Excitable Adult-Generated GABAergic Neurons Acquire Functional Innervation in the Cortex after Stroke
Summary: Ischemic stroke enhances the proliferation of adult-generated precursor cells that ectopically migrate toward the infarct. Studies have correlated precursor cell proliferation and subsequent adult neurogenesis with enhanced stroke recovery, yet it remains unclear whether stroke can generate...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2018-12-01
|
| Series: | Stem Cell Reports |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S221367111830434X |
| _version_ | 1818060260026351616 |
|---|---|
| author | Timal S. Kannangara Anthony Carter Yingben Xue Jagroop S. Dhaliwal Jean-Claude Béïque Diane C. Lagace |
| author_facet | Timal S. Kannangara Anthony Carter Yingben Xue Jagroop S. Dhaliwal Jean-Claude Béïque Diane C. Lagace |
| author_sort | Timal S. Kannangara |
| collection | DOAJ |
| description | Summary: Ischemic stroke enhances the proliferation of adult-generated precursor cells that ectopically migrate toward the infarct. Studies have correlated precursor cell proliferation and subsequent adult neurogenesis with enhanced stroke recovery, yet it remains unclear whether stroke can generate new neurons capable of functional integration into the injured cortex. Here, using single and bitransgenic reporter mice, we identify spatial and temporal features of a multilineage cellular response to focal ischemia. We reveal that a small population of stroke-induced immature neurons accumulate within the peri-infarct region of the adult sensorimotor cortex, exhibit voltage-dependent conductances, fire action potentials, express GABAergic markers, and receive sparse GABAergic synaptic inputs. Collectively, these findings reveal that GABAergic neurons arising from the lateral ventricle have the capacity to integrate into the stroke-injured cortex, although their limited number and exiguous synaptic integration may limit their ability to participate in stroke recovery. : In this article, Kannangara and colleagues histologically and electrophysiologically characterized the precursor cell response evoked in the adult cortex after focal ischemia. The authors identify a population of doublecortin-expressing GABAergic immature neurons localized to the injured cortical regions with the capacity to fire action potentials and receive GABAergic input, indicative of functionally integration into the cortical network. Keywords: stroke, neurogenesis, GABA, cortex, ischemia, injury, photothrombosis, stem cells, brain repair, subventricular zone |
| first_indexed | 2024-12-10T13:29:35Z |
| format | Article |
| id | doaj.art-c73212abef8c4d01b37b01d54a696e56 |
| institution | Directory Open Access Journal |
| issn | 2213-6711 |
| language | English |
| last_indexed | 2024-12-10T13:29:35Z |
| publishDate | 2018-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Reports |
| spelling | doaj.art-c73212abef8c4d01b37b01d54a696e562022-12-22T01:47:03ZengElsevierStem Cell Reports2213-67112018-12-0111613271336Excitable Adult-Generated GABAergic Neurons Acquire Functional Innervation in the Cortex after StrokeTimal S. Kannangara0Anthony Carter1Yingben Xue2Jagroop S. Dhaliwal3Jean-Claude Béïque4Diane C. Lagace5Department of Cellular and Molecular Medicine, Faculty of Medicine, University of Ottawa, Ottawa, ON K1H 8M5, Canada; University of Ottawa Brain and Mind Research Institute, Ottawa, ON K1H 8M5, Canada; Neuroscience Program, University of Ottawa, Ottawa, ON K1H 8M5, Canada; Canadian Partnership for Stroke Recovery, Ottawa, ON K1G 5Z3, CanadaDepartment of Cellular and Molecular Medicine, Faculty of Medicine, University of Ottawa, Ottawa, ON K1H 8M5, Canada; Canadian Partnership for Stroke Recovery, Ottawa, ON K1G 5Z3, CanadaDepartment of Cellular and Molecular Medicine, Faculty of Medicine, University of Ottawa, Ottawa, ON K1H 8M5, CanadaDepartment of Cellular and Molecular Medicine, Faculty of Medicine, University of Ottawa, Ottawa, ON K1H 8M5, Canada; University of Ottawa Brain and Mind Research Institute, Ottawa, ON K1H 8M5, Canada; Neuroscience Program, University of Ottawa, Ottawa, ON K1H 8M5, CanadaDepartment of Cellular and Molecular Medicine, Faculty of Medicine, University of Ottawa, Ottawa, ON K1H 8M5, Canada; University of Ottawa Brain and Mind Research Institute, Ottawa, ON K1H 8M5, Canada; Neuroscience Program, University of Ottawa, Ottawa, ON K1H 8M5, Canada; Canadian Partnership for Stroke Recovery, Ottawa, ON K1G 5Z3, Canada; Corresponding authorDepartment of Cellular and Molecular Medicine, Faculty of Medicine, University of Ottawa, Ottawa, ON K1H 8M5, Canada; University of Ottawa Brain and Mind Research Institute, Ottawa, ON K1H 8M5, Canada; Neuroscience Program, University of Ottawa, Ottawa, ON K1H 8M5, Canada; Canadian Partnership for Stroke Recovery, Ottawa, ON K1G 5Z3, Canada; Corresponding authorSummary: Ischemic stroke enhances the proliferation of adult-generated precursor cells that ectopically migrate toward the infarct. Studies have correlated precursor cell proliferation and subsequent adult neurogenesis with enhanced stroke recovery, yet it remains unclear whether stroke can generate new neurons capable of functional integration into the injured cortex. Here, using single and bitransgenic reporter mice, we identify spatial and temporal features of a multilineage cellular response to focal ischemia. We reveal that a small population of stroke-induced immature neurons accumulate within the peri-infarct region of the adult sensorimotor cortex, exhibit voltage-dependent conductances, fire action potentials, express GABAergic markers, and receive sparse GABAergic synaptic inputs. Collectively, these findings reveal that GABAergic neurons arising from the lateral ventricle have the capacity to integrate into the stroke-injured cortex, although their limited number and exiguous synaptic integration may limit their ability to participate in stroke recovery. : In this article, Kannangara and colleagues histologically and electrophysiologically characterized the precursor cell response evoked in the adult cortex after focal ischemia. The authors identify a population of doublecortin-expressing GABAergic immature neurons localized to the injured cortical regions with the capacity to fire action potentials and receive GABAergic input, indicative of functionally integration into the cortical network. Keywords: stroke, neurogenesis, GABA, cortex, ischemia, injury, photothrombosis, stem cells, brain repair, subventricular zonehttp://www.sciencedirect.com/science/article/pii/S221367111830434X |
| spellingShingle | Timal S. Kannangara Anthony Carter Yingben Xue Jagroop S. Dhaliwal Jean-Claude Béïque Diane C. Lagace Excitable Adult-Generated GABAergic Neurons Acquire Functional Innervation in the Cortex after Stroke Stem Cell Reports |
| title | Excitable Adult-Generated GABAergic Neurons Acquire Functional Innervation in the Cortex after Stroke |
| title_full | Excitable Adult-Generated GABAergic Neurons Acquire Functional Innervation in the Cortex after Stroke |
| title_fullStr | Excitable Adult-Generated GABAergic Neurons Acquire Functional Innervation in the Cortex after Stroke |
| title_full_unstemmed | Excitable Adult-Generated GABAergic Neurons Acquire Functional Innervation in the Cortex after Stroke |
| title_short | Excitable Adult-Generated GABAergic Neurons Acquire Functional Innervation in the Cortex after Stroke |
| title_sort | excitable adult generated gabaergic neurons acquire functional innervation in the cortex after stroke |
| url | http://www.sciencedirect.com/science/article/pii/S221367111830434X |
| work_keys_str_mv | AT timalskannangara excitableadultgeneratedgabaergicneuronsacquirefunctionalinnervationinthecortexafterstroke AT anthonycarter excitableadultgeneratedgabaergicneuronsacquirefunctionalinnervationinthecortexafterstroke AT yingbenxue excitableadultgeneratedgabaergicneuronsacquirefunctionalinnervationinthecortexafterstroke AT jagroopsdhaliwal excitableadultgeneratedgabaergicneuronsacquirefunctionalinnervationinthecortexafterstroke AT jeanclaudebeique excitableadultgeneratedgabaergicneuronsacquirefunctionalinnervationinthecortexafterstroke AT dianeclagace excitableadultgeneratedgabaergicneuronsacquirefunctionalinnervationinthecortexafterstroke |