai Veterinary Research Institute, Chinese Academy of Agricultural Science, Shanghai 200241, ChinaShanghai Veterinary Research Institute, Chinese Academy of Agricultural Science, Shanghai 200241, ChinaShanghai Veterinary Research Institute, Chinese Academy of Agricultural Science, Shanghai 200241, ChinaShanghai Veterinary Research Institute, Chinese Academy of Agricultural Science, Shanghai 200241, ChinaJapanese encephalitis (JE), found in pigs, is a serious mosquito-borne zoonotic infectious disease caused by the Japanese encephalitis virus (JEV). JEV is maintained in an enzootic cycle between mosquitoes and amplifying vertebrate hosts, mainly pigs and wading birds. It is transmitted to humans through the bite of an infected mosquito, allowing the pathogen to spread and cause disease epidemics. However, there is little research on JEV genotype variation in mosquitoes and pigs in Fujian province. Previous studies have shown that the main epidemic strain of JEV in Fujian Province is genotype III. In this study, a survey of mosquito species diversity in pig farms and molecular evolutionary analyses of JEV were conducted in Fujian, China, in the summer of 2019. A total of 19,177 mosquitoes were collected at four sites by UV trap. Four genera were identified, of which the <i>Culex tritaeniorhynchus</i> was the most common mosquito species, accounting for 76.4% of the total (14,651/19,177). <i>Anopheles sinensi</i> (19.25%, 3691/19,177) was the second largest species. High mosquito infection rateswere an important factor in the outbreak. The captured mosquito samples were milled and screened with JEV-specific primers. Five viruses were isolated, FJ1901, FJ1902, FJ1903, FJ1904, and FJ1905. Genetic affinity was determined by analyzing the envelope (E) gene variants. The results showed that they are JEV gene type I and most closely related to the strains SH-53 and SD0810. In this study, it was found through genetic evolution analysis that the main epidemic strain of JE in pig farms changed from gene type III to gene type I. Compared with the SH-53 and SD0810 strains, we found no change in key sites related to antigenic activity and neurovirulence of JEV in Fujian JEV and pig mosquito strains, respectively. The results of the study provide basic data for analyzing the genotypic shift of JEV in Fujian Province and support the prevention and control of JEV.https://www.mdpi.com/1999-4915/15/9/1822JEVFujianmosquito |
| spellingShingle | Nihua Dong Xinya Zhang Hailong Zhang Jiayang Zheng Yafeng Qiu Zongjie Li Beibei Li Ke Liu Donghua Shao Zhiyong Ma Jianchao Wei Genotype Change in Circulating JEV Strains in Fujian Province, China Viruses JEV Fujian mosquito |
| title | Genotype Change in Circulating JEV Strains in Fujian Province, China |
| title_full | Genotype Change in Circulating JEV Strains in Fujian Province, China |
| title_fullStr | Genotype Change in Circulating JEV Strains in Fujian Province, China |
| title_full_unstemmed | Genotype Change in Circulating JEV Strains in Fujian Province, China |
| title_short | Genotype Change in Circulating JEV Strains in Fujian Province, China |
| title_sort | genotype change in circulating jev strains in fujian province china |
| topic | JEV Fujian mosquito |
| url | https://www.mdpi.com/1999-4915/15/9/1822 |
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